中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
11期
2803-2806
,共4页
傅超锋%庄源东%陈春美%王春华%赵伟%MD GOLAM KIBRIA
傅超鋒%莊源東%陳春美%王春華%趙偉%MD GOLAM KIBRIA
부초봉%장원동%진춘미%왕춘화%조위%MD GOLAM KIBRIA
脊髓缺血性损伤%炎症%修复%脱噬作用
脊髓缺血性損傷%炎癥%脩複%脫噬作用
척수결혈성손상%염증%수복%탈서작용
Spinal cord ischemia injury%Inflammation%Self-rehabilitation%Apoptosis
目的 观察SD大鼠腰动脉结扎脊髓缺血损伤后Fas、含半胱氨酰天冬氨酸特异性蛋白酶-3(Caspase-3)、肿瘤坏死因子-α(TNF-α)、核因子-κB(NF-κB)、神经生长因子(NGF)、生长相关蛋白-43(GAP-43)及脑源性神经生长因子(BDNF)表达的变化,探讨SD大鼠脊髓缺血性损伤后自我修复的过程.方法 56只SD大鼠随机分为缺血组和假手术组.假手术组14只,缺血组42只,缺血组分别再随机分为缺血1、2、4、6、8、12和24h组,以假手术组相同阶段的脊髓为对照,采用神经功能评定法(BMS评分)评估各组行为学的变化,并采用免疫组织化学方法检测脊髓中Fas、Caspase-3、TNF-α、NF-κB、NGF、GAP-43及BDNF的表达变化.结果 缺血组SD实验大鼠BMS评分在缺血后立即出现显著降低[(0.58 ±0.66)分],至缺血后24h逐渐恢复至正常水平[(8.33±0.61)分],与假手术组比较差异无统计学意义(P＞0.05),免疫组织化学染色结果显示Fas、Caspase-3、TNF-α、NF-κB表达的阳性细胞数在缺血后立即显著增多,其值分别为(45.50±4.18、47.50±3.27、53.50±7.71、57.83±4.79),与假手术组比较差异均有统计学意义(P＜0.05),除Fas外(7.50±1.87),其余各个指标至缺血后24h逐渐恢复至正常水平,与假手术组比较差异均无统计学意义(P＞0.05).GAP-43在缺血后阳性表达细胞数立即增多(6.33±1.21),缺血后各组与假手术组比较差异均有统计学意义(P＜0.05),NGF在缺血后8 h(37.33±1.37)阳性表达细胞数才开始增多,随后逐渐升高,缺血后8、12、24 h与假手术组比较均有统计学意义(P＜0.05),BDNF阳性表达细胞数在缺血后1、2h(22.83±1.94、23.83±1.94)显著低于假手术组,于缺血后4 h(31.33±2.16)开始上升,随后逐渐升高,缺血后4、6、8、12、24 h与假手术组比较差异均有统计学意义(P＜0.05).结论 SD大鼠全部腰动脉结扎致脊髓缺血性损伤后24 h可恢复至正常水平,Fas、Caspase-3、TNF-α、NF-κB表达降低及NGF、GAP-43、BDNF表达增强可能与其自我恢复机制有关.
目的 觀察SD大鼠腰動脈結扎脊髓缺血損傷後Fas、含半胱氨酰天鼕氨痠特異性蛋白酶-3(Caspase-3)、腫瘤壞死因子-α(TNF-α)、覈因子-κB(NF-κB)、神經生長因子(NGF)、生長相關蛋白-43(GAP-43)及腦源性神經生長因子(BDNF)錶達的變化,探討SD大鼠脊髓缺血性損傷後自我脩複的過程.方法 56隻SD大鼠隨機分為缺血組和假手術組.假手術組14隻,缺血組42隻,缺血組分彆再隨機分為缺血1、2、4、6、8、12和24h組,以假手術組相同階段的脊髓為對照,採用神經功能評定法(BMS評分)評估各組行為學的變化,併採用免疫組織化學方法檢測脊髓中Fas、Caspase-3、TNF-α、NF-κB、NGF、GAP-43及BDNF的錶達變化.結果 缺血組SD實驗大鼠BMS評分在缺血後立即齣現顯著降低[(0.58 ±0.66)分],至缺血後24h逐漸恢複至正常水平[(8.33±0.61)分],與假手術組比較差異無統計學意義(P＞0.05),免疫組織化學染色結果顯示Fas、Caspase-3、TNF-α、NF-κB錶達的暘性細胞數在缺血後立即顯著增多,其值分彆為(45.50±4.18、47.50±3.27、53.50±7.71、57.83±4.79),與假手術組比較差異均有統計學意義(P＜0.05),除Fas外(7.50±1.87),其餘各箇指標至缺血後24h逐漸恢複至正常水平,與假手術組比較差異均無統計學意義(P＞0.05).GAP-43在缺血後暘性錶達細胞數立即增多(6.33±1.21),缺血後各組與假手術組比較差異均有統計學意義(P＜0.05),NGF在缺血後8 h(37.33±1.37)暘性錶達細胞數纔開始增多,隨後逐漸升高,缺血後8、12、24 h與假手術組比較均有統計學意義(P＜0.05),BDNF暘性錶達細胞數在缺血後1、2h(22.83±1.94、23.83±1.94)顯著低于假手術組,于缺血後4 h(31.33±2.16)開始上升,隨後逐漸升高,缺血後4、6、8、12、24 h與假手術組比較差異均有統計學意義(P＜0.05).結論 SD大鼠全部腰動脈結扎緻脊髓缺血性損傷後24 h可恢複至正常水平,Fas、Caspase-3、TNF-α、NF-κB錶達降低及NGF、GAP-43、BDNF錶達增彊可能與其自我恢複機製有關.
목적 관찰SD대서요동맥결찰척수결혈손상후Fas、함반광안선천동안산특이성단백매-3(Caspase-3)、종류배사인자-α(TNF-α)、핵인자-κB(NF-κB)、신경생장인자(NGF)、생장상관단백-43(GAP-43)급뇌원성신경생장인자(BDNF)표체적변화,탐토SD대서척수결혈성손상후자아수복적과정.방법 56지SD대서수궤분위결혈조화가수술조.가수술조14지,결혈조42지,결혈조분별재수궤분위결혈1、2、4、6、8、12화24h조,이가수술조상동계단적척수위대조,채용신경공능평정법(BMS평분)평고각조행위학적변화,병채용면역조직화학방법검측척수중Fas、Caspase-3、TNF-α、NF-κB、NGF、GAP-43급BDNF적표체변화.결과 결혈조SD실험대서BMS평분재결혈후립즉출현현저강저[(0.58 ±0.66)분],지결혈후24h축점회복지정상수평[(8.33±0.61)분],여가수술조비교차이무통계학의의(P＞0.05),면역조직화학염색결과현시Fas、Caspase-3、TNF-α、NF-κB표체적양성세포수재결혈후립즉현저증다,기치분별위(45.50±4.18、47.50±3.27、53.50±7.71、57.83±4.79),여가수술조비교차이균유통계학의의(P＜0.05),제Fas외(7.50±1.87),기여각개지표지결혈후24h축점회복지정상수평,여가수술조비교차이균무통계학의의(P＞0.05).GAP-43재결혈후양성표체세포수립즉증다(6.33±1.21),결혈후각조여가수술조비교차이균유통계학의의(P＜0.05),NGF재결혈후8 h(37.33±1.37)양성표체세포수재개시증다,수후축점승고,결혈후8、12、24 h여가수술조비교균유통계학의의(P＜0.05),BDNF양성표체세포수재결혈후1、2h(22.83±1.94、23.83±1.94)현저저우가수술조,우결혈후4 h(31.33±2.16)개시상승,수후축점승고,결혈후4、6、8、12、24 h여가수술조비교차이균유통계학의의(P＜0.05).결론 SD대서전부요동맥결찰치척수결혈성손상후24 h가회복지정상수평,Fas、Caspase-3、TNF-α、NF-κB표체강저급NGF、GAP-43、BDNF표체증강가능여기자아회복궤제유관.
Objective To investigate the change of cysteinyl aspartate-specific protease-3 (Caspase-3), tumor necrosis factor-α (TNF-α), nuclear factor-κB (NF-κB), nerve growth factor (NGF), growth associated protein 43 (GAP-43), and brain derived neurotrophic factor (BDNF) expression after spinal cord ischemia injury in the SD rats by ligating lumbar arteries, and observe the process of self-repair after spinal cord ischemia injury in the SD rats.Methods Fifty-six SD rats were divide into ischemia group (n =42) and sham operation group (n =12) randomly.The SD rats in ischemia group were subdivided into 1,2, 4, 6, 8, 12 and 24 h subgroups.BMS score was adopted to assess the change of behavior.The expression of Fas, Caspase-3, TNF-α, NF-κB, NGF, GAP-43 and BDNF was detected by immunohistochemistry.Results In ischemia group, BMS score was obvious decrease immediately after ischemia injury (0.58 ±0.66) and return to normal level (8.33 ±0.61) 24 h after ischemia injury, there was no significant difference compared with control group (P ＞ 0.05).Immunohistochemical study showed obvious increase were found in Fas, Caspase-3, TNF-α, NF-κB activity in ischemia group, the value were (45.50 ±4.18, 47.50 ±3.27, 53.50 ±7.71,57.83 ±4.79) respectively,which had statistically difference compared with control group (P ＜ 0.05), and return to normal level 24 h after ischemia injury except Fas (7.50 ± 1.87).GAP-43 positive cell number increased immediately after ischemia injury (6.33 ± 1.21) and had statistically difference compared with control group in each group (P ＜0.05).The number of NGF positive cell were increase 8 h after ischemia injury (37.33 ± 1.37) and in 8, 12, 24 h after ischemia injury there had statistically difference compared with control group.BDNF activity cell number were significantly lower than control group 1 h and 2 h after ischemia injury (22.83 ± 1.94, 23.83 ± 1.94) and increased 4 h after ischemia injury (31.33 ±2.16), compared with control group there were significant difference (P ＜ 0.05) at 4, 6, 8, 12, 24 h after ischemia injury.Conclusion Ischemia injury in spinal cord would repair 24 h after ischemia in SD rats with ligation of all lumbar arteries.The mechanism of self-repair may be related to the down-regulation of Fas, Caspase-3, TNF-α, NF-κB expression and the up-regulation of NGF, GAP-43, and BDNF expression.
