中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
Chinese Journal of Physical Medicine and Rehabilitation
2015年
10期
733-738
,共6页
刘芳%黄海龙%陈红%侯丽敏%邓雨果%尤春景
劉芳%黃海龍%陳紅%侯麗敏%鄧雨果%尤春景
류방%황해룡%진홍%후려민%산우과%우춘경
高压氧%局灶性脑缺血%神经干细胞%增殖%神经元
高壓氧%跼竈性腦缺血%神經榦細胞%增殖%神經元
고압양%국조성뇌결혈%신경간세포%증식%신경원
Hyperbaric oxygen%Focal cerebral ischemia%Neural stem cells%Proliferation%Neuron
目的 观察高压氧(HBO)干预对成年大鼠急性局灶性脑缺血后海马齿状回颗粒下区(SGZ)神经干细胞增殖及向神经元分化的影响.方法 选取48只成年雄性SD大鼠,按随机数字表法分为模型组、高压氧组、高压空气组和常压氧组,每组12只.各组大鼠均进行线栓法MCAO制模,除模型组制模后不接受任何干预外,其余3组均于线栓插入后2h分别给予高压氧,高压空气和常压氧干预,每日1次.采用免疫荧光双重标记制模成功后第2、3、7和14天脑梗死侧SGZ区增殖的神经干细胞(BrdU+/nestin+)及其分化的神经元(BrdU +/DCX+),并在荧光显微镜下计数.结果 制模成功后第2天,高压氧组SGZ区BrdU/nestin和BrdU/DCX的共标细胞数分别为(2340.45±1109.59)个和(5520.66±1103.09)个,分别与常压氧组和模型组同时间点相同细胞比较,差异均有统计学意义(P＜0.05);制模成功后第3和第7天,高压氧组的BrdU/nestin和BrdU/DCX共标细胞数均显著高于高压空气组、常压氧组和模型组,差异均有统计学意义(P＜0.05),且制模成功后第14天,高压氧组的BrdU/DCX共标细胞数亦显著高于高压空气组、常压氧组和模型组,差异均有统计学意义(P＜0.05).结论 高压氧干预可显著促进成年大鼠梗死侧SGZ区神经干细胞的增殖和向神经元分化.
目的 觀察高壓氧(HBO)榦預對成年大鼠急性跼竈性腦缺血後海馬齒狀迴顆粒下區(SGZ)神經榦細胞增殖及嚮神經元分化的影響.方法 選取48隻成年雄性SD大鼠,按隨機數字錶法分為模型組、高壓氧組、高壓空氣組和常壓氧組,每組12隻.各組大鼠均進行線栓法MCAO製模,除模型組製模後不接受任何榦預外,其餘3組均于線栓插入後2h分彆給予高壓氧,高壓空氣和常壓氧榦預,每日1次.採用免疫熒光雙重標記製模成功後第2、3、7和14天腦梗死側SGZ區增殖的神經榦細胞(BrdU+/nestin+)及其分化的神經元(BrdU +/DCX+),併在熒光顯微鏡下計數.結果 製模成功後第2天,高壓氧組SGZ區BrdU/nestin和BrdU/DCX的共標細胞數分彆為(2340.45±1109.59)箇和(5520.66±1103.09)箇,分彆與常壓氧組和模型組同時間點相同細胞比較,差異均有統計學意義(P＜0.05);製模成功後第3和第7天,高壓氧組的BrdU/nestin和BrdU/DCX共標細胞數均顯著高于高壓空氣組、常壓氧組和模型組,差異均有統計學意義(P＜0.05),且製模成功後第14天,高壓氧組的BrdU/DCX共標細胞數亦顯著高于高壓空氣組、常壓氧組和模型組,差異均有統計學意義(P＜0.05).結論 高壓氧榦預可顯著促進成年大鼠梗死側SGZ區神經榦細胞的增殖和嚮神經元分化.
목적 관찰고압양(HBO)간예대성년대서급성국조성뇌결혈후해마치상회과립하구(SGZ)신경간세포증식급향신경원분화적영향.방법 선취48지성년웅성SD대서,안수궤수자표법분위모형조、고압양조、고압공기조화상압양조,매조12지.각조대서균진행선전법MCAO제모,제모형조제모후불접수임하간예외,기여3조균우선전삽입후2h분별급여고압양,고압공기화상압양간예,매일1차.채용면역형광쌍중표기제모성공후제2、3、7화14천뇌경사측SGZ구증식적신경간세포(BrdU+/nestin+)급기분화적신경원(BrdU +/DCX+),병재형광현미경하계수.결과 제모성공후제2천,고압양조SGZ구BrdU/nestin화BrdU/DCX적공표세포수분별위(2340.45±1109.59)개화(5520.66±1103.09)개,분별여상압양조화모형조동시간점상동세포비교,차이균유통계학의의(P＜0.05);제모성공후제3화제7천,고압양조적BrdU/nestin화BrdU/DCX공표세포수균현저고우고압공기조、상압양조화모형조,차이균유통계학의의(P＜0.05),차제모성공후제14천,고압양조적BrdU/DCX공표세포수역현저고우고압공기조、상압양조화모형조,차이균유통계학의의(P＜0.05).결론 고압양간예가현저촉진성년대서경사측SGZ구신경간세포적증식화향신경원분화.
Objective To investigate the effect of hyperbaric oxygen intervention at different time on the neural stem cell proliferation and differentiation in dentate gyrus subgranular zone (SGZ) of adult rats after acute focal cerebral ischemia.Methods Totally 48 Sprague-Dawley male adult rats were randomly divided into a middle cerebral artery occlusion (MCAO) group, a hyperbaric oxygen group, a hyperbaric air group and a normobaric oxygen group, each of 12.A middle cerebral artery occlusion (MCAO) model was induced to all rats using the modified Zea-Longa's method of intraluminal filament occlusion, Except the MCAO group, the other 3 groups received corresponding hyperbaric oxygen, hyperbaric air and normobaric oxygen intervention once a day two hours after the suture insertion.The rats were sacrificed for double-label immunofluorescent analysis at 2 days, 3 days, 7 days and 14 days after brain ischemia.BrdU +/nestin + labeled the proliferated neural stem cells, and BrdU +/DCX + labeled its differentiated derivates, early neurons, in SGZ of ischemic hippocampus dentate gyrus.Also, the cell number was calculated under the fluorescence microscope.Results Two days after brain ischemia, the numbers of BrdU/nestin and BrdU/DCX cells in SGZ in the hyperbaric oxygen group were (2340.45 ± 1109.59) and (5520.66 ± 1103.09) respectively, which had increased significantly, compared with the hyperbaric air group and normobaric oxygen group (P ＜ 0.05).Three and 7 days after brain ischemia, the numbers of BrdU/nestin and BrdU/DCX cells in SGZ in the hyperbaric oxygen group had shown significant increase compared with the other 3 groups (P ＜ 0.05).Fourteen days after brain ischemia, the numbers of BrdU/DCX cells in SGZ in the hyperbaric oxygen group had significantly increased compared with the hyperbaric air group, normobaric oxygen group and the MCAO group (P ＜ 0.05).Conclusion Hyperbaric oxygen promotes the proliferation and differentiation of neural stem cells in ischemic SGZ.
