中国肝脏病杂志（电子版）
中國肝髒病雜誌（電子版）
중국간장병잡지（전자판）
Chinese Journal of Liver Diseases (Electronic Version)
2015年
3期
64-69
,共6页
张毅%余永胜%王洁玲%唐余燕%汤正好%江红%奚敏%臧国庆
張毅%餘永勝%王潔玲%唐餘燕%湯正好%江紅%奚敏%臧國慶
장의%여영성%왕길령%당여연%탕정호%강홍%해민%장국경
肝炎,乙型,慢性%CD4+T细胞%Runx3%Th1细胞%Th2细胞
肝炎,乙型,慢性%CD4+T細胞%Runx3%Th1細胞%Th2細胞
간염,을형,만성%CD4+T세포%Runx3%Th1세포%Th2세포
Chronic hepatitis B%CD4+ T cells%Runx3%Th1 cells%Th2 cells
目的：探讨Runx3基因过表达对慢性乙型肝炎（chronic hepatitis B，CHB）患者Th细胞分化的影响。方法重组慢病毒载体pGC-FU-Runx3与阴性对照慢病毒载体pGC-FU分别转染29例CHB患者外周血CD4+ T细胞，分离培养5 d的细胞培养液上清，应用ELISA检测Th1型细胞因子IFN-γ和Th2型细胞因子IL-4的表达水平；收集培养5 d的CD4+ T细胞，应用实时定量PCR检测转录因子T-bet、GATA3 mRNA的表达水平。结果①与pGC-FU转染组比较，pGC-FU-Runx3转染组Th1型细胞因子IFN-γ的表达水平明显升高（P =0.003）。②与pGC-FU转染组比较，pGC-FU-Runx3转染组Th2型细胞因子IL-4的表达水平明显降低（P =0.007）。③与pGC-FU转染组比较，pGC-FU-Runx3转染组IFN-γ/IL-4比值明显增大（P =0.001）。④与pGC-FU转染组比较，pGC-FU-Runx3转染组T-bet、GATA3 mRNA的表达水平无显著性差异（P均＞0.05），但pGC-FU-Runx3转染组T-bet/GATA3比值较pGC-FU转染组明显增大（P =0.005）。结论 Runx3过表达可促进CHB患者Th1型细胞因子的分泌，抑制Th2型细胞因子分泌，促使CHB患者外周血Th细胞向Th1细胞分化，使其Th1/Th2失平衡得到改善。
目的：探討Runx3基因過錶達對慢性乙型肝炎（chronic hepatitis B，CHB）患者Th細胞分化的影響。方法重組慢病毒載體pGC-FU-Runx3與陰性對照慢病毒載體pGC-FU分彆轉染29例CHB患者外週血CD4+ T細胞，分離培養5 d的細胞培養液上清，應用ELISA檢測Th1型細胞因子IFN-γ和Th2型細胞因子IL-4的錶達水平；收集培養5 d的CD4+ T細胞，應用實時定量PCR檢測轉錄因子T-bet、GATA3 mRNA的錶達水平。結果①與pGC-FU轉染組比較，pGC-FU-Runx3轉染組Th1型細胞因子IFN-γ的錶達水平明顯升高（P =0.003）。②與pGC-FU轉染組比較，pGC-FU-Runx3轉染組Th2型細胞因子IL-4的錶達水平明顯降低（P =0.007）。③與pGC-FU轉染組比較，pGC-FU-Runx3轉染組IFN-γ/IL-4比值明顯增大（P =0.001）。④與pGC-FU轉染組比較，pGC-FU-Runx3轉染組T-bet、GATA3 mRNA的錶達水平無顯著性差異（P均＞0.05），但pGC-FU-Runx3轉染組T-bet/GATA3比值較pGC-FU轉染組明顯增大（P =0.005）。結論 Runx3過錶達可促進CHB患者Th1型細胞因子的分泌，抑製Th2型細胞因子分泌，促使CHB患者外週血Th細胞嚮Th1細胞分化，使其Th1/Th2失平衡得到改善。
목적：탐토Runx3기인과표체대만성을형간염（chronic hepatitis B，CHB）환자Th세포분화적영향。방법중조만병독재체pGC-FU-Runx3여음성대조만병독재체pGC-FU분별전염29례CHB환자외주혈CD4+ T세포，분리배양5 d적세포배양액상청，응용ELISA검측Th1형세포인자IFN-γ화Th2형세포인자IL-4적표체수평；수집배양5 d적CD4+ T세포，응용실시정량PCR검측전록인자T-bet、GATA3 mRNA적표체수평。결과①여pGC-FU전염조비교，pGC-FU-Runx3전염조Th1형세포인자IFN-γ적표체수평명현승고（P =0.003）。②여pGC-FU전염조비교，pGC-FU-Runx3전염조Th2형세포인자IL-4적표체수평명현강저（P =0.007）。③여pGC-FU전염조비교，pGC-FU-Runx3전염조IFN-γ/IL-4비치명현증대（P =0.001）。④여pGC-FU전염조비교，pGC-FU-Runx3전염조T-bet、GATA3 mRNA적표체수평무현저성차이（P균＞0.05），단pGC-FU-Runx3전염조T-bet/GATA3비치교pGC-FU전염조명현증대（P =0.005）。결론 Runx3과표체가촉진CHB환자Th1형세포인자적분비，억제Th2형세포인자분비，촉사CHB환자외주혈Th세포향Th1세포분화，사기Th1/Th2실평형득도개선。
Objective To investigate the effect of lentiviral vector-mediated Runx3 overexpression on Th cell differentiation in patients with chronic hepatitis B (CHB). Methods Peripheral CD4+ T cells derived from 29 CHB patients were transfected with the recombinant lentiviral vector (pGC-FU-Runx3) and the negative control lentiviral vector (pGC-FU) , respectively. Then the cell culture supernatants and the CD4+ T cells were collected on the 5th day. The expression of Th1-type cytokine (IFN-γ) and Th2-type cytokine (IL-4) were measured by ELISA and the expression of T-bet and GATA3 mRNA were assayed by quantitative real-time PCR. Results ①Compared with the pGC-FU transfected group, the expression of IFN-γ in the pGC-FU-Runx3 transfected group significantly increased (P = 0.003). ②Compared with the pGC-FU transfected group, the expression of IL-4 in the pGC-FU-Runx3 transfected group significantly decreased (P = 0.007).③Compared with the pGC-FU transfected group, the ratio of IFN-γ/IL-4 in the pGC-FU-Runx3 transfected group significantly increased (P = 0.001). ④There was no difference in T-bet and GATA3 mRNA expression between the pGC-FU transfected group and the pGC-FU-Runx3 transfected group (both P > 0.05). However, compared with the pGC-FU transfected group, the ratio of T-bet/GATA3 in the pGC-FU-Runx3 transfected group significantly increased (P = 0.005). Conclusions Runx3 overexpression can promote the secretion of Th1-type cytokines and inhibit the secretion of Th2-type cytokines in CHB patients. It can also induce Th cell differentiating into Th1 cell lineage and improve the Th1/Th2 imbalance in CHB patients.
