CAJ | 학술논문

利用反相-高效液相色谱法(RP-HPLC)建立了快速测定人参皂苷Re和Rh2含量方法。色谱柱为Agilent Extend C18（4.6×150 mm，5μm），流动相为乙腈-0.05%乙酸梯度洗脱溶液，流速1.00 mL/min，波长203 nm，柱温30.0℃。人参皂苷Re和Rh2的线性范围分别为0.17~456.6 mg/L和6.00~449.4 mg/L；人参皂苷Re和人参皂苷Rh2的平均加标回收率分别为99.60%和99.78%。本方法快速、灵敏、准确性好，可用于测定人参和西洋参提取物中人参皂苷Re和人参皂苷Rh2的含量。
이용반상-고효액상색보법(RP-HPLC)건립료쾌속측정인삼조감Re화Rh2함량방법。색보주위Agilent Extend C18（4.6×150 mm，5μm），류동상위을정-0.05%을산제도세탈용액，류속1.00 mL/min，파장203 nm，주온30.0℃。인삼조감Re화Rh2적선성범위분별위0.17~456.6 mg/L화6.00~449.4 mg/L；인삼조감Re화인삼조감Rh2적평균가표회수솔분별위99.60%화99.78%。본방법쾌속、령민、준학성호，가용우측정인삼화서양삼제취물중인삼조감Re화인삼조감Rh2적함량。
A method for determination ginsedoside Re and Rh2 has been developed by RP-HPLC. Agilent Ex-tend C18(4.6 mm × 150 mm,5 μm)served as the stationary phase. The mobile phase consists of actonitrile and 0.05% acetic acid in term of gradient operation. Detection wavelength was 203 nm,column temperature 30.0℃,flow rate,1.00 mL/min. The analytical linearity range of ginsenoside Re and Rh2 were 0.17~456.6 mg/L and 6.00~449.4 mg/L,respectively .The recoveries of ginsenoside Re and Rh2 were 99.60% and 99.78%,respectively. The method developed is rapid,simple and accurate and can be used to determine the ginsenoside Re and Rh2 of Radix et Rhizoma Ginseng and Panax quinquefolium L extraction product.