CAJ | 학술논문

目的评价全氟化碳不同给药时机对兔急性肺损伤(ALI)的影响.方法新西兰大白兔18只,体重1.8～ 2.4 kg,50～ 60日龄,雌雄不拘.采用随机数字表法,将其分为3组(n=6):ALI组、全氟化碳预防性给药组(PP组)和全氟化碳治疗性给药组(PA组).ALI组机械通气60 min时,于5 min内颈内静脉注射油酸0.1 ml/kg制备ALI模型,分3次注射完毕;PP组吸人全氟化碳2ml· kg-1·h-160 min,然后制备ALI模型;PA组机械通气60 min时制备ALI模型,模型制备成功后吸入全氟化碳2 ml· kg-1·h-160 min.分别于麻醉稳定30 min(T0)、机械通气60 min或全氟化碳预防性给药后60 min(T1)、造模后30 min(T2)、造模后60 min(T3)、造模后90 min(T4)和造模后150 min(T5)时,取动脉血样行血气分析,计算氧合指数.于T5时,收集支气管肺泡灌洗液(BALF),然后处死大鼠,取肺组织,采用ELISA法检测BALF中TNF-α和IL-1β的浓度,计算肺组织湿重/干重比值(W/D比值),采用Western blot法检测Toll样受体4(TLR4)和NF-κB p65的表达水平.结果与T0和T1时比较,3组T2-5时氧合指数均降低(P＜0.05);与ALI组比较,PP组T2-5时氧合指数升高,BALF中TNF-α和IL-1β的浓度降低,肺组织W/D比值降低,TLR4和NF-κB p65的表达下调,PA组T5时氧合指数升高(P＜0.05),其余指标差异无统计学意义(P＞0.05).结论预防性吸人全氟化碳2ml· kg-1·h-160 min可减轻兔ALI.
목적 평개전불화탄불동급약시궤대토급성폐손상(ALI)적영향.방법 신서란대백토18지,체중1.8～ 2.4 kg,50～ 60일령,자웅불구.채용수궤수자표법,장기분위3조(n=6):ALI조、전불화탄예방성급약조(PP조)화전불화탄치료성급약조(PA조).ALI조궤계통기60 min시,우5 min내경내정맥주사유산0.1 ml/kg제비ALI모형,분3차주사완필;PP조흡인전불화탄2ml· kg-1·h-160 min,연후제비ALI모형;PA조궤계통기60 min시제비ALI모형,모형제비성공후흡입전불화탄2 ml· kg-1·h-160 min.분별우마취은정30 min(T0)、궤계통기60 min혹전불화탄예방성급약후60 min(T1)、조모후30 min(T2)、조모후60 min(T3)、조모후90 min(T4)화조모후150 min(T5)시,취동맥혈양행혈기분석,계산양합지수.우T5시,수집지기관폐포관세액(BALF),연후처사대서,취폐조직,채용ELISA법검측BALF중TNF-α화IL-1β적농도,계산폐조직습중/간중비치(W/D비치),채용Western blot법검측Toll양수체4(TLR4)화NF-κB p65적표체수평.결과 여T0화T1시비교,3조T2-5시양합지수균강저(P＜0.05);여ALI조비교,PP조T2-5시양합지수승고,BALF중TNF-α화IL-1β적농도강저,폐조직W/D비치강저,TLR4화NF-κB p65적표체하조,PA조T5시양합지수승고(P＜0.05),기여지표차이무통계학의의(P＞0.05).결론 예방성흡인전불화탄2ml· kg-1·h-160 min가감경토ALI.
Objective To evaluate the effects of perfluorocarbon administered at different time points on acute lung injury (ALI) in rabbits.Methods Eighteen New Zealand white rabbits, weighing 1.8-2.4 kg, aged 50-60 days, were randomly divided into 3 groups (n=6 each) using a random number table: ALI group, preventive administration of perfluorocarbon group (group PP) , and therapeutic application of perfluorocarbon group (group PA).In group ALI, after the rabbits were mechanically ventilated for 60 min, ALI was induced with oleic acid 0.1 ml/kg injected via the internal jugular vein in three times within 5 min.In group PP, perfluorocarbon was inhaled at 2 ml · kg-1 · h-1 for 60 min, and then ALI was induced.In group PA, after the rabbits were mechanically ventilated for 60 min, ALI was induced, and then perfluorocarbon was inhaled at 2 ml · kg-1 · h 1 for 60 min.After 30 min stabilization (baseline, T0) , at 60 min of ventilation or at 60 min after preventive administration of perfluorocarbon (T1) , and at 30, 60, 90 and 150 min after ALI(T2-5), arterial blood samples were collected for blood gas analysis.Oxygenation index was calculated.Broncho-alveolar lavage fluid (BALF) was collected to determine the concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) (by ELISA) at T5.The animals were then sacrificed, and lungs were removed for determination of the expression of Toll-like receptor 4 (TLR4) and nuclear factor kappa B p65 (NF-κB p65) (by Western blot).Wet to dry lung weight ratio (W/D ratio) was calculated.Results Oxygenation index was significantly decreased at T2-5 than at T0 and T1 in the three groups.Compared to group ALI, oxygenation index was significantly increased at T2-5, the concentrations of TNF-α and IL-1β in BALF were decreased, W/D ratio was decreased, and the expression of TLR4 and NF-κB p65 was down-regulated in group PP, and oxygenation index was significantly increased at T5, and no significant change was found in the other parameters in group PA.Conclusion Preventive inhalation of perfluorocarbon 2 ml · kg-1 · h-1 can mitigate ALI in rabbits.