CAJ | 학술논문

针对目前海参养殖中水产微生态制剂品种单一、施用效果不稳定等现状,作者从地瓜参(Acaudina molpadioidea)肠道分离土著肠杆菌(Enterobacter),筛选到1株具有较好拮抗作用的海洋细菌C-5,该菌对副溶血弧菌(Vibrio parahaemolyticus)、溶藻弧菌(Vibrio alginolyticus)、杀鲑气单胞菌(Aeromonas salmonicida)等7种细菌有拮抗作用；通过形态观察、生理生化和16S rDNA基因序列分析鉴定为霍肠杆菌(Enterobacter hor-maechei)；通过卤虫(Artemia)和药敏实验检测该菌具有安全性,其对卤虫的72 h半致死浓度(LC50)为108 cfu/mL,只对20种常规抗生素中的5种产生耐药性；对C-5的发酵条件进行优化,该菌优化后的发酵条件为：温度30℃, pH 7.0,盐度2%。结果表明, C-5有望作为一种益生菌应用于海水养殖病害控制。
침대목전해삼양식중수산미생태제제품충단일、시용효과불은정등현상,작자종지과삼(Acaudina molpadioidea)장도분리토저장간균(Enterobacter),사선도1주구유교호길항작용적해양세균C-5,해균대부용혈호균(Vibrio parahaemolyticus)、용조호균(Vibrio alginolyticus)、살해기단포균(Aeromonas salmonicida)등7충세균유길항작용；통과형태관찰、생리생화화16S rDNA기인서렬분석감정위곽장간균(Enterobacter hor-maechei)；통과서충(Artemia)화약민실험검측해균구유안전성,기대서충적72 h반치사농도(LC50)위108 cfu/mL,지대20충상규항생소중적5충산생내약성；대C-5적발효조건진행우화,해균우화후적발효조건위：온도30℃, pH 7.0,염도2%。결과표명, C-5유망작위일충익생균응용우해수양식병해공제。
A probiotic strain of nativeEnterobacternamed as C-5 has been isolated from the intestine ofacaudina molpadioidea in this study. By observing colony morphology, analyzing physiological and biochemical characters, and sequencing of 16S rDNA, C-5 was identified asEnterobacter hormaechei. C-5 showed inhibitory activity against seven pathogens, such asVibrio parahaemolyticus,Vibrio alginolyticus,Aeromonas salmonicida. The secu-rity of C-5 was confirmed by theArtemiachallenge test and drug sensitivity tests. The LC50 of C-5 was found to be 108 cfu/mL, and it was resistant to five antibiotics among twenty used. The optimized fermentation condition of C-5 is: temperature 30℃ , pH 7.0, 2% salinity.Our results showed that C-5 could be used as a kind of probiotic applied to aquaculture disease control.