郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
Journal of Zhengzhou University (Medical Sciences)
2015年
6期
740-744
,共5页
于超男%王颖%燕树勋%杨国杰%张彦周%杨晓村%李渊%孙丽娜
于超男%王穎%燕樹勛%楊國傑%張彥週%楊曉村%李淵%孫麗娜
우초남%왕영%연수훈%양국걸%장언주%양효촌%리연%손려나
高血压%睾酮受体%血管重构%丝裂原活化蛋白激酶%磷酸酶
高血壓%睪酮受體%血管重構%絲裂原活化蛋白激酶%燐痠酶
고혈압%고동수체%혈관중구%사렬원활화단백격매%린산매
hypertension%testosterone receptor%vascular remodeling%mitogen-activated protein kinase%phosphatase
目的：探讨睾酮受体（ AR）蛋白表达水平的改变对高血压大鼠血管重构（ VR）的影响及其信号转导机制。方法：将30只大鼠随机分为对照组、假手术组、高血压组3组（每组10只）。采用“两肾一夹”法制备肾血管性高血压大鼠模型。 HE染色观察主动脉组织形态结构，免疫组化法检测3组大鼠主动脉组织中AR、丝裂原活化蛋白激酶磷酸酶-1（MKP-1）蛋白表达水平。结果：与对照组相比，假手术组主动脉形态结构基本正常，AR、MKP-1蛋白表达水平无显著改变（P＞0．05）；与对照组及假手术组相比，AR和MKP-1蛋白表达水平下降（P＜0．001）；且在高血压组AR与MKP-1蛋白的表达呈正相关（r＝0．657，P＝0．039），在对照组及假手术组无关（r＝0．589和0．436， P＞0．05）。结论：高血压VR过程中存在AR及MKP-1蛋白表达水平的改变。
目的：探討睪酮受體（ AR）蛋白錶達水平的改變對高血壓大鼠血管重構（ VR）的影響及其信號轉導機製。方法：將30隻大鼠隨機分為對照組、假手術組、高血壓組3組（每組10隻）。採用“兩腎一夾”法製備腎血管性高血壓大鼠模型。 HE染色觀察主動脈組織形態結構，免疫組化法檢測3組大鼠主動脈組織中AR、絲裂原活化蛋白激酶燐痠酶-1（MKP-1）蛋白錶達水平。結果：與對照組相比，假手術組主動脈形態結構基本正常，AR、MKP-1蛋白錶達水平無顯著改變（P＞0．05）；與對照組及假手術組相比，AR和MKP-1蛋白錶達水平下降（P＜0．001）；且在高血壓組AR與MKP-1蛋白的錶達呈正相關（r＝0．657，P＝0．039），在對照組及假手術組無關（r＝0．589和0．436， P＞0．05）。結論：高血壓VR過程中存在AR及MKP-1蛋白錶達水平的改變。
목적：탐토고동수체（ AR）단백표체수평적개변대고혈압대서혈관중구（ VR）적영향급기신호전도궤제。방법：장30지대서수궤분위대조조、가수술조、고혈압조3조（매조10지）。채용“량신일협”법제비신혈관성고혈압대서모형。 HE염색관찰주동맥조직형태결구，면역조화법검측3조대서주동맥조직중AR、사렬원활화단백격매린산매-1（MKP-1）단백표체수평。결과：여대조조상비，가수술조주동맥형태결구기본정상，AR、MKP-1단백표체수평무현저개변（P＞0．05）；여대조조급가수술조상비，AR화MKP-1단백표체수평하강（P＜0．001）；차재고혈압조AR여MKP-1단백적표체정정상관（r＝0．657，P＝0．039），재대조조급가수술조무관（r＝0．589화0．436， P＞0．05）。결론：고혈압VR과정중존재AR급MKP-1단백표체수평적개변。
Aim:To explore the effect of the change of androgen receptor ( AR) protein expression level on hypertensive vascular remodeling and the underlying mechanisms .Methods:Thirty rats were randomly allocated into 3 groups:control group, sham operation group and hypertensive group (10 rats in each group).The renovascular hypertensive rats model was established using the "2-kidney and 1-clip"method.Using tail-cuff method to measure the systolic blood pressure (pSB) and HE staining to observe the morphological structure of aorta , and the expressions of AR and MKP-1 were detected by immuno-histochemical technique .Results:For the morphological structure , there was no significant difference between the control group and the sham operation group , and also no significant difference in the expressions of AR and MKP-1 was found be-tween the two groups(P>0.05).Compared with the control group and the sham operation group ,the vascular remodeling of the aorta in the hypertensive group was remarkable , and the expressions of AR and MKP-1 significantly decreased ( P<0.001).In the hypertensive group, the expression of AR was positively correlated with the expression of MKP -1(r=0.657, P=0.039), and no such correlation was found in the control group or in the sham operation group (r=0.589,0.436,P>0.05).Conclusion:The vascular remodeling during hypertension is accompanied by the down-regulation of AR and MKP-1.
