CAJ | 학술논문

以酱香大曲为研究材料,从中初筛选出14株细菌.利用透明圈初筛从中筛选出4株产淀粉酶的菌株以及2株产蛋白酶的菌株,采用液体发酵培养方法,考察其中的淀粉酶活力以及蛋白酶活力等指标,从而筛选出1株酶活力较高的菌株,命名为3J-1,其淀粉酶活力为3.72 U/mL,蛋白酶活力为70.15 U/mL.菌株3J-1通过16S rD-NA分子生物学鉴定为枯草芽孢杆菌属(Bacillus subtilis).
이장향대곡위연구재료,종중초사선출14주세균.이용투명권초사종중사선출4주산정분매적균주이급2주산단백매적균주,채용액체발효배양방법,고찰기중적정분매활력이급단백매활력등지표,종이사선출1주매활력교고적균주,명명위3J-1,기정분매활력위3.72 U/mL,단백매활력위70.15 U/mL.균주3J-1통과16S rD-NA분자생물학감정위고초아포간균속(Bacillus subtilis).
Fourteen bacteria strains were isolated from Jiangxiang Daqu. 4 amylase-producing strains and 2 protease-producing strains were screened out by transparent circle method. Then strain 3J-1 (its amylase activity as 3.72 U/mL, its protease activity as 70.15 U/mL) was further screened out by measuring the activities of amylase and protease through liquid fermentation culture of those strains. Strain 3J-1 was then iden-tified as Bacillus subtilis through 16S rDNA sequencing&molecular biology.