CAJ | 학술논문

为研究番鸭细小病毒基因遗传变异特征，通过 PCR技术获得了番鸭细小病毒结构蛋白基因VP3序列 AH-MDPV-VP3，并将该序列与 GenBank 数据库中登录的9条番鸭、鹅和鸭细小病毒基因相应序列进行了比对分析。测序结果可见番鸭细小病毒 VP3基因全长1605 bp，编码534个氨基酸。氨基酸序列同源性和进化分析表明 AH-MDPV-VP3与国内两个番鸭细小病毒基因 KC171936和 KM093740同源性较高，分别为100％和98．3％，且三者之间亲缘关系较近，来源于共同祖先；而与鹅细小病毒，尤其与台湾分离的鸭细小病毒基因 AY382891和 AY382892同源性相对较低，分别为91．6％和90．8％，遗传距离也相对较远。同时可见番鸭、鹅和鸭细小病毒的 VP3基因各自较为保守，仅有少数氨基酸发生变异。本研究初步获得番鸭细小病毒 VP3基因分子特征，为下一步该基因在免疫诊断及免疫防治中的应用奠定了基础。
위연구번압세소병독기인유전변이특정，통과 PCR기술획득료번압세소병독결구단백기인VP3서렬 AH-MDPV-VP3，병장해서렬여 GenBank 수거고중등록적9조번압、아화압세소병독기인상응서렬진행료비대분석。측서결과가견번압세소병독 VP3기인전장1605 bp，편마534개안기산。안기산서렬동원성화진화분석표명 AH-MDPV-VP3여국내량개번압세소병독기인 KC171936화 KM093740동원성교고，분별위100％화98．3％，차삼자지간친연관계교근，래원우공동조선；이여아세소병독，우기여태만분리적압세소병독기인 AY382891화 AY382892동원성상대교저，분별위91．6％화90．8％，유전거리야상대교원。동시가견번압、아화압세소병독적 VP3기인각자교위보수，부유소수안기산발생변이。본연구초보획득번압세소병독 VP3기인분자특정，위하일보해기인재면역진단급면역방치중적응용전정료기출。
To investigate variation status of muscovy duck parvovirus gene,the VP3 gene was cloned by PCR,which we named as AH-MDPV-VP3.And we compared AH-MDPV-VP3 with the sequence of nine VP3 genes of muscovy duck,goose and duck parvovirus in the GenBank database.By alignment of the ten gene sequences,the higher homologies between AH-MDPV-VP3 and the KC171936 and KM093740 se-quences of muscovy duck parvovirus isolated in China were found,100% and 98.3% repectively,indcating a close genetic relationship with a common ancestor of evolution.And the homologies with goose parvovir-us were lower,particularly,the AY382891 and AY382892 sequences of duck parvovirus isolated in Taiwan were 91.6% and 90.8%,the genetic distances also were farther.Moreover the VP3 gene of muscovy duck,goose and duck parvovirus were relatively conservative with several amino acid mutations.The mo-lecular characteristics of muscovy duck parvovirus VP3 gene were preliminarily obtained.And this study laid the foundation of immune diagnosis and prevention and control of muscovy duck parvovirus.