CAJ | 학술논문

本试验旨在研究饲粮维生素B6 添加水平对3~5月龄生长獭兔消化代谢和抗氧化性能的影响. 选用200只3月龄獭兔,随机分成5组(每组40个重复,每个重复1只) ,分别饲喂在基础饲粮中添加0、5、10、20和40 mg/kg维生素B6 的试验饲粮. 预试期7 d,正试期54 d. 结果表明:饲粮维生素 B6 添加水平极显著影响食入氮(P=0.004 5),显著影响可消化氮(P=0.034 4)和氮表观消化率(P=0.043 6),其中10和20 mg/kg组食入氮极显著高于其他组(P<0.01) ,可消化氮和氮表观消化率均在10 mg/kg组达到最大值,但与20 mg/kg组差异不显著(P>0.05). 饲粮维生素B6 添加水平极显著影响蛋氨酸的表观消化率(P=0.006 8),显著影响半胱氨酸(P=0.011 3)、谷氨酸(P=0.022 6)和丝氨酸的表观消化率(P=0.047 8),并均在20 mg/kg组达到最大值. 饲粮维生素B6 添加水平显著影响胰腺胰蛋白酶( P=0.035 4)和糜蛋白酶活性( P=0.043 2) ,并均在20 mg/kg组达到最高值. 饲粮维生素B6 添加水平显著影响血清总蛋白(P=0.014 3)和尿素氮含量(P=0.048 2),并分别在20 mg/kg组达到最高值和最低值. 饲粮维生素B6 添加水平极显著影响血清总抗氧化能力( P=0.004 5) ,显著影响血清谷胱甘肽过氧化物酶( P=0.044 2)和总超氧化物歧化酶活性( P=0.048 1) ,并均在20 mg/kg组达到最高值,且血清丙二醛含量在20 mg/kg组达到最低值. 综合本试验测定指标,3~5月龄生长獭兔饲粮适宜的维生素B6 添加水平为10~20 mg/kg.
본시험지재연구사량유생소B6 첨가수평대3~5월령생장달토소화대사화항양화성능적영향. 선용200지3월령달토,수궤분성5조(매조40개중복,매개중복1지) ,분별사위재기출사량중첨가0、5、10、20화40 mg/kg유생소B6 적시험사량. 예시기7 d,정시기54 d. 결과표명:사량유생소 B6 첨가수평겁현저영향식입담(P=0.004 5),현저영향가소화담(P=0.034 4)화담표관소화솔(P=0.043 6),기중10화20 mg/kg조식입담겁현저고우기타조(P<0.01) ,가소화담화담표관소화솔균재10 mg/kg조체도최대치,단여20 mg/kg조차이불현저(P>0.05). 사량유생소B6 첨가수평겁현저영향단안산적표관소화솔(P=0.006 8),현저영향반광안산(P=0.011 3)、곡안산(P=0.022 6)화사안산적표관소화솔(P=0.047 8),병균재20 mg/kg조체도최대치. 사량유생소B6 첨가수평현저영향이선이단백매( P=0.035 4)화미단백매활성( P=0.043 2) ,병균재20 mg/kg조체도최고치. 사량유생소B6 첨가수평현저영향혈청총단백(P=0.014 3)화뇨소담함량(P=0.048 2),병분별재20 mg/kg조체도최고치화최저치. 사량유생소B6 첨가수평겁현저영향혈청총항양화능력( P=0.004 5) ,현저영향혈청곡광감태과양화물매( P=0.044 2)화총초양화물기화매활성( P=0.048 1) ,병균재20 mg/kg조체도최고치,차혈청병이철함량재20 mg/kg조체도최저치. 종합본시험측정지표,3~5월령생장달토사량괄의적유생소B6 첨가수평위10~20 mg/kg.
The experiment was conducted to investigate the effects of dietary vitamin B6 supplemental level on digestion and metabolism, and antioxidant ability of 3 to 5-month old growing Rex rabbits. A total 200 3-month old health Rex rabbits were randomly divided into 5 groups with 40 replicates in each group and 1 rabbit in each replicate. Rabbits in the 5 groups were fed the basal diet supplemented with 0, 5, 10, 20 and 40 mg/kg vita-min B6, respectively. The trial lasted for 7 days for adaptation, and 54 days for test. The results showed as fol-lows: dietary vitamin B6 supplemental level had an extremely significant influence on nitrogen intake ( IN ) ( P=0.004 5) , and had significant influences on digestible nitrogen ( DN) ( P=0.034 4) and nitrogen appar-ent digestibility ( NAD) ( P=0.043 6) . The IN in 10 and 20 mg/kg groups was extremely significantly higher than that in other groups ( P<0.01) , and the DN and NAD in 10 mg/kg group came to the maximum, but had no significant influences compared with 20 mg/kg group ( P>0.05) . Dietary vitamin B6 supplemental level had an extremely significant influence on methionine apparent digestibility ( P=0.006 8) , had significant influence on cystine ( P=0.011 3) , glutamate ( P=0.022 6) and serine apparent digestibility ( P=0.047 8) , and all of them in 20 mg/kg group came to the maximum. Dietary vitamin B6 supplemental level had significant influ-ences on pancreatic trypsin (P=0.035 4) and chymotrypsin activities (P=0.043 2), and the highest values were all found in the 20 mg/kg group. Dietary vitamin B6 supplemental level had significant influences on ser-um total protein ( TP) ( P=0.014 3) and urea nitrogen ( UN) contents ( P=0.048 2) , and the highest value of TP content and the lowest value of UN content were all found in the 20 mg/kg group. Dietary vitamin B6 supplemental level had an extremely significant influence on serum total antioxidant capacity ( T-AOC ) ( P=0.006 9) , had significant influences on serum glutathione peroxidase ( GSH-Px) ( P=0.044 2) and total su-peroxide dismutase ( T-SOD ) activities ( P = 0. 048 1 ) , and the highest values of them were all in the 20 mg/kg group, meanwhile, the serum malonaldehyde ( MDA) content in the 20 mg/kg group had the mini-mum. Considering all indices of this experiment, the appropriate dietary vitamin B6 supplemental level is 10 to 20 mg/kg for 3 to 5-month old growing Rex rabbits.