中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
Chinese Journal of Emergency Medicine
2015年
11期
1253-1256
,共4页
许磊%郭东风%刘国荣%施琴%赵昌明%杭敏
許磊%郭東風%劉國榮%施琴%趙昌明%杭敏
허뢰%곽동풍%류국영%시금%조창명%항민
脓毒症%血小板%造血系细胞特异蛋白-1%活化
膿毒癥%血小闆%造血繫細胞特異蛋白-1%活化
농독증%혈소판%조혈계세포특이단백-1%활화
Sepsis%Platelet%Hematopoietic lineage cell-specific protein-1 (HS1)%Activation
目的 探讨脓毒症时血小板功能的变化以及血小板中造血系细胞特异性蛋白-1(HS1)浓度和磷酸化HS1的变化和参与调节HS1磷酸化的因子.方法 收集并分离150例脓毒血症患者血小板和50例健康人富血小板血浆,使用微孔板吸附法和血小板凝集仪对两组人群血小板的黏附功能进行比较,同时使用ATP检测试剂盒检测两组人群洗涤血小板中ATP含量,对其释放功能进行比较;然后通过免疫印迹法对两组人群血小板总HS1 (t-HS1)和磷酸化HS1 (p-HS1)含量进行比较,随后使用LPS刺激分离健康人组血小板,并使用Src、Syk激酶特异性抑制剂验证该因子对HS1活化的调节作用.结果 数据显示脓毒症组血小板计数、血小板分布宽度(platelet distribution width,PDW)、平均血小板体积(mean platelet volumn,MPV)较健康人组差异具有统计学意义(P<0.01);脓毒症患者血小板黏附能力、聚集能力和释放能力均显著强于健康人;脓毒症血小板HS1以及p-HS1均显著高于健康人组,使用Src、Syk激酶抑制剂PP2和piceatannol可以显著的抑制LPS诱导的血小板HS1的磷酸化.结论 脓毒症中血小板HS1蛋白的与血小板功能密切相关,且有望成为脓毒症治疗的靶点.
目的 探討膿毒癥時血小闆功能的變化以及血小闆中造血繫細胞特異性蛋白-1(HS1)濃度和燐痠化HS1的變化和參與調節HS1燐痠化的因子.方法 收集併分離150例膿毒血癥患者血小闆和50例健康人富血小闆血漿,使用微孔闆吸附法和血小闆凝集儀對兩組人群血小闆的黏附功能進行比較,同時使用ATP檢測試劑盒檢測兩組人群洗滌血小闆中ATP含量,對其釋放功能進行比較;然後通過免疫印跡法對兩組人群血小闆總HS1 (t-HS1)和燐痠化HS1 (p-HS1)含量進行比較,隨後使用LPS刺激分離健康人組血小闆,併使用Src、Syk激酶特異性抑製劑驗證該因子對HS1活化的調節作用.結果 數據顯示膿毒癥組血小闆計數、血小闆分佈寬度(platelet distribution width,PDW)、平均血小闆體積(mean platelet volumn,MPV)較健康人組差異具有統計學意義(P<0.01);膿毒癥患者血小闆黏附能力、聚集能力和釋放能力均顯著彊于健康人;膿毒癥血小闆HS1以及p-HS1均顯著高于健康人組,使用Src、Syk激酶抑製劑PP2和piceatannol可以顯著的抑製LPS誘導的血小闆HS1的燐痠化.結論 膿毒癥中血小闆HS1蛋白的與血小闆功能密切相關,且有望成為膿毒癥治療的靶點.
목적 탐토농독증시혈소판공능적변화이급혈소판중조혈계세포특이성단백-1(HS1)농도화린산화HS1적변화화삼여조절HS1린산화적인자.방법 수집병분리150례농독혈증환자혈소판화50례건강인부혈소판혈장,사용미공판흡부법화혈소판응집의대량조인군혈소판적점부공능진행비교,동시사용ATP검측시제합검측량조인군세조혈소판중ATP함량,대기석방공능진행비교;연후통과면역인적법대량조인군혈소판총HS1 (t-HS1)화린산화HS1 (p-HS1)함량진행비교,수후사용LPS자격분리건강인조혈소판,병사용Src、Syk격매특이성억제제험증해인자대HS1활화적조절작용.결과 수거현시농독증조혈소판계수、혈소판분포관도(platelet distribution width,PDW)、평균혈소판체적(mean platelet volumn,MPV)교건강인조차이구유통계학의의(P<0.01);농독증환자혈소판점부능력、취집능력화석방능력균현저강우건강인;농독증혈소판HS1이급p-HS1균현저고우건강인조,사용Src、Syk격매억제제PP2화piceatannol가이현저적억제LPS유도적혈소판HS1적린산화.결론 농독증중혈소판HS1단백적여혈소판공능밀절상관,차유망성위농독증치료적파점.
Objective To explore the change of function and expression of hematopoietic lineage cell specific protein-1 (HS1) and phosphorylated HS1 (p-HIS) and factors devoting to HS1 phosphorylation in platelet with sepsis.Methods Plasma with rich platelet was collected from 150 sepsis patients and 50 healthy subjects, and comparison of platelets adhesion and aggregation were detected by micro-pore method and platelet aggregation instrument.Meanwhile the ATP concentrations of washed platelet of two groups were detected by the kit to compare release reaction.And then total HS1 (t-HIS) and p-HS1 of platelet from two groups were compared by using western blot.Afterwards the specific inhibitors of Src and Syk were used to verify the HS1 activation regulated by Src and Syk in LPS-induced cell model.Results The significant differences were present between healthy subjects and sepsis patients in platelet counts, platelet distribution width (PDW) and mean platelet volume (MPV) (P < 0.01).The data showed the sepsis patients had greater ability than healthy subjects in adhesion, aggregation and release reaction.Meanwhile the platelets of sepsis patients had higher concentration of t-HS1 and p-HS1 than healthy subjects, and the specific inhibitors of Src and Syk , PP2 and piceatannol, inhibited the increase in p-HS1 in LPS-induced cell model.Conclusions Function of platelet is closely related to HS1 in sepsis and it will be a target for sepsis therapy.