CAJ | 학술논문

目的：研究枸杞多糖及其硫酸酯体外免疫和抗肿瘤活性。方法：分别采用水提一步醇沉法和分步醇沉法提取不同的多糖LBPS30、LBPS70和LBPSt，纯化后用氯磺酸-吡啶法修饰得到3种硫酸化枸杞多糖sLBPS30、sLB-PS70和sLBPSt。采用MTT法检测多糖对小鼠脾淋巴细胞增殖和食管癌EC109细胞抑制的影响，ELISA法检测细胞上清液中TNF-α、IL-2、IFN-γ和IL-24的含量，划痕法检测细胞迁移情况。结果：sLBPSt和 sLBPS30的淋巴细胞增殖率和不同时间点对EC109细胞的抑制率都较高； sLBPSt组细胞上清液中4种淋巴细胞因子含量均显著高于细胞对照组（P＜0．05）；sLBPSt组细胞迁移数显著低于其余各组（P＜0．05）。结论：硫酸化修饰能显著增强体外脾淋巴细胞增殖，促进淋巴细胞四种细胞因子的分泌，并能抑制EC109细胞生长，降低EC109细胞迁移速率，且与硫酸基含量有一定的关系。 sLBPSt和sLBPS30总体效果较好。
목적：연구구기다당급기류산지체외면역화항종류활성。방법：분별채용수제일보순침법화분보순침법제취불동적다당LBPS30、LBPS70화LBPSt，순화후용록광산-필정법수식득도3충류산화구기다당sLBPS30、sLB-PS70화sLBPSt。채용MTT법검측다당대소서비림파세포증식화식관암EC109세포억제적영향，ELISA법검측세포상청액중TNF-α、IL-2、IFN-γ화IL-24적함량，화흔법검측세포천이정황。결과：sLBPSt화 sLBPS30적림파세포증식솔화불동시간점대EC109세포적억제솔도교고； sLBPSt조세포상청액중4충림파세포인자함량균현저고우세포대조조（P＜0．05）；sLBPSt조세포천이수현저저우기여각조（P＜0．05）。결론：류산화수식능현저증강체외비림파세포증식，촉진림파세포사충세포인자적분비，병능억제EC109세포생장，강저EC109세포천이속솔，차여류산기함량유일정적관계。 sLBPSt화sLBPS30총체효과교호。
Aim: To screen the effective Lycium barbarum polysaccharides ( LBPSs ) according to researching the effects of immunity and antitumor activity on LBPSs and their sulfates in vitro .Methods:Three types of LBPSs , LBPS30 ,LBPS70 and LBPSt were extracted by water decoction and stepwise or one-step ethanol precipitation methods and further modified using the chlorosulfonic acid-pyridine method based on previous experiments , yielding three sulfated LBPSs ( sLB-PSs), sLBPSt, sLBPS30, sLBPS70, respectively.The effects of six polysaccharides spleen lymphocyte proliferation and their antitumor activities against EC109 cells in vitro were evaluated by MTT method .The contents of TNF-α, IL-2, IFN-γand IL-24 in cell supernatant were determined by ELISA method .Cell migration model was induced by scratch method and the migrating cells were counted to observe the effects of polysaccharides on EC 109 cell migration inhibition .Results:The lymphocyte proliferation ratios and inhibition ratio against EC 109 of sLBPSt and sLBPS30 group at different time points were higher than those of other groups , the contents of four cytokines of sLBPSt group were significantly higher than those of the control group(P<0.05), the migrating cells of sLBPSt group were also fewer than those of other group (P<0.05). Conclusion:sLBPSs could increase conA-activated lymphopoiesis , promote the secretion of TNF-α, IL-2, IFN-γand IL-24.Moreover, they could inhibit the EC109 cells′viability and reduce the rates of tumor cell migration in vitro , indica-ting that sulfated modification enhanced the immunoregulatory and antitumor activities of LBPSs .sLBPS30 and sLBPSt showed the best performance , could be used as materials for further research .