中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
8期
948-950
,共3页
呼吸,人工%氦%氧%呼吸窘迫综合征,成人
呼吸,人工%氦%氧%呼吸窘迫綜閤徵,成人
호흡,인공%양%양%호흡군박종합정,성인
Respiration,artificial%Helium%Oxygen%Respiratory distress syndrome,adult
目的 评价氦氧机械通气对兔急性肺损伤的影响.方法 健康成年雄性新西兰大白兔30只,体重2.2～2.4 kg,采用随机数字表法分为3组(n=10):对照组(C组)、急性肺损伤组(ALI组)和氦氧机械通气组(HO组).ALI组和HO组右侧支气管内注入pH值为1.0的盐酸1.2 ml/kg,然后注入2 ml空气;5 min后向左侧支气管内注入pH值为1.0的盐酸0.8 ml/kg,然后注入2 ml空气,制备急性肺损伤模型,C组以同样方式向气管内注入等容量生理盐水.造模成功后,C组和ALI组行空氧机械通气(空气50％-氧气50％),HO组行氦氧机械通气(氦气50％-氧气50％),3组通气时间为4h,潮气量8 ml/kg,通气频率30次/min,吸呼比1:1.分别于注入盐酸前30 min(T0)、注入盐酸后30min(T1)、机械通气1、2、3和4 h(T2-5)时,取动脉血样,采用ELISA法测定血清肺表面活性蛋白A(SP-A)浓度.T5时点处死动物,取肺组织,光镜下观察病理学结果,计算湿重/干重(W/D)比值.结果 与C组比较,ALI组和HO组T1-5时血清SP-A浓度升高,肺组织W/D比值升高(P＜0.05或0.01);与ALI组比较,HO组T5时血清SP-A浓度降低,肺组织W/D比值降低(P＜0.05或0.01),病理学损伤减轻.结论 氦氧机械通气可减轻兔急性肺损伤.
目的 評價氦氧機械通氣對兔急性肺損傷的影響.方法 健康成年雄性新西蘭大白兔30隻,體重2.2～2.4 kg,採用隨機數字錶法分為3組(n=10):對照組(C組)、急性肺損傷組(ALI組)和氦氧機械通氣組(HO組).ALI組和HO組右側支氣管內註入pH值為1.0的鹽痠1.2 ml/kg,然後註入2 ml空氣;5 min後嚮左側支氣管內註入pH值為1.0的鹽痠0.8 ml/kg,然後註入2 ml空氣,製備急性肺損傷模型,C組以同樣方式嚮氣管內註入等容量生理鹽水.造模成功後,C組和ALI組行空氧機械通氣(空氣50％-氧氣50％),HO組行氦氧機械通氣(氦氣50％-氧氣50％),3組通氣時間為4h,潮氣量8 ml/kg,通氣頻率30次/min,吸呼比1:1.分彆于註入鹽痠前30 min(T0)、註入鹽痠後30min(T1)、機械通氣1、2、3和4 h(T2-5)時,取動脈血樣,採用ELISA法測定血清肺錶麵活性蛋白A(SP-A)濃度.T5時點處死動物,取肺組織,光鏡下觀察病理學結果,計算濕重/榦重(W/D)比值.結果 與C組比較,ALI組和HO組T1-5時血清SP-A濃度升高,肺組織W/D比值升高(P＜0.05或0.01);與ALI組比較,HO組T5時血清SP-A濃度降低,肺組織W/D比值降低(P＜0.05或0.01),病理學損傷減輕.結論 氦氧機械通氣可減輕兔急性肺損傷.
목적 평개양양궤계통기대토급성폐손상적영향.방법 건강성년웅성신서란대백토30지,체중2.2～2.4 kg,채용수궤수자표법분위3조(n=10):대조조(C조)、급성폐손상조(ALI조)화양양궤계통기조(HO조).ALI조화HO조우측지기관내주입pH치위1.0적염산1.2 ml/kg,연후주입2 ml공기;5 min후향좌측지기관내주입pH치위1.0적염산0.8 ml/kg,연후주입2 ml공기,제비급성폐손상모형,C조이동양방식향기관내주입등용량생리염수.조모성공후,C조화ALI조행공양궤계통기(공기50％-양기50％),HO조행양양궤계통기(양기50％-양기50％),3조통기시간위4h,조기량8 ml/kg,통기빈솔30차/min,흡호비1:1.분별우주입염산전30 min(T0)、주입염산후30min(T1)、궤계통기1、2、3화4 h(T2-5)시,취동맥혈양,채용ELISA법측정혈청폐표면활성단백A(SP-A)농도.T5시점처사동물,취폐조직,광경하관찰병이학결과,계산습중/간중(W/D)비치.결과 여C조비교,ALI조화HO조T1-5시혈청SP-A농도승고,폐조직W/D비치승고(P＜0.05혹0.01);여ALI조비교,HO조T5시혈청SP-A농도강저,폐조직W/D비치강저(P＜0.05혹0.01),병이학손상감경.결론 양양궤계통기가감경토급성폐손상.
Objective To evaluate the effects of mechanical ventilation with heliox-oxygen on acute lung injury (ALI) in rabbits.Methods Thirty healthy New Zealand male rabbits, weighing 2.2-2.4 kg, were randomly divided into 3 groups (n =10 each) using a random number table: control group (C group), ALI group and mechanical ventilation with helium-oxygen group (HO group).The right common carotid artery was cannulated for fluid administration and blood sampling in the rabbits anesthetized with urethane.In ALI and HO groups, 1.2 ml/kg hydrochloric acid (HC1) of pH value 1.0 was injected into the right bronchus, followed by 2 ml air injection, and 5 min later 0.8 ml/kg HCI of pH value 1.0 was injected into the left bronchus, followed by 2 ml air injection.In C group, the equal volume of normal saline was given as previously described.After ALI, the rabbits were mechanically ventilated with airoxygen (air 50％-oxygen 50％) in C and ALI groups, or with helium-oxygen (helium 50％-oxygen 50％)in HO group.Mechanical ventilation (tidal volume 8 ml/kg, respiratory rate 30 breaths/min, inspiratory/ expiratory ratio 1 : 1) lasted for 4 h in the three groups.At 30 min before and after HC1 injection (T0,1) and 1, 2, 3 and 4 h of ventilation (T2-T5) , arterial blood samples were collected to detect the levels of surfactant-protein (SP-A) in serum by enzyme-linked immunosorbent assay.The animals were sacrificed at T5, and lungs were removed for examination of the pathologic changes with light microscope.The wet to dry lung weight ratio (W/D ratio) was calculated.Results Compared with group C, the levels of SP-A in serum were significantly increased at T1-T5, and W/D ratio was increased in ALI and HO groups.Compared with group ALI, the levels of SP-A in serum were significantly decreased at T5, and W/D ratio was decreased in HO group.Conclusion Mechanical ventilation with heliox-oxygen can reduce ALI in rabbits.
