中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
11期
2636-2638
,共3页
王百林%苑伟%翟淑萍%程海坤%王蕊%赵曼
王百林%苑偉%翟淑萍%程海坤%王蕊%趙曼
왕백림%원위%적숙평%정해곤%왕예%조만
梗阻性黄疸%利胆消黄汤%肝细胞%线粒体
梗阻性黃疸%利膽消黃湯%肝細胞%線粒體
경조성황달%리담소황탕%간세포%선립체
Obstructive jaundice%Gallbladder eliminate yellow soup%Liver cells%Mitochondrial
目的 观察利胆消黄汤对梗阻性黄疸兔肝细胞线粒体DNA损伤的修复作用.方法 新西兰白兔30只,随机分为对照组、梗阻性黄疸组和中药组,采用胆总管中段捆扎法制备兔梗阻性黄疸模型;中药组于造模后第7天开始给予利胆消黄汤灌胃,每次50 ml,每天1次.于造模后第14天取各组兔肝组织,利用实时荧光定量聚合酶链反应(FQ-PCR)方法对各组兔肝细胞线粒体DNA(mtDNA)拷贝数及编码基因线粒体RNA (mtRNA)含量的检测.结果 造模后第14天,梗阻性黄疸组兔肝组织mtDNA拷贝数和编码基因mtRNAs(ND1)的含量(1.35±0.34、4.89 ±0.72)均较对照组(4.25 ±0.61、10.11 ±0.52)降低,差异有统计学意义(P＜0.05);中药组肝组织mtDNA拷贝数和编码基因mtRNAs(ND1)的含量(4.01 ±0.78、8.66 ±0.94)与梗阻性黄疸组比较均升高,差异有统计学意义(P＜0.05).结论 利胆消黄汤对兔肝细胞线粒体内氧化应激所导致的损伤具有保护和修复作用,其机制是对受损的肝细胞线粒体DNA具有修复作用.
目的 觀察利膽消黃湯對梗阻性黃疸兔肝細胞線粒體DNA損傷的脩複作用.方法 新西蘭白兔30隻,隨機分為對照組、梗阻性黃疸組和中藥組,採用膽總管中段捆扎法製備兔梗阻性黃疸模型;中藥組于造模後第7天開始給予利膽消黃湯灌胃,每次50 ml,每天1次.于造模後第14天取各組兔肝組織,利用實時熒光定量聚閤酶鏈反應(FQ-PCR)方法對各組兔肝細胞線粒體DNA(mtDNA)拷貝數及編碼基因線粒體RNA (mtRNA)含量的檢測.結果 造模後第14天,梗阻性黃疸組兔肝組織mtDNA拷貝數和編碼基因mtRNAs(ND1)的含量(1.35±0.34、4.89 ±0.72)均較對照組(4.25 ±0.61、10.11 ±0.52)降低,差異有統計學意義(P＜0.05);中藥組肝組織mtDNA拷貝數和編碼基因mtRNAs(ND1)的含量(4.01 ±0.78、8.66 ±0.94)與梗阻性黃疸組比較均升高,差異有統計學意義(P＜0.05).結論 利膽消黃湯對兔肝細胞線粒體內氧化應激所導緻的損傷具有保護和脩複作用,其機製是對受損的肝細胞線粒體DNA具有脩複作用.
목적 관찰리담소황탕대경조성황달토간세포선립체DNA손상적수복작용.방법 신서란백토30지,수궤분위대조조、경조성황달조화중약조,채용담총관중단곤찰법제비토경조성황달모형;중약조우조모후제7천개시급여리담소황탕관위,매차50 ml,매천1차.우조모후제14천취각조토간조직,이용실시형광정량취합매련반응(FQ-PCR)방법대각조토간세포선립체DNA(mtDNA)고패수급편마기인선립체RNA (mtRNA)함량적검측.결과 조모후제14천,경조성황달조토간조직mtDNA고패수화편마기인mtRNAs(ND1)적함량(1.35±0.34、4.89 ±0.72)균교대조조(4.25 ±0.61、10.11 ±0.52)강저,차이유통계학의의(P＜0.05);중약조간조직mtDNA고패수화편마기인mtRNAs(ND1)적함량(4.01 ±0.78、8.66 ±0.94)여경조성황달조비교균승고,차이유통계학의의(P＜0.05).결론 리담소황탕대토간세포선립체내양화응격소도치적손상구유보호화수복작용,기궤제시대수손적간세포선립체DNA구유수복작용.
Objective To observe the protective effect of Lidanxiaohuang decoction on hepatocyte mitochondrial DNA (mtDNA) damage in rabbits with obstructive jaundice.Methods The 30 New Zealand rabbits were divided into the control group, the obstructive jaundice group and the Chinese medicines group randomly.The rabbit obstructive jaundice models were established by ligating the middle of the biliary duct.The Chinese medicines group rabbits were treated with 50 ml Lidanxiaohuang docoction once every day by gavages for 7 days after modeling.At 14th day after modeling, the liver tissues were taken out for detection of mtDNA and its coding gene mtRNAs in hepatocytes by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).Results At 14th day after modeling, the copy number of mtDNA and the amount of the gene encoding mtRNAs (ND1) in rabbit liver tissues in the obstructive jaundice group were 1.35 ±0.34 and 4.89 ±0.72, which were significantly lower than those (4.25 ±0.61, and 10.11 ± 0.52 correspondingly) in the control group (P ＜ 0.05).The copy number of mtDNA and the amount of the gene encoding mtRNAs (ND1) in rabbit liver tissues in the Chinese medicines group were 4.01 ±0.78 and 8.66 ± 0.94, which were significantly higher than those in the obstructive jaundice group (P ＜ 0.05).Conclusion The Lidanxiaohuang decoction could protect and repair the oxidative damage of hepatocyte mitochondria in rabbits with obstructive jaundice, which may be related to repair of hepatocyte mtDNA damage.
