CAJ | 학술논문

目的：探讨宫颈鳞癌SiHa细胞中缺氧诱导因子-1α基因(HIF-1α)与抗凋亡基因生存素survivin基因的关系。方法本研究通过培养宫颈鳞癌SiHa细胞用氯化钴模拟缺氧以促进HIF-1a表达,再以RNA干扰技术抑制HIF-1a基因表达,再通过Westernblot法检测其中HIF-1a基因和survivin基因在乏氧状态下的表达。结果氯化钴培养后宫颈鳞癌SiHa细胞的HIF-1a蛋白表达增加,survivin蛋白表达也增加,并与使用的氯化钴的浓度有关。通过RNA干扰技术抑制HIF-1a基因的干扰后的细胞(SiHa/shRNA)中HIF-1a蛋白表达减少,survivin蛋白表达同时减少。结论在宫颈鳞癌SiHa细胞中, HIF-1α基因和survivin表达具有高度的相关性,并且抑制HIF-1α表达可能通过抑制survivin而促进宫颈鳞癌细胞的凋亡。
목적：탐토궁경린암SiHa세포중결양유도인자-1α기인(HIF-1α)여항조망기인생존소survivin기인적관계。방법본연구통과배양궁경린암SiHa세포용록화고모의결양이촉진HIF-1a표체,재이RNA간우기술억제HIF-1a기인표체,재통과Westernblot법검측기중HIF-1a기인화survivin기인재핍양상태하적표체。결과록화고배양후궁경린암SiHa세포적HIF-1a단백표체증가,survivin단백표체야증가,병여사용적록화고적농도유관。통과RNA간우기술억제HIF-1a기인적간우후적세포(SiHa/shRNA)중HIF-1a단백표체감소,survivin단백표체동시감소。결론재궁경린암SiHa세포중, HIF-1α기인화survivin표체구유고도적상관성,병차억제HIF-1α표체가능통과억제survivin이촉진궁경린암세포적조망。
Objective Discussion SiHa cervical squamous carcinoma cel s HIF -1α gene (HIF-1α) relationship with survivin's anti-apoptotic gene survivin gene. Methods This study through with cobalt chloride simulated hypoxia to promote the expression of HIF-1a in cultured cervical squamous cel carcinoma cel line SiHa, again by RNA interference inhibits the expression of HIF-1a gene, by Western blot method was used to detect the HIF-1a gene and survivin gene in the lack of oxygen condition expression. Results After incubation under hypoxic state and increased expression of HIF-1αin cervical squamous cel carcinoma SiHa cel s, survivin protein expression was also increased, and with the lack of oxygen concentration dependent;RNA interference to reduce the expression method of inhibiting HIF-1α gene in cel s HIF-1α , survivin protein expression is reduced. Conclusion In squamous cel carcinoma SiHa cel s, the gene expression of HIF-1α and survivin highly relevant, inhibition of HIF-1αexpression of survivin by inhibition of apoptosis may promote tumor cel s.