黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
Heilongjiang Agricultural Sciences
2015年
10期
141-145
,共5页
黄艳宁%谢进%范海珊%彭斯文%徐瑞%朱校奇
黃豔寧%謝進%範海珊%彭斯文%徐瑞%硃校奇
황염저%사진%범해산%팽사문%서서%주교기
铁皮石斛%组培苗%移栽基质%理化特性
鐵皮石斛%組培苗%移栽基質%理化特性
철피석곡%조배묘%이재기질%이화특성
DendrobiumofficinaleKimuraetMigo%invitro%culturesubstrate%physicochemicalcharacteristic
基质理化性质是影响组培苗移栽时生根成活的关键因素 ,通过不同基质种类的不同比例配比 ,对铁皮石斛培养基质的理化性质测定和调查组培苗成活率等指标进行研究.结果表明 :铁皮石斛组培苗在各配方的生根基质中较适宜的理化性质配方为锯木:泥炭:珍珠岩=1:2:1 ,其容重0 .11 g·cm-3 ,总孔隙度60 .58% ,毛管孔隙度14 .25% ,非毛管孔隙度71 .56% ,大小孔隙比1:1 .3.基质中的氮磷钾养分含量分别为0 .059、0 .016、0 .153 g·kg-1 ,pH6 .8 ,电导率为0 .90 Ec.铁皮石斛在不同基质栽培中的成活率和生根、生长有显著影响 ,栽培于锯木:泥炭:珍珠岩=1:2:1基质中的组培苗的成活率、生根情况和生长情况均显著高于其它基质配方 ,是一种比较适合铁皮石斛组培苗生长的栽培基质.
基質理化性質是影響組培苗移栽時生根成活的關鍵因素 ,通過不同基質種類的不同比例配比 ,對鐵皮石斛培養基質的理化性質測定和調查組培苗成活率等指標進行研究.結果錶明 :鐵皮石斛組培苗在各配方的生根基質中較適宜的理化性質配方為鋸木:泥炭:珍珠巖=1:2:1 ,其容重0 .11 g·cm-3 ,總孔隙度60 .58% ,毛管孔隙度14 .25% ,非毛管孔隙度71 .56% ,大小孔隙比1:1 .3.基質中的氮燐鉀養分含量分彆為0 .059、0 .016、0 .153 g·kg-1 ,pH6 .8 ,電導率為0 .90 Ec.鐵皮石斛在不同基質栽培中的成活率和生根、生長有顯著影響 ,栽培于鋸木:泥炭:珍珠巖=1:2:1基質中的組培苗的成活率、生根情況和生長情況均顯著高于其它基質配方 ,是一種比較適閤鐵皮石斛組培苗生長的栽培基質.
기질이화성질시영향조배묘이재시생근성활적관건인소 ,통과불동기질충류적불동비례배비 ,대철피석곡배양기질적이화성질측정화조사조배묘성활솔등지표진행연구.결과표명 :철피석곡조배묘재각배방적생근기질중교괄의적이화성질배방위거목:니탄:진주암=1:2:1 ,기용중0 .11 g·cm-3 ,총공극도60 .58% ,모관공극도14 .25% ,비모관공극도71 .56% ,대소공극비1:1 .3.기질중적담린갑양분함량분별위0 .059、0 .016、0 .153 g·kg-1 ,pH6 .8 ,전도솔위0 .90 Ec.철피석곡재불동기질재배중적성활솔화생근、생장유현저영향 ,재배우거목:니탄:진주암=1:2:1기질중적조배묘적성활솔、생근정황화생장정황균현저고우기타기질배방 ,시일충비교괄합철피석곡조배묘생장적재배기질.
The physical and chemical properties of matrix are the key factors for the somaclone took root trans-planting survival .Through the ratio of different proportions of various matrix ,determination of matriz physical and chemical properties of Dendrobium of f icinale Kimura et Migo and survey the somaclone indices such as survival rate were studied .The results showed that the Dendrobium of f icinale Kimura et Migo somaclone took root in the formula of the matrix was suitable in the physical and chemical properties of the formula for lum-ber :peat :pearlite was 1 :2:1 ,its bulk density was 0 .11 g·cm-3 ,60 .58% of the total porosity and capillary po-rosity were 14 .25% and 71 .56% ,the capillary porosity size void ratio was 1 :1 .3 .Matrix of NPK nutrient con-tent were 0 .059 ,0 .016 and 0 .153 g·kg-1 ,pH value was 6 .8 ,the conductivity was 0 .90 Ec .The survival rate , rooting and growth of Dendrobium of ficinale Kimura et Migo somaclone grew in sawing wood ,peat ,perlite of 1:2:1 matrix which were significantly higher than other matrix formula .It was more suitable for the Dendrobi-um of f icinale Kimura et Migo somaclone growth of cultivation matrix .