CAJ | 학술논문

首次在长牡蛎(Crassostrea gigas)中克隆得到一种新的贝壳基质蛋白nacrein-like protein F3的全长cDNA序列。nacrein-like protein F3基因cDNA全长1499bp,其中编码区长度为1242bp,编码一条含413个氨基酸残基的多肽链。氨基酸序列比对和结构域分析均表明其为合浦珠母贝(Pinctada fu-cata)nacrein的同源蛋白,含有1个保守的α-碳酸酐酶结构域,但由于重复结构域的插入,α-碳酸酐酶结构域被间隔成2个亚结构域。系统进化分析显示nacrein-like protein F3与贻贝(Mytilus californianus) nacrein-like protein进化关系最近。此外,在软体动物中,双壳纲nacrein-like proteins进化速度相对较快,推测与寒武纪时期剧烈的环境变化有关,如影响贝壳形成的海水化学变化。
수차재장모려(Crassostrea gigas)중극륭득도일충신적패각기질단백nacrein-like protein F3적전장cDNA서렬。nacrein-like protein F3기인cDNA전장1499bp,기중편마구장도위1242bp,편마일조함413개안기산잔기적다태련。안기산서렬비대화결구역분석균표명기위합포주모패(Pinctada fu-cata)nacrein적동원단백,함유1개보수적α-탄산항매결구역,단유우중복결구역적삽입,α-탄산항매결구역피간격성2개아결구역。계통진화분석현시nacrein-like protein F3여이패(Mytilus californianus) nacrein-like protein진화관계최근。차외,재연체동물중,쌍각강nacrein-like proteins진화속도상대교쾌,추측여한무기시기극렬적배경변화유관,여영향패각형성적해수화학변화。
The full length cDNA sequence of nacrein-like protein F3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Nacrein-like protein F3 cDNA has 1499 bp, containing a 1242 bp CDS that encod a peptide of 413 amino acids. Multiple alignment and conserved domain analysis showed that this peptide is a homolog of nacrein (Pinctada fucata), containing a carbonic anhydrase (CA) domain, which is flanked by inserted repeat se-quences. Phylogenetic analysis indicated that nacrein-like protein F3 is more closely related to nacrein-like protein inMytilus californianus than other nacrein-like proteins. Moreover, the nacrein-like proteins in bivalves seem to evolve relatively fast, which is presumably caused by the fast changing Cambrian environment, especially the ma-rine chemical composition, which can affect shell biomineralization.