CAJ | 학술논문

为了研究丙二醛( MDA )对草鱼肠道、肝胰脏抗氧化防御能力的影响,以谷胱甘肽(GSH)/谷胱甘肽转移酶(GSTs)通路为研究对象,选择初始体重(74.8±1.0) g的草鱼(Cteno-pharyngodon idelluspond) ,随机分为4组,每组设3个重复,每个重复20尾. 4组草鱼分别投喂基础饲料(对照组)以及在基础饲料中添加61 ( B1 组)、124 ( B2 组)、185 mg/kg ( B3 组) MDA的试验饲料,在池塘网箱养殖72 d后,测定肠道、肝胰脏和血清中MDA和GSH含量,采用荧光定量PCR( qRT-PCR)方法测定草鱼肠道、肝胰脏GSH/GSTs通路中谷氨酸-半胱氨酸连接酶催化亚基( GCLC)、谷胱甘肽还原酶( GSR)、pi-谷胱甘肽硫转移酶( GSTpi)、微粒体谷胱甘肽硫转移酶1( MGSt1)基因表达量. 结果显示:1)与对照组相比,除B3组肝胰脏MDA含量显著升高( P<0.05)外,其余试验组肠道、肝胰脏MDA含量均无显著变化( P>0.05);各试验组血清MDA含量均显著升高(P<0.05). 2)与对照组相比,除B1、B3组肠道GSH含量显著升高(P<0.05)外,其余试验组肠道、肝胰脏GSH含量均无显著变化( P>0.05);B1、B2组血清GSH含量显著升高( P<0.05). 3)与对照组相比, B2、B3 组肠道及 B1 组肝胰脏 GCLC 表达量显著上调( P<0.05);除B2组肠道GSR表达量显著上调( P<0.05)外,其余试验组肠道和肝胰脏GSR表达量均无显著变化( P>0.05);B2、B3组肠道及B3组肝胰脏GSTpi表达量显著上调( P<0.05);B3组肠道MGST1表达量显著上调( P<0.05);各试验组肝胰脏MGST1表达量均显著下调( P<0.05).结果表明, MDA引起草鱼肠道、肝胰脏 GSH/GSTs通路抗氧化应激反应,且肠道和肝胰脏受MDA的影响程度有一定的差异.
위료연구병이철( MDA )대초어장도、간이장항양화방어능력적영향,이곡광감태(GSH)/곡광감태전이매(GSTs)통로위연구대상,선택초시체중(74.8±1.0) g적초어(Cteno-pharyngodon idelluspond) ,수궤분위4조,매조설3개중복,매개중복20미. 4조초어분별투위기출사료(대조조)이급재기출사료중첨가61 ( B1 조)、124 ( B2 조)、185 mg/kg ( B3 조) MDA적시험사료,재지당망상양식72 d후,측정장도、간이장화혈청중MDA화GSH함량,채용형광정량PCR( qRT-PCR)방법측정초어장도、간이장GSH/GSTs통로중곡안산-반광안산련접매최화아기( GCLC)、곡광감태환원매( GSR)、pi-곡광감태류전이매( GSTpi)、미립체곡광감태류전이매1( MGSt1)기인표체량. 결과현시:1)여대조조상비,제B3조간이장MDA함량현저승고( P<0.05)외,기여시험조장도、간이장MDA함량균무현저변화( P>0.05);각시험조혈청MDA함량균현저승고(P<0.05). 2)여대조조상비,제B1、B3조장도GSH함량현저승고(P<0.05)외,기여시험조장도、간이장GSH함량균무현저변화( P>0.05);B1、B2조혈청GSH함량현저승고( P<0.05). 3)여대조조상비, B2、B3 조장도급 B1 조간이장 GCLC 표체량현저상조( P<0.05);제B2조장도GSR표체량현저상조( P<0.05)외,기여시험조장도화간이장GSR표체량균무현저변화( P>0.05);B2、B3조장도급B3조간이장GSTpi표체량현저상조( P<0.05);B3조장도MGST1표체량현저상조( P<0.05);각시험조간이장MGST1표체량균현저하조( P<0.05).결과표명, MDA인기초어장도、간이장 GSH/GSTs통로항양화응격반응,차장도화간이장수MDA적영향정도유일정적차이.
In order to study the effects of malondialdehyde ( MDA) on anti-oxidation capability in intestine and hepatopancreas of grass carp ( Ctenopharyngodon idellus ) , the glutathione ( GSH )/glutathione transferase (GSTs) pathway was studied. The grass carp, with an initial body weight of (74.8±1.0) g, were randomly divided into 4 groups with 3 replicates in each group and each replicate contained 20 fish. Fish in those 4 groups were fed with a basal diet ( control group) and four experimeantal diets added with 61 ( B1 group) , 124 ( B2 group) and 185 mg/kg ( B3 group) MDA, respectively. After feeding 72 days, the contents of MDA and GSH in intestine, hepatopancreas and serum were measured, and the expression levels of catalytic subunit of glutamate-cysteine ligase ( GCLC) , glutathione reductase ( GSR) , glutathione-S-transferase-pi ( GSTpi) , mi-crosomal glutathione-S-transferase 1 ( MGST1 ) genes in GSH/GSTs pathway were determined by RT-qPCR method.The results showed as follows:1) in addition to MDA content in hepatopancreas of B3 group was sig-nificantly increased (P<0.05), while the other experimental groups was not significantly different compared with control group ( P>0.05); serum MDA content of all experimental groups were significantly higher than that in control group ( P<0.05) . 2) In addition to GSH content in intestine of B1 and B3 groups was signifi-cantly increased ( P<0.05) , while the other experimental groups was not significantly different compared with control group ( P>0.05);compared with control group, serum GSH content of B1 and B2 groups was signifi-cantly increased ( P<0.05) . 3) Compared with control group, the expression level of GCLC was significantly up-regulated in intestine of B2 and B3 groups and in hepatopancreas of B1 group ( P<0.05);in addition to the expression level of GSR was significantly up-regulated in intestine of B2 group ( P<0.05) , while the other ex-perimental groups was not significantly different ( P>0.05);the expression level of GSTpi was significantly up-regulated in intestine of B2 and B3 groups and in hepatopancreas of B3 group( P<0.05); the expression level of MGST1 was significantly up-regulated in intestine of B3 group ( P<0.05); the expression level of MGST1 was significantly down-regulated in hepatopancreas of all experimental groups ( P<0.05) . The results show that MDA causes GSH/GSTs pathway oxidative stress in intestine and hepatopancreas of grass carp and impacts of MDA have some differences in intestine and hepatopancreas.