中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
11期
2754-2757
,共4页
朱明振%张婧%张迎娜%吕杰%赵雪%方华%崔新征%张清勇%高峰
硃明振%張婧%張迎娜%呂傑%趙雪%方華%崔新徵%張清勇%高峰
주명진%장청%장영나%려걸%조설%방화%최신정%장청용%고봉
分拣微管连接蛋白17%重症肌无力%乙酰胆碱受体%神经肌肉接头
分揀微管連接蛋白17%重癥肌無力%乙酰膽堿受體%神經肌肉接頭
분간미관련접단백17%중증기무력%을선담감수체%신경기육접두
Sorting nexin 17%Myasthenia gravis%Acetylcholine receptor%Neuromuscular junction
目的 比较分拣微管连接蛋白17(SNX17)在重症肌无力(MG)组和对照组肋间肌肌细胞中的表达,探讨其在乙酰胆碱受体(AChR)聚集中的作用.方法 取MG组和对照组肋间肌,分离出单根肌丝,用免疫荧光法观察肌丝AChR、SNX17分子形态及分布;实时荧光定量聚合酶链反应(FQ-PCR)、Western blot法比较两组肌细胞SNX17含量.结果 AChR位于肌纤维纵轴的中部,呈连续光滑的红色椭圆形突起.对照组AChR呈曲奇饼干样,内部有精细分支的网络状结构;MG组仅可观察到呈不连续完整的红色外周轮廓线,内部网状结构破碎,呈空泡样,但可观察到SNX17呈同样形状蓝色椭圆形突起,两者位置、形态一致.MG组SNX17 mRNA和蛋白含量(n=10,0.85±0.08;0.83 ±0.08)均明显高于对照组(n=14,0.57±0.09;0.65±0.06),差异有统计学意义(t=5.75 ,P ＜0.05;t=3.03 ,P ＜0.05).结论 MG患者肋间肌肌细胞SNX17表达增高,与AChR在神经肌肉接头处共定位,可能是促进AChR聚集,维护神经肌肉接头信号通路完整的重要分子.
目的 比較分揀微管連接蛋白17(SNX17)在重癥肌無力(MG)組和對照組肋間肌肌細胞中的錶達,探討其在乙酰膽堿受體(AChR)聚集中的作用.方法 取MG組和對照組肋間肌,分離齣單根肌絲,用免疫熒光法觀察肌絲AChR、SNX17分子形態及分佈;實時熒光定量聚閤酶鏈反應(FQ-PCR)、Western blot法比較兩組肌細胞SNX17含量.結果 AChR位于肌纖維縱軸的中部,呈連續光滑的紅色橢圓形突起.對照組AChR呈麯奇餅榦樣,內部有精細分支的網絡狀結構;MG組僅可觀察到呈不連續完整的紅色外週輪廓線,內部網狀結構破碎,呈空泡樣,但可觀察到SNX17呈同樣形狀藍色橢圓形突起,兩者位置、形態一緻.MG組SNX17 mRNA和蛋白含量(n=10,0.85±0.08;0.83 ±0.08)均明顯高于對照組(n=14,0.57±0.09;0.65±0.06),差異有統計學意義(t=5.75 ,P ＜0.05;t=3.03 ,P ＜0.05).結論 MG患者肋間肌肌細胞SNX17錶達增高,與AChR在神經肌肉接頭處共定位,可能是促進AChR聚集,維護神經肌肉接頭信號通路完整的重要分子.
목적 비교분간미관련접단백17(SNX17)재중증기무력(MG)조화대조조륵간기기세포중적표체,탐토기재을선담감수체(AChR)취집중적작용.방법 취MG조화대조조륵간기,분리출단근기사,용면역형광법관찰기사AChR、SNX17분자형태급분포;실시형광정량취합매련반응(FQ-PCR)、Western blot법비교량조기세포SNX17함량.결과 AChR위우기섬유종축적중부,정련속광활적홍색타원형돌기.대조조AChR정곡기병간양,내부유정세분지적망락상결구;MG조부가관찰도정불련속완정적홍색외주륜곽선,내부망상결구파쇄,정공포양,단가관찰도SNX17정동양형상람색타원형돌기,량자위치、형태일치.MG조SNX17 mRNA화단백함량(n=10,0.85±0.08;0.83 ±0.08)균명현고우대조조(n=14,0.57±0.09;0.65±0.06),차이유통계학의의(t=5.75 ,P ＜0.05;t=3.03 ,P ＜0.05).결론 MG환자륵간기기세포SNX17표체증고,여AChR재신경기육접두처공정위,가능시촉진AChR취집,유호신경기육접두신호통로완정적중요분자.
Objective By comparing sorting nexin 17 (SNX17) expression in intercostal muscle cells in myasthenia gravis (MG) group and control group, we study the role of SNX17 in the aggregation of acetylcholine receptors (AchRs).Methods Muscles were separated into a single cell.We used immunofluorescence to observe the form of acetylcholine receptor and SNX17 and compare the content of SNX17 in intercostal muscle cells by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and Western blotting between the two groups.Results The AChRs was located in the central part in the vertical axis of muscle fiber, a continuous smooth red oval bump.The AChRs outer looked like cookies with branch of internal fine reticular structure in control group.In MG group, only red outer contour, internal network structure, could be observed, with the cavitation, but that could be observed in the same blue oval shape.The location and shape were the same.The expression of SNX17 in MG group (n =10, 0.85 ± 0.08;0.83 ± 0.08) was significantly higher than in control group (n =14, 0.57 ± 0.09;0.65 ± 0.06) by Western blotting and FQ-PCR.The difference was statistically significant (t =5.75, P ＜ 0.05;t =3.03, P ＜0.05).Conclusion SNX17 in intercostal muscle cells is over-expressed in MG group, and positioned in the neuromuscular junction with AChRs.The SNX17 may play an important role in the neuromuscular junction structure and promote the aggregation of AChRs.
