中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
11期
2830-2832
,共3页
陈辉%潘敦%谢文焌%李良庆
陳輝%潘敦%謝文焌%李良慶
진휘%반돈%사문준%리량경
胃癌%Rab23基因%音猬因子信号通路
胃癌%Rab23基因%音猬因子信號通路
위암%Rab23기인%음위인자신호통로
Gastric cancer%Rab23 gene%Sonic hedgehog signal pathway
目的 探讨Rab23基因在胃癌组织中的表达水平及其与临床病理、患者预后之间的关系.方法 实时荧光定量聚合酶链反应(FQ-PCR)方法和Western blot法检测60例新鲜胃癌组织及相应癌旁组织中Rab23 mRNA及蛋白的表达.免疫组织化学检测120例胃癌和正常胃黏膜石蜡组织中Rab23蛋白的表达水平.结果 胃癌组织中RAb23 mRNA(ACt:1.66 ±0.84)及蛋白(0.708 5 ±0.182 2)的表达水平较癌旁组织(ACt:0.59±0.34;0.297 4±0.1297)显著升高(t=9.07、14.24,P＜0.01).胃癌石蜡组织Rab23蛋白表达阳性率(41.7％,50/120)较正常胃黏膜石蜡组织(18.3％,11/60)显著升高(x2=9.72,P＜0.05).Rab23蛋白的表达与胃癌患者性别、肿瘤部位等无关(P＞0.05),而与年龄、肿瘤大小、分化程度、淋巴结转移、浸润深度、临床分期有关(P＜0.05).Rab23蛋白阴性、阳性和强阳性的5年生存率分别是48.6％、12.9％和5.3％(x2=35.49,P＜0.05).结论 Rab23基因的表达与胃癌的生物学行为密切相关.
目的 探討Rab23基因在胃癌組織中的錶達水平及其與臨床病理、患者預後之間的關繫.方法 實時熒光定量聚閤酶鏈反應(FQ-PCR)方法和Western blot法檢測60例新鮮胃癌組織及相應癌徬組織中Rab23 mRNA及蛋白的錶達.免疫組織化學檢測120例胃癌和正常胃黏膜石蠟組織中Rab23蛋白的錶達水平.結果 胃癌組織中RAb23 mRNA(ACt:1.66 ±0.84)及蛋白(0.708 5 ±0.182 2)的錶達水平較癌徬組織(ACt:0.59±0.34;0.297 4±0.1297)顯著升高(t=9.07、14.24,P＜0.01).胃癌石蠟組織Rab23蛋白錶達暘性率(41.7％,50/120)較正常胃黏膜石蠟組織(18.3％,11/60)顯著升高(x2=9.72,P＜0.05).Rab23蛋白的錶達與胃癌患者性彆、腫瘤部位等無關(P＞0.05),而與年齡、腫瘤大小、分化程度、淋巴結轉移、浸潤深度、臨床分期有關(P＜0.05).Rab23蛋白陰性、暘性和彊暘性的5年生存率分彆是48.6％、12.9％和5.3％(x2=35.49,P＜0.05).結論 Rab23基因的錶達與胃癌的生物學行為密切相關.
목적 탐토Rab23기인재위암조직중적표체수평급기여림상병리、환자예후지간적관계.방법 실시형광정량취합매련반응(FQ-PCR)방법화Western blot법검측60례신선위암조직급상응암방조직중Rab23 mRNA급단백적표체.면역조직화학검측120례위암화정상위점막석사조직중Rab23단백적표체수평.결과 위암조직중RAb23 mRNA(ACt:1.66 ±0.84)급단백(0.708 5 ±0.182 2)적표체수평교암방조직(ACt:0.59±0.34;0.297 4±0.1297)현저승고(t=9.07、14.24,P＜0.01).위암석사조직Rab23단백표체양성솔(41.7％,50/120)교정상위점막석사조직(18.3％,11/60)현저승고(x2=9.72,P＜0.05).Rab23단백적표체여위암환자성별、종류부위등무관(P＞0.05),이여년령、종류대소、분화정도、림파결전이、침윤심도、림상분기유관(P＜0.05).Rab23단백음성、양성화강양성적5년생존솔분별시48.6％、12.9％화5.3％(x2=35.49,P＜0.05).결론 Rab23기인적표체여위암적생물학행위밀절상관.
Objective To investigate the expression of Rab23 in gastric cancer and its association with the clinicopathological characteristics and prognosis of patients with gastric carcinoma.Methods The Rab23 mRNA and Rab23 protein expression levels in 60 fresh gastric carcinoma tissues and adjacent normal tissues were detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and Western blotting analysis.The Rab23 protein expression levels in 120 paraffin blocks of gastric cancer and 60 normal gastric tissues were determined by immunohistochemistry.Results The Rab23 mRNA (1.66 ±0.84) and Rab23 protein levels (0.708 5 ±0.182 2) in fresh gastric carcinoma tissues were significantly higher than those in adjacent normal tissues (0.59 ± 0.34, 0.297 4 ± 0.129 7, t =9.07, 14.24, P ＜0.01).Rab23 protein was positive in 41.7％ ％ of the paraffin blocks of gastric cancer and 18.3％ of normal gastric tissues.The difference was statistically significant (x2 =9.72, P ＜ 0.05).Rab23 protein expression was significantly correlated with age, tumor size, tumor differentiation, lymphatic metastasis, invasive depth, and clinical stage (P ＜ 0.05), but not with gender, and tumor areas (P ＞ 0.05).The five-year survival rate of Rab23-negative patients (48.6％) was significantly higher than that of Rab23-weakly positive (12.9％) and Rab23-strong positive patients (5.3 ％, x2 =35.49, P ＜ 0.05).Conclusion Rab23 expression is highly correlated with the biological behavior of gastric carcinoma.
