中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
11期
2827-2829
,共3页
段仁全%李红军%谢京典%李小虎%彭操%陈剑英
段仁全%李紅軍%謝京典%李小虎%彭操%陳劍英
단인전%리홍군%사경전%리소호%팽조%진검영
胃癌%Rho相关结构域BTB蛋白质1%组织芯片%肿瘤抑制基因%RhoGTP酶
胃癌%Rho相關結構域BTB蛋白質1%組織芯片%腫瘤抑製基因%RhoGTP酶
위암%Rho상관결구역BTB단백질1%조직심편%종류억제기인%RhoGTP매
Gastric cancer%RhoBTB1%Tissue microarray%Tumor suppressor gene%RhoGTPase
目的 探讨胃癌组织芯片中Rho相关结构域BTB蛋白质1(RhoBTB1)的表达与临床病理特征的相关性及其临床意义.方法 采用组织芯片技术建立67例胃癌患者的组织芯片,应用免疫组织化学技术检测芯片中RhoBTB1的表达.结果 RhoBTB1在胃癌组织中表达的阳性率低于癌旁黏膜组织[(53±29)%比(63±28)%,Z=2.59,P<0.05];根据临床病理资料,胃癌组织中RhoBTB1的表达高低与病理分级明显相关,低/未分化腺癌RhoBTB1基因的阳性表达率低于中/高分化腺癌[(38±26)%比(65±25)%,Z=4.10,P<0.05],但其表达与性别、TNM分期、浸润深度、淋巴结转移、脉管转移及远处转移等因素无明显相关(P>0.05);RhoBTB1的表达与年龄、淋巴结转移数目和肿瘤大小等无明显相关(P>0.05).结论 RhoBTB1可能是与胃癌发生相关的候选抑癌基因,检测该基因的表达有助于评估胃癌的生物学行为和预后.
目的 探討胃癌組織芯片中Rho相關結構域BTB蛋白質1(RhoBTB1)的錶達與臨床病理特徵的相關性及其臨床意義.方法 採用組織芯片技術建立67例胃癌患者的組織芯片,應用免疫組織化學技術檢測芯片中RhoBTB1的錶達.結果 RhoBTB1在胃癌組織中錶達的暘性率低于癌徬黏膜組織[(53±29)%比(63±28)%,Z=2.59,P<0.05];根據臨床病理資料,胃癌組織中RhoBTB1的錶達高低與病理分級明顯相關,低/未分化腺癌RhoBTB1基因的暘性錶達率低于中/高分化腺癌[(38±26)%比(65±25)%,Z=4.10,P<0.05],但其錶達與性彆、TNM分期、浸潤深度、淋巴結轉移、脈管轉移及遠處轉移等因素無明顯相關(P>0.05);RhoBTB1的錶達與年齡、淋巴結轉移數目和腫瘤大小等無明顯相關(P>0.05).結論 RhoBTB1可能是與胃癌髮生相關的候選抑癌基因,檢測該基因的錶達有助于評估胃癌的生物學行為和預後.
목적 탐토위암조직심편중Rho상관결구역BTB단백질1(RhoBTB1)적표체여림상병리특정적상관성급기림상의의.방법 채용조직심편기술건립67례위암환자적조직심편,응용면역조직화학기술검측심편중RhoBTB1적표체.결과 RhoBTB1재위암조직중표체적양성솔저우암방점막조직[(53±29)%비(63±28)%,Z=2.59,P<0.05];근거림상병리자료,위암조직중RhoBTB1적표체고저여병리분급명현상관,저/미분화선암RhoBTB1기인적양성표체솔저우중/고분화선암[(38±26)%비(65±25)%,Z=4.10,P<0.05],단기표체여성별、TNM분기、침윤심도、림파결전이、맥관전이급원처전이등인소무명현상관(P>0.05);RhoBTB1적표체여년령、림파결전이수목화종류대소등무명현상관(P>0.05).결론 RhoBTB1가능시여위암발생상관적후선억암기인,검측해기인적표체유조우평고위암적생물학행위화예후.
Objective To investigate the relationship between the expression of RhoBTB1 and the clinicopathological factors in gastric cancer tissue microarray and its clinical significance.Methods Tissue microarray technology was adopted to establish a tissue chip of 67 cases of patients diagnosed with gastric cancer and immunohistochemistry technique was used to detect the expression of RhoBTB1 in this chip.Results The expression level of RhoBTB1 in the gastric cancer tissue is lower than that in the nomal mucosa tissue adjacent to gastric cancer [(53 ± 29) % vs.(63 ± 28) %, Z =2.59, P < 0.05].The expression level of RhoBTB1 in the gastric cancer tissue might have correlation with pathological grades, besides, higher the grade, lower the expression level of RhoBTB1 [(38 ±26)% vs.(65 ±25)%, Z =4.10, P < 0.05].However, the expression level of RhoBTB1 has no correlation with gender, TNM staging, depth of invasion, lymphatic metastasis, vascular metastasis and distant metastasis, neither with continuous variables, such as age and tumor size (P > 0.05).Conclusion RhoBTB1 might be one of candidate tumor suppressor genes that have correlation with the progression of gastric cancer.Detecting the expression level of RhoBTB1 might contribute to estimating the biological behaviors and prognosis of gastric cancer.