CAJ | 학술논문

病原菌的相互作用组学研究是揭示潜在信号转导通路和新型药物靶标的有力工具。通过同源蛋白映射的方法构建了牛支原体的蛋白-蛋白相互作用网络，所构建的互作网络包含138个蛋白和693个相互作用关系。通过蛋白的互作频率分析，确定了在牛支原体中互作频率最高的20个蛋白，这些蛋白主要与转录、翻译、分子伴侣等功能相关。进一步分析发现，伴侣蛋白 DnaK 和 ClpB 发生互作的频率较高，能与核糖体蛋白、代谢相关蛋白等多种功能蛋白发生相互作用。结果提示，若 DnaK 和 ClpB 的功能受到抑制，将会对牛支原体的正常生命活动造成较大的影响。
병원균적상호작용조학연구시게시잠재신호전도통로화신형약물파표적유력공구。통과동원단백영사적방법구건료우지원체적단백-단백상호작용망락，소구건적호작망락포함138개단백화693개상호작용관계。통과단백적호작빈솔분석，학정료재우지원체중호작빈솔최고적20개단백，저사단백주요여전록、번역、분자반려등공능상관。진일보분석발현，반려단백 DnaK 화 ClpB 발생호작적빈솔교고，능여핵당체단백、대사상관단백등다충공능단백발생상호작용。결과제시，약 DnaK 화 ClpB 적공능수도억제，장회대우지원체적정상생명활동조성교대적영향。
Analysis of the pathogen interactome is a powerfull approach for dissecting potential signal tras-ducion and new drug targets.We constructed a comprehensive protein-protein interaction network for My-coplasma bovis consisting of 138 proteins and 693 interaction pairs.Our analysis unraveled 20 proteins with highest interactions in the network,most of them are related to the function of transcription,translation and molecular chaperone.The MMB0149/MMB0664 gene and its gene product DnaK/ClpB were analyzed in detail because they showed high interactions with ribosomal and metabolism related proteins.Our analy-ses indicated that the chaperone DnaK and ClpB may be critical for the survival of Mycoplasma bovis .Col-lectively,this study therefore provides valuable clues in exploring new signaling protein and new drug tar-gets.