郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
Journal of Zhengzhou University (Medical Sciences)
2015年
6期
798-800
,共3页
李永红%周方方%付路平%刘宏民
李永紅%週方方%付路平%劉宏民
리영홍%주방방%부로평%류굉민
利斯的明%手性药物中间体%ee值
利斯的明%手性藥物中間體%ee值
리사적명%수성약물중간체%ee치
rivastigmine%chiral drug intermediate%ee value
目的:建立RP-HPLC法测定生物转化液中利斯的明中间体含量及ee值的方法。方法:以Lux 3u Cellu-lose-1为手性分离柱,以正己烷砄异丙醇(V砄V=92.5砄7.5)为流动相,检测波长217 nm,进样量20μL。结果:中间体能够完全分离,在测定中间体含量的同时能够测定样品的ee值。结论:该方法准确、灵敏、精密度良好,适合生物转化液中利斯的明中间体含量及ee值的测定。
目的:建立RP-HPLC法測定生物轉化液中利斯的明中間體含量及ee值的方法。方法:以Lux 3u Cellu-lose-1為手性分離柱,以正己烷砄異丙醇(V砄V=92.5砄7.5)為流動相,檢測波長217 nm,進樣量20μL。結果:中間體能夠完全分離,在測定中間體含量的同時能夠測定樣品的ee值。結論:該方法準確、靈敏、精密度良好,適閤生物轉化液中利斯的明中間體含量及ee值的測定。
목적:건립RP-HPLC법측정생물전화액중리사적명중간체함량급ee치적방법。방법:이Lux 3u Cellu-lose-1위수성분리주,이정기완결이병순(V결V=92.5결7.5)위류동상,검측파장217 nm,진양량20μL。결과:중간체능구완전분리,재측정중간체함량적동시능구측정양품적ee치。결론:해방법준학、령민、정밀도량호,괄합생물전화액중리사적명중간체함량급ee치적측정。
Aim:To establish a RP-HPLC method to determine the ee values and content of rivastigmine intermediates in bioconversion broth.Methods:The determination was performed on a Lux 3u Cellulose-1 column with a 92.5:7.5(V:V) mixture of n-hexane and isopropanol alcohol as mobile phase , determination wavelength of 217 nm and injection volume of 20 μL.Results:The two intermediates were completely separated .The ee value could be determined as well as the de-termination of the content of the intermediates .Conclusion:The method is accurate and sensitive with high precision .It is suitable for the determination of the ee values and content of rivastigmine intermediates in bioconversion broth .