CAJ | 학술논문

本试验旨在研究饲粮中添加不同种类的酸化剂对肉鸡肠道发育、消化酶活性以及微生物数量的影响. 试验选用1日龄的爱拔益加肉公鸡840只,随机分为7个处理,每个处理6个重复,每个重复20只鸡. 对照组饲喂基础饲粮,其余6 组饲喂在基础饲粮中分别添加吸附磷酸( PA)、微囊化磷酸( CPA)、吸附磷酸乳酸( PLA)、富马酸( FUA)、甲酸( FA)和柠檬酸( CA)的试验饲粮,氢离子( H+)浓度均为9.18 mmol/kg. 试验期为42 d. 结果表明:1)饲粮中添加PA显著提高了42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性( P<0.05). 2)饲粮中添加CPA显著增加了21和42日龄肉鸡小肠糜蛋白酶活性( P<0.05). 3)饲粮中添加PLA显著提高了42日龄肉鸡小肠胰蛋白酶活性( P<0.05). 4)饲粮中添加FUA显著增加了42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性( P<0.05) ,显著降低了42日龄肉鸡盲肠大肠杆菌数量( P<0.05). 5)饲粮中添加FA显著增加了21和42日龄肉鸡小肠胰蛋白酶活性( P<0.05) ,显著降低了42日龄肉鸡盲肠大肠杆菌数量( P<0.05). 6)饲粮中添加CA显著增加了21和42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性(P<0.05),显著降低了42日龄肉鸡盲肠大肠杆菌数量(P<0.05). 由此可见,PA、CPA和PLA均可不同程度增强肉鸡小肠消化酶活性,而FUA、FA和CA则均具有改善肉鸡肠道消化酶活性和降低有害微生物数量的功能.
본시험지재연구사량중첨가불동충류적산화제대육계장도발육、소화매활성이급미생물수량적영향. 시험선용1일령적애발익가육공계840지,수궤분위7개처리,매개처리6개중복,매개중복20지계. 대조조사위기출사량,기여6 조사위재기출사량중분별첨가흡부린산( PA)、미낭화린산( CPA)、흡부린산유산( PLA)、부마산( FUA)、갑산( FA)화저몽산( CA)적시험사량,경리자( H+)농도균위9.18 mmol/kg. 시험기위42 d. 결과표명:1)사량중첨가PA현저제고료42일령육계소장이단백매화미단백매활성( P<0.05). 2)사량중첨가CPA현저증가료21화42일령육계소장미단백매활성( P<0.05). 3)사량중첨가PLA현저제고료42일령육계소장이단백매활성( P<0.05). 4)사량중첨가FUA현저증가료42일령육계소장이단백매화미단백매활성( P<0.05) ,현저강저료42일령육계맹장대장간균수량( P<0.05). 5)사량중첨가FA현저증가료21화42일령육계소장이단백매활성( P<0.05) ,현저강저료42일령육계맹장대장간균수량( P<0.05). 6)사량중첨가CA현저증가료21화42일령육계소장이단백매화미단백매활성(P<0.05),현저강저료42일령육계맹장대장간균수량(P<0.05). 유차가견,PA、CPA화PLA균가불동정도증강육계소장소화매활성,이FUA、FA화CA칙균구유개선육계장도소화매활성화강저유해미생물수량적공능.
To investigate the effects of different kinds of acidifier on intestinal development, digestive enzyme activities and microbiota number of broiler chickens, a total of 840 one-day-old male Arbor Acres ( AA) broil-er chicks were randomly allocated into 7 treatments with 6 replicates per group and 20 broilers per replicate. The control group was fed a basal diet, and test groups were fed the basal diet supplemented with phosphoric acid ( PA) , coated phosphoric acid ( CPA) , phosphoric acid+lactic acid ( PLA) , fumaric acid ( FUA) , for-mic acid ( FA) and citric acid ( CA) , respectively ( the H+concentration was 9.18 mmol/kg) . The experiment lasted for 42 days. The results showed as follows:1) dietary supplemented with PA significantly increased the activities of trypsin and chymotrypsin in small intestine at 21 and 42 days of age ( P<0.05) . 2) Dietary supple-mented with CPA significantly increased the chymotrypsin activity in small intestine (P<0.05). 3) Dietary supplemented with PLA significantly increased trypsin activity in small intestine at 42 days of age ( P<0.05) . 4) Dietary supplemented with FUA significantly increased the activities of trypsin and chymotrypsin in the small intestine at 42 days of age ( P<0.05) , but significantly reduced the number of Escherichia coli in cecal at 42 days of age ( P<0.05) . 5) Dietary supplemented with FA significantly increased the trypsin activity in the small intestine at 21 and 42 days of age ( P<0.05) , but significantly reduced the number of Escherichia coli in cecal at 42 days of age (P<0.05). 6) Dietary supplemented with CA significantly increased the activities of trypsin and chymotrypsin in the small intestine at 21 and 42 days of age ( P<0.05) , but significantly reduced the number of Escherichia coli in cecal at 42 days of age (P<0.05). In conclusion, PA, CPA and PLA can increase small intestinal digestive enzyme activities; FUA, FA and CA can improve small intestinal digestive enzyme activities, and decrease harmful cecal microbiota number of broilers.