中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
Chinese Journal of Clinical Nutrition
2015年
5期
292-295
,共4页
肠屏障损伤%细菌移位%发热%肠道菌%血培养%实时定量PCR
腸屏障損傷%細菌移位%髮熱%腸道菌%血培養%實時定量PCR
장병장손상%세균이위%발열%장도균%혈배양%실시정량PCR
Gut barrier injuries%Bacterial translocation%Fever%Enterobacteria%Blood culture%Real-time quantitative polymerase chain reaction
目的 采用实时定量PCR (RQ-PCR)检测外科发热患者静脉血中肠道DNA,研究其与体征及血细胞计数之间的相关性,并比较不同检测方法对血中细菌阳性检出率的差异.方法 对72份血标本进行常规细菌培养及RQ-PCR定量检测,比较两种检测方法对血中细菌阳性检出率的差异,并计算细菌DNA含量与体温、心率、白细胞计数、中性粒细胞及淋巴细胞百分比之间的相关性.结果 RQ-PCR定量检测细菌DNA阳性率(63.89％)显著高于细菌培养阳性率(9.72％) (F=4.383,P=0.036).血中细菌DNA含量与体温和心率显著相关(P =0.006,r=0.323;P=0.000,r =0.411),与白细胞计数、中性粒细胞及淋巴细胞百分比无相关性(P=0.438,r=0.093;P=0.825,r=0.027;P=0.451,r=-0.090);同年龄无相关性(P =0.096,r=0.198)结论 RQ-PCR可用于定量检测外周血中细菌DNA的含量,快速且灵敏度高.血细胞计数不能较好地反映血中细菌的含量,而体温、心率的影响因素较多.
目的 採用實時定量PCR (RQ-PCR)檢測外科髮熱患者靜脈血中腸道DNA,研究其與體徵及血細胞計數之間的相關性,併比較不同檢測方法對血中細菌暘性檢齣率的差異.方法 對72份血標本進行常規細菌培養及RQ-PCR定量檢測,比較兩種檢測方法對血中細菌暘性檢齣率的差異,併計算細菌DNA含量與體溫、心率、白細胞計數、中性粒細胞及淋巴細胞百分比之間的相關性.結果 RQ-PCR定量檢測細菌DNA暘性率(63.89％)顯著高于細菌培養暘性率(9.72％) (F=4.383,P=0.036).血中細菌DNA含量與體溫和心率顯著相關(P =0.006,r=0.323;P=0.000,r =0.411),與白細胞計數、中性粒細胞及淋巴細胞百分比無相關性(P=0.438,r=0.093;P=0.825,r=0.027;P=0.451,r=-0.090);同年齡無相關性(P =0.096,r=0.198)結論 RQ-PCR可用于定量檢測外週血中細菌DNA的含量,快速且靈敏度高.血細胞計數不能較好地反映血中細菌的含量,而體溫、心率的影響因素較多.
목적 채용실시정량PCR (RQ-PCR)검측외과발열환자정맥혈중장도DNA,연구기여체정급혈세포계수지간적상관성,병비교불동검측방법대혈중세균양성검출솔적차이.방법 대72빈혈표본진행상규세균배양급RQ-PCR정량검측,비교량충검측방법대혈중세균양성검출솔적차이,병계산세균DNA함량여체온、심솔、백세포계수、중성립세포급림파세포백분비지간적상관성.결과 RQ-PCR정량검측세균DNA양성솔(63.89％)현저고우세균배양양성솔(9.72％) (F=4.383,P=0.036).혈중세균DNA함량여체온화심솔현저상관(P =0.006,r=0.323;P=0.000,r =0.411),여백세포계수、중성립세포급림파세포백분비무상관성(P=0.438,r=0.093;P=0.825,r=0.027;P=0.451,r=-0.090);동년령무상관성(P =0.096,r=0.198)결론 RQ-PCR가용우정량검측외주혈중세균DNA적함량,쾌속차령민도고.혈세포계수불능교호지반영혈중세균적함량,이체온、심솔적영향인소교다.
Objective To determine enterobacteria DNA load in venous blood of febrile surgical patients using real-time quantitative polymerase chain reaction (RQ-PCR), to study the correlations between DNA load and vital signs/blood cell count, and to compare the difference between different detection methods in terms of positive rates.Methods A total of 72 blood samples were obtained for bacterial cuhure and RQ-PCR.The correlations of enterobacteria DNA load with body temperature, heart rate, while blood cell count,and percentages of leukocyte and lymphocyte were then analyzed.Results The enterobacteria positive rate determined by RQ-PCR (63.89％) was significantly higher than that by bacterial culture (9.72％) (F =4.383, P =0.036).The DNA load was significantly correlated with both body temperature and heart rate (P =0.006, r =0.323;P =0.000, r =0.411), but not with white blood cell count, percentages of leukocyte and lymphocyte, and age (P=0.438, r=0.093;P=0.825, r=0.027;P=0.451, r=-0.090;P =0.096, r =0.198).Conclusions RQ-PCR can quickly determine the enterobacteria DNA load in peripheral blood with high sensitivity.Routine blood cell count may not accurately reflect the enterobacteria DNA load in blood.Body temperature and heart rate may be influenced by various factors.
