CAJ | 학술논문

目的应用基于离体高分辨磁共振波谱技术(ex-vivo high-resolution 1H-magnetic resonance spectroscopy,1H-MRS)的代谢组学技术分析新生大鼠在单次持续低血糖处理后新生期和成年期顶叶的代谢变化,探讨单次持续低血糖所致的顶叶损伤的可能发生机制. 方法将7日龄健康足月Sprague-Dawley大鼠随机分为低血糖组与对照组,每组20只.2组大鼠先禁食2h,之后低血糖组腹腔注射胰岛素40 U/kg诱导低血糖,对照组给予等剂量生理盐水.造模成功后,分别于新生期(8 d)和成年期(60d)分批进行大鼠顶叶脑组织采集和离体磁共振波谱扫描.采用独立样本t检验进行统计学分析. 结果 (1)低血糖组胰岛素注射后1h血糖为(2.24±0.35)mmol/L,4和7h时血糖均＜1.1 mmol/L(低于血糖仪检测下限).腹腔注射葡萄糖0.5 h后,血糖值为(4.90±1.20) mmol/L,恢复正常范围.对照组注射生理盐水后血糖均在正常范围波动.(2)新生期低血糖组和对照组在第一主成分上基本能够区分开,表明两者的代谢模式存在差异,牛磺酸、乳酸和丙氨酸等代谢物对2组的区分贡献较大.成年期低血糖组和对照组在第一主成分上基本能够区分开,表明两者的代谢模式存在差异,N-乙酰天门冬氨酸、γ-氨基丁酸和胆碱对2组的区分贡献较大.在新生期,低血糖组谷氨酰胺、谷氨酸、牛磺酸和琥珀酸含量较对照组明显升高,而γ-氨基丁酸、乳酸和丙氨酸含量明显降低.在成年期,低血糖组γ-氨基丁酸、天冬氨酸、乳酸和牛磺酸含量较对照组明显升高,而N-乙酰天门冬氨酸和胆碱含量明显降低. 结论新生大鼠单次持续低血糖可引起新生期大鼠顶叶明显的代谢紊乱,到成年期代谢紊乱持续存在.这可能与该区域神经元损伤持续存在及代偿修复相关.
목적 응용기우리체고분변자공진파보기술(ex-vivo high-resolution 1H-magnetic resonance spectroscopy,1H-MRS)적대사조학기술분석신생대서재단차지속저혈당처리후신생기화성년기정협적대사변화,탐토단차지속저혈당소치적정협손상적가능발생궤제. 방법 장7일령건강족월Sprague-Dawley대서수궤분위저혈당조여대조조,매조20지.2조대서선금식2h,지후저혈당조복강주사이도소40 U/kg유도저혈당,대조조급여등제량생리염수.조모성공후,분별우신생기(8 d)화성년기(60d)분비진행대서정협뇌조직채집화리체자공진파보소묘.채용독립양본t검험진행통계학분석. 결과 (1)저혈당조이도소주사후1h혈당위(2.24±0.35)mmol/L,4화7h시혈당균＜1.1 mmol/L(저우혈당의검측하한).복강주사포도당0.5 h후,혈당치위(4.90±1.20) mmol/L,회복정상범위.대조조주사생리염수후혈당균재정상범위파동.(2)신생기저혈당조화대조조재제일주성분상기본능구구분개,표명량자적대사모식존재차이,우광산、유산화병안산등대사물대2조적구분공헌교대.성년기저혈당조화대조조재제일주성분상기본능구구분개,표명량자적대사모식존재차이,N-을선천문동안산、γ-안기정산화담감대2조적구분공헌교대.재신생기,저혈당조곡안선알、곡안산、우광산화호박산함량교대조조명현승고,이γ-안기정산、유산화병안산함량명현강저.재성년기,저혈당조γ-안기정산、천동안산、유산화우광산함량교대조조명현승고,이N-을선천문동안산화담감함량명현강저. 결론 신생대서단차지속저혈당가인기신생기대서정협명현적대사문란,도성년기대사문란지속존재.저가능여해구역신경원손상지속존재급대상수복상관.
Objective To analyze the metabolic changes in parietal cortex in different growth periods after single continuous hypoglycemia induction in neonatal rats with ex vivo high resolution 1H-magnetic resonance spectroscopy.Methods A total of 40 healthy term Sprague Dawley neonatal rats were randomly divided into experimental group (n=20) and control group (n-20) on postnatal days 7 (P7).All rats were fasted for 2 h and followed by, hypoglycemia induction with intraperitoneal injection of insulin (40 U/kg) in the experimental group, while the sham received the same dose of normal saline.All rats were randomly selected on P8 and P60 in each group and received magnetic resonance spectroscopy scan of the parietal cortex.Independent sample t-test was used for statistic analysis.Results In the experimental group, the mean glucose level was (2.24±0.35) mmol/L at one hour after insulin injection, and ＜ 1.1 mmol/L at four and seven hours after insulin injection.At 0.5 h after peritoneal injection of glucose, the glucose level returned to normal [(4.90 ± 1.20)mmol/L].In the control group, the glucose level kept normal during the experiment.Distinct and easily detectable differences between the two groups were found.Partial least squares-discriminant analysis (PLS-DA) showed satisfactory clustering between experimental and control group in neonatal and adult period, suggesting that the metabolic profiles of the two groups were markedly different.Compared to control group, rats in the experimental group during neonatal stage had higher levels of glutamine, glutamate, taurine and succinate, together with lower levels of gamma-amino-butyric acid, lactate and alanine.However, the experimental group had higher levels of gamma-amino-butyric acid, aspartate, alanine and taurine, together with lower levels of N-acetyl aspartate and choline than the control group in adult rats.Conclusions Single continuous hypoglycemia has obvious damage effects on parietal lobe of neonatal rat and this injury sustains to adult period, which might be associated with different adaptive and compensative changes at different stages.