中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
Chinese Journal of Pathophysiology
2015年
11期
2090-2095
,共6页
昆明山海棠%类风湿性关节炎%基质金属蛋白酶13%白细胞介素
昆明山海棠%類風濕性關節炎%基質金屬蛋白酶13%白細胞介素
곤명산해당%류풍습성관절염%기질금속단백매13%백세포개소
Tripterygium hypoglaucum Hutch%Rheumatoid arthritis%Matrix metalloproteinase-13%Interleu-kins
目的：探讨昆明山海棠（Tripterygium hypoglaucum Hutch，THH）对胶原性关节炎（collagen－induced arthritis，CIA）大鼠的干预作用及可能的机制。方法：SD大鼠50只，随机分为正常组和胶原性关节炎模型制作组，用鸡Ⅱ型胶原诱导制作CIA大鼠模型。将造模成功的36只大鼠随机分为模型组、地塞米松治疗组与THH 200 mg／kg、400 mg／kg干预组。 ELISA法检测CIA大鼠血清和足爪组织中促炎因子IL－12、IL－23和抑炎因子IL－37的含量；HE染色观察各组大鼠足爪组织病理学变化；Western blot法检测足爪组织MMP－13蛋白的表达；荧光标记法检测大鼠足爪组织中MMP－13的活性。结果：较模型组比较， THH 400 mg／kg治疗组大鼠体重下降明显减轻（ P ＜0．01），血清中IL－12和IL－23的水平分别降低了28．31％和41．57％（P＜0．01），足爪组织中IL－12和IL－23的含量分别下降了30．78％和39．46％，而血清和足爪组织中IL－37的水平显著升高了79．43％和75．78％（P＜0．01），足爪皮下组织病理变化明显减轻，足爪组织中MMP－13蛋白表达降低了49．22％（ P＜0．01），且MMP－13活性明显下降。而THH 200 mg／kg治疗组大鼠上述指标无明显改善。结论： THH对CIA大鼠炎症的明显抑制作用可能与其降低促炎因子IL－12和IL－23、增高抑炎因子IL－37含量，抑制炎性细胞浸润及血管增生、下调MMP－13蛋白表达、降低MMP－13活性有关。
目的：探討昆明山海棠（Tripterygium hypoglaucum Hutch，THH）對膠原性關節炎（collagen－induced arthritis，CIA）大鼠的榦預作用及可能的機製。方法：SD大鼠50隻，隨機分為正常組和膠原性關節炎模型製作組，用鷄Ⅱ型膠原誘導製作CIA大鼠模型。將造模成功的36隻大鼠隨機分為模型組、地塞米鬆治療組與THH 200 mg／kg、400 mg／kg榦預組。 ELISA法檢測CIA大鼠血清和足爪組織中促炎因子IL－12、IL－23和抑炎因子IL－37的含量；HE染色觀察各組大鼠足爪組織病理學變化；Western blot法檢測足爪組織MMP－13蛋白的錶達；熒光標記法檢測大鼠足爪組織中MMP－13的活性。結果：較模型組比較， THH 400 mg／kg治療組大鼠體重下降明顯減輕（ P ＜0．01），血清中IL－12和IL－23的水平分彆降低瞭28．31％和41．57％（P＜0．01），足爪組織中IL－12和IL－23的含量分彆下降瞭30．78％和39．46％，而血清和足爪組織中IL－37的水平顯著升高瞭79．43％和75．78％（P＜0．01），足爪皮下組織病理變化明顯減輕，足爪組織中MMP－13蛋白錶達降低瞭49．22％（ P＜0．01），且MMP－13活性明顯下降。而THH 200 mg／kg治療組大鼠上述指標無明顯改善。結論： THH對CIA大鼠炎癥的明顯抑製作用可能與其降低促炎因子IL－12和IL－23、增高抑炎因子IL－37含量，抑製炎性細胞浸潤及血管增生、下調MMP－13蛋白錶達、降低MMP－13活性有關。
목적：탐토곤명산해당（Tripterygium hypoglaucum Hutch，THH）대효원성관절염（collagen－induced arthritis，CIA）대서적간예작용급가능적궤제。방법：SD대서50지，수궤분위정상조화효원성관절염모형제작조，용계Ⅱ형효원유도제작CIA대서모형。장조모성공적36지대서수궤분위모형조、지새미송치료조여THH 200 mg／kg、400 mg／kg간예조。 ELISA법검측CIA대서혈청화족조조직중촉염인자IL－12、IL－23화억염인자IL－37적함량；HE염색관찰각조대서족조조직병이학변화；Western blot법검측족조조직MMP－13단백적표체；형광표기법검측대서족조조직중MMP－13적활성。결과：교모형조비교， THH 400 mg／kg치료조대서체중하강명현감경（ P ＜0．01），혈청중IL－12화IL－23적수평분별강저료28．31％화41．57％（P＜0．01），족조조직중IL－12화IL－23적함량분별하강료30．78％화39．46％，이혈청화족조조직중IL－37적수평현저승고료79．43％화75．78％（P＜0．01），족조피하조직병리변화명현감경，족조조직중MMP－13단백표체강저료49．22％（ P＜0．01），차MMP－13활성명현하강。이THH 200 mg／kg치료조대서상술지표무명현개선。결론： THH대CIA대서염증적명현억제작용가능여기강저촉염인자IL－12화IL－23、증고억염인자IL－37함량，억제염성세포침윤급혈관증생、하조MMP－13단백표체、강저MMP－13활성유관。
[ ABSTRACT] AIM:To investigate the effect of tripterygium hypoglaucum Hutch ( THH) on collagen-induced ar-thritis ( CIA) in rats and its possible mechanism.METHODS:SD rats were randomly divided into normal group and CIA group.The rat model of type II CIA was successfully established and the model rats were randomly divided into 4 different groups:model group, dexamethasone group, THH (200 mg/kg) group, and THH (400 mg/kg) group.The contents of IL-12, IL-23 and IL-37 in the serum and foot paws of the CIA rats were detected by ELISA.The histopathological changes of the skin of the food paws were observed by HE staining.The protein expression of MMP-13 was determined by Western blot.The MMP-13 activity in the foot paws was detected by fluorescence labeling method.RESULTS:Compared with CIA group, THH at dose of 400 mg/kg significantly reduced the weight loss in typeⅡCIA rats ( P<0.01 ) .THH at dose of 400 mg/kg obviously decreased the contents of IL-12 by 28.31%, IL-23 by 41.57%in the serum and IL-12 by 30.78%, IL-23 by 39.46%in the foot paws, while IL-37 was significantly increased by 79.43% in the serum and 75.78% in the foot paws ( P<0.01) .The pathological changes of the subcutaneous tissues were improved by treating with THH (400 mg/kg).The protein expression of MMP-13 was significantly decreased by 31.82%(P<0.01), and the MMP-13 activity was also reduced.THH at dose of 200 mg/kg had no obvious improvement on the above indexes.CONCLUSION:THH has significant inhibitory effect on rat CIA by reducing the content of proinflammatory cytokines IL-12 and IL-23, increasing the content of anti-inflammatory factor IL-37, inhibiting inflammatory cell infiltration and vascular proliferation, and attenuating the protein expression of MMP-13 and MMP-13 activity in rats.
