山地农业生物学报
山地農業生物學報
산지농업생물학보
Journal of Mountain Agriculture and Biology
2015年
4期
51-54
,共4页
张雪%周英%管静%鄢守群%俸婷婷
張雪%週英%管靜%鄢守群%俸婷婷
장설%주영%관정%언수군%봉정정
中药材%黄嘌呤氧化酶%抑制作用
中藥材%黃嘌呤氧化酶%抑製作用
중약재%황표령양화매%억제작용
Chinese herbal medicine%xanthine oxidase%inhibition
为研究老鹳草、大血藤、吴茱萸、锁阳及木瓜5种中药材提取物对黄嘌呤氧化酶的抑制作用.以别嘌呤醇作为阳性对照,采用紫外分光光度法在体外直接测定5种中药材不同浓度的提取物对黄嘌呤氧化酶的抑制作用.结果显示:5种中药材提取物(20 mg/mL)对黄嘌呤氧化酶的抑制率均大于50%,老鹳草的醇提物和水提物对黄嘌呤氧化酶的抑制作用最明显,其IC50分别为3.141 mg/mL、2.407 mg/mL.除老鹳草外,其余4种中药材的醇提物的IC50均小于水提物的IC50.表明5种中药材的醇提物和水提物对黄嘌呤氧化酶均具有较好的抑制作用,老鹳草的提取物抑制作用最明显.
為研究老鸛草、大血籐、吳茱萸、鎖暘及木瓜5種中藥材提取物對黃嘌呤氧化酶的抑製作用.以彆嘌呤醇作為暘性對照,採用紫外分光光度法在體外直接測定5種中藥材不同濃度的提取物對黃嘌呤氧化酶的抑製作用.結果顯示:5種中藥材提取物(20 mg/mL)對黃嘌呤氧化酶的抑製率均大于50%,老鸛草的醇提物和水提物對黃嘌呤氧化酶的抑製作用最明顯,其IC50分彆為3.141 mg/mL、2.407 mg/mL.除老鸛草外,其餘4種中藥材的醇提物的IC50均小于水提物的IC50.錶明5種中藥材的醇提物和水提物對黃嘌呤氧化酶均具有較好的抑製作用,老鸛草的提取物抑製作用最明顯.
위연구로관초、대혈등、오수유、쇄양급목과5충중약재제취물대황표령양화매적억제작용.이별표령순작위양성대조,채용자외분광광도법재체외직접측정5충중약재불동농도적제취물대황표령양화매적억제작용.결과현시:5충중약재제취물(20 mg/mL)대황표령양화매적억제솔균대우50%,로관초적순제물화수제물대황표령양화매적억제작용최명현,기IC50분별위3.141 mg/mL、2.407 mg/mL.제로관초외,기여4충중약재적순제물적IC50균소우수제물적IC50.표명5충중약재적순제물화수제물대황표령양화매균구유교호적억제작용,로관초적제취물억제작용최명현.
The aim of the project is to study the inhibitory effects on xanthine oxidase by the extracts from five Chinese herbal medicines:Geranium wilfordii Maxim, Sargentodoxa cuneata (Oliv.) Rehd. et Wils, Evodia rutaecarpa (Juss.) Benth, Cynomorium songaricum Rupr and Chaenomeles sinensis (Thouin) Koehne. We directly measure the inhibitory effects on xanthine oxidase by the extracts of different concentrations from five Chinese herbal medicines using UV spectrophotometry in vitro, with allopurinol as positive control. The inhi-bition rate on the xanthine oxidase by the extracts (20 mg/mL) from all five Chinese herbal medicines is greater than 50%. The inhibition on xanthine oxidase by the ethanol extracts and water extracts of Geranium wilfordii is the most obvious and the IC50 are 3. 141 mg/mL and 2. 407 mg/mL, respectively. Apart from Ge-ranium wilfordii, the IC50 of the ethanol extracts of remaining four Chinese herbal medicines is less than the IC50 of the water extracts. It is concluded that all the ethanol extracts and water extracts of the five Chinese herbal medicines have good inhibition on xanthine oxidase, with the inhibition by Geranium wilfordii extracts the most obvious.