CAJ | 학술논문

目的：观察重复经颅磁刺激（rTMS）对抑郁大鼠学习记忆功能及海马CA3区突触素（SYP）表达的影响。方法：SD雄性大鼠40只，随机分为对照组、抑郁组、rTMS组和伪刺激组，每组10只，后3组采用为期21 d的孤养结合慢性不可预见轻度应激制备抑郁模型。造模成功后，rTMS组给予3个疗程15 Hz的rTMS治疗；伪刺激组只施予同样次数的声音刺激而无电流脉冲。采用Morris水迷宫的定位航行实验及空间探索实验评定大鼠学习记忆功能。应用免疫组织化学方法检测大鼠海马CA3区SYP蛋白的表达情况。结果：4组大鼠逃避潜伏期、原平台象限游泳时间及原平台象限游泳路程占总路程的百分比及海马CA3区SYP蛋白表达水平比较，差异均有统计学意义（ F＝39．608、646．088、198．940、388．673，P＜0．001）。与对照组比较，抑郁组、伪刺激组大鼠逃避潜伏期延长，原平台象限游泳时间缩短，原平台象限游泳路程占总路程的百分比减小，SYP蛋白表达水平降低（P＜0．05）；与抑郁组、伪刺激组比较，rTMS组上述指标均明显改善（P＜0．05）。结论：rTMS干预可以改善抑郁模型大鼠的学习记忆功能，其机制可能与rTMS增加大鼠海马CA3区SYP的表达有关。
목적：관찰중복경로자자격（rTMS）대억욱대서학습기억공능급해마CA3구돌촉소（SYP）표체적영향。방법：SD웅성대서40지，수궤분위대조조、억욱조、rTMS조화위자격조，매조10지，후3조채용위기21 d적고양결합만성불가예견경도응격제비억욱모형。조모성공후，rTMS조급여3개료정15 Hz적rTMS치료；위자격조지시여동양차수적성음자격이무전류맥충。채용Morris수미궁적정위항행실험급공간탐색실험평정대서학습기억공능。응용면역조직화학방법검측대서해마CA3구SYP단백적표체정황。결과：4조대서도피잠복기、원평태상한유영시간급원평태상한유영로정점총로정적백분비급해마CA3구SYP단백표체수평비교，차이균유통계학의의（ F＝39．608、646．088、198．940、388．673，P＜0．001）。여대조조비교，억욱조、위자격조대서도피잠복기연장，원평태상한유영시간축단，원평태상한유영로정점총로정적백분비감소，SYP단백표체수평강저（P＜0．05）；여억욱조、위자격조비교，rTMS조상술지표균명현개선（P＜0．05）。결론：rTMS간예가이개선억욱모형대서적학습기억공능，기궤제가능여rTMS증가대서해마CA3구SYP적표체유관。
Aim:To investigate the effect of repetitive transcranial magnetic stimulation ( rTMS) on learning and mem-ory abilities, the expression of SYP protein in hippocampus CA3 region of depressed rats.Methods: Forty SD rats were randomly allocated into 4 groups:control group, depression group, rTMS group,and fake stimulation group,10 in each group.Rats in the latter 3 groups were given chronic unpredictable mild stress to establish depression model .rTMS group rats were given 15 Hz rTMS for 3 courses .Sham stimulation group were given only the same number of sound stimulation without electricity and current pulse .Then, Morris water maze test, including place navigation and spatial probe test , were performed to access the rats′learning and memory abilities , and the expression of SYP protein in hippocampus CA 3 was de-tected by immunohistochemistry .Results:There were significant differences in the escape latency ,the swimming time in o-riginal platform quadrant , the swimming distance percentage of total swimming distance in the original platform quadrant , and the expression of SYP protein in hippocampus CA 3 region among the 4 groups ( F =39.608,646.088,198.940, 388.673,P<0.001).Compared with the control group , the escape latency prolonged , the swimming time in original plat-form quadrant shortened , the swimming distance percentage of total swimming distance in the original platform quadrant re -duced, and the expression of SYP protein decreased in depression group and fake stimulation group (P<0.05).Compared with the depression group and fake stimulation group , the indexes mentioned above were improved ( P<0.05 ) in rTMS group.Conclusion:rTMS can improve the learning and memory abilities of depressed rats , the possible mechanism is in-creasing the expression of SYP in hippocampal CA 3 region.