CAJ | 학술논문

目的观察载紫杉醇(PTX)的脂质微泡(MBs)联合超声(US)辐照体外作用于人多发性骨髓瘤U266细胞的效应.方法采用机械振荡法制备PTX-MBs,镜下观察形态,检测其粒径、载药率、包封率和稳定性.检测单纯超声对细胞增殖和凋亡的影响.设Control组(Ⅰ)、PTX组(Ⅱ)、PTX+ US组(Ⅲ)、PTX-MBs组(Ⅳ)、PTX-MBs+ US组(Ⅴ),细胞计数试剂盒(CCK-8)法检测细胞的存活率,流式细胞术检测细胞的凋亡,软琼脂克隆实验检测细胞的克隆形成率.结果 PTX-MBs平均粒径为(353.9±25.7) nm,包封率为(86.25±7.31)％,载药率为(20.09±3.46)％.0.5 W/cm2的超声作用40 s对细胞增殖和凋亡无明显影响.各实验组中,Ⅴ组的细胞存活率[(32.89±5.59)％]较其他组[Ⅰ组:(98.42±1.47)％,Ⅱ组:(63.41±2.42)％,Ⅲ组:(58.79±4.16)％,Ⅳ组:(93.39±3.21)％]明显降低(P＜0.05),Ⅴ组的细胞凋亡率[(28.48±3.89)％]较其他组[Ⅰ组:(3.03±1.09)％,Ⅱ组:(16.69±3.14)％,Ⅲ组:(16.84±1.11)％,Ⅳ组:(4.43±1.58)％]明显增强(P＜0.05),Ⅴ组细胞的软琼脂克隆形成率[(6.00±1.00)％]较其他组[Ⅰ组:(35.00±5.00)％,Ⅱ组:(23.67±1.53)％,Ⅲ组:(20.00±2.65)％,Ⅳ组:(32.33±5.03)％]明显降低(P＜0.05).结论 PTX-MBs是良好的药物传递载体,联合超声辐照体外作用于多发性骨髓瘤细胞可显著抑制细胞增殖、诱导细胞凋亡以及抑制细胞克隆形成.
목적 관찰재자삼순(PTX)적지질미포(MBs)연합초성(US)복조체외작용우인다발성골수류U266세포적효응.방법 채용궤계진탕법제비PTX-MBs,경하관찰형태,검측기립경、재약솔、포봉솔화은정성.검측단순초성대세포증식화조망적영향.설Control조(Ⅰ)、PTX조(Ⅱ)、PTX+ US조(Ⅲ)、PTX-MBs조(Ⅳ)、PTX-MBs+ US조(Ⅴ),세포계수시제합(CCK-8)법검측세포적존활솔,류식세포술검측세포적조망,연경지극륭실험검측세포적극륭형성솔.결과 PTX-MBs평균립경위(353.9±25.7) nm,포봉솔위(86.25±7.31)％,재약솔위(20.09±3.46)％.0.5 W/cm2적초성작용40 s대세포증식화조망무명현영향.각실험조중,Ⅴ조적세포존활솔[(32.89±5.59)％]교기타조[Ⅰ조:(98.42±1.47)％,Ⅱ조:(63.41±2.42)％,Ⅲ조:(58.79±4.16)％,Ⅳ조:(93.39±3.21)％]명현강저(P＜0.05),Ⅴ조적세포조망솔[(28.48±3.89)％]교기타조[Ⅰ조:(3.03±1.09)％,Ⅱ조:(16.69±3.14)％,Ⅲ조:(16.84±1.11)％,Ⅳ조:(4.43±1.58)％]명현증강(P＜0.05),Ⅴ조세포적연경지극륭형성솔[(6.00±1.00)％]교기타조[Ⅰ조:(35.00±5.00)％,Ⅱ조:(23.67±1.53)％,Ⅲ조:(20.00±2.65)％,Ⅳ조:(32.33±5.03)％]명현강저(P＜0.05).결론 PTX-MBs시량호적약물전체재체,연합초성복조체외작용우다발성골수류세포가현저억제세포증식、유도세포조망이급억제세포극륭형성.
Objective To prepare paclitaxel-loaded lipid microbubbles (PTX-MBs) and investigate the effect of the MBs combined with ultrasound on multiple myeloma U266 cells in vitro.Methods PTX-MBs prepared by mechanic vibration method were observed under microscope.The size distribution, drug-loading efficiency and encapsulation efficiency were further detected.Ultrasound (US) was simply applied to multiple myeloma cells to observe the inhibitory effects.Cells were divided into Control group (Ⅰ), PTX group(Ⅱ), PTX + US group(Ⅲ), PTX-MBs group(Ⅳ), PTX-MBs + US group(Ⅴ).The inhibitory effect on cell proliferation was detected by cell counting kit-8 (CCK-8) method, the cell apoptosis was detected by flow cytometry (FCM), and the clone formation rate was detected by soft agar cloning method.Results PTX-MBs has good dispersion with the size [(353.9 ± 25.7) nm], encapsulation efficiency [(86.25 ± 7.31) ％], and drug-loading efficiency [(20.09 ± 3.46) ％].Ultrasound at 0.5 W/cm2 for40 s did no harm to cells.The survival rate was lower in PTX-MBs + US group[(32.89 ± 5.59) ％] than in other groups [Group Ⅰ : (98.42 ± 1.47) ％, Group Ⅱ : (63.41 ± 2.42) ％, Group Ⅲ : (58.79 ±4.16)％, Group Ⅳ: (93.39 ±3.21)％, P＜0.05].The apoptosis rate was higher in PTX-MBs + US group [(28.48 ± 3.89) ％] than in other groups [Group Ⅰ : (3.03 ± 1.09) ％, Group Ⅱ : (16.69 ± 3.14) ％, Group Ⅲ: (16.84 ± 1.11) ％, Group Ⅳ : (4.43 ± 1.58) ％, P ＜ 0.05].The clone formation rate was higher in PTX-MBs + US group [(6.00 ± 1.00) ％] than in other groups [Group Ⅰ : (35.00 ± 5.00) ％, Group Ⅱ : (23.67 ± 1.53) ％, Group Ⅲ : (20.00 ± 2.65) ％, Group Ⅳ : (32.33 ± 5.03) ％, P ＜ 0.05].Conclusion As drug delivery system, PTX-MBs can inhibit the proliferation, induce the apoptosis and inhibit the clone formation of multiple myeloma cells when combined with ultrasound.