目的 观察子午沙鼠骨组织细粒棘球蚴病放射治疗后转归情况.方法 建立子午沙鼠骨细粒棘球蚴病动物模型240只,按体质量采用随机数字表法分为3组:对照组、40 Gy/5次组、50 Gy/5次组,每组8C只,雌雄各半.对照组沙鼠未进行干预处理,40 Gy/5次和50 Gy/5次组采用6WV-X线定位照射治疗,5次连续放射,间隔2天后再重复放射5次.放疗结束后3、6个月时,观察子午沙鼠死亡和病变部位破溃、感染情况;每组选取存活沙鼠15只处死,观察计算囊液内头节死亡数并检测蛋白质、钙离子浓度,测量病变部位最大直径和细粒棘球蚴囊湿重;显微镜下观察病变部位骨骼破坏、重建情况.结果 放疗结束后3、6个月时,随着放射剂量升高,沙鼠死亡数量明显减少(x2=10.4、17.4,P均＜0.05);沙鼠病变部位破溃、感染情况明显降低(x2=6.0、10.1,P均＜0.05);囊液内头节死亡例数明显升高[3个月:(22.4±3.1)、(95.0±5.2)、(136.0±5.4)个;6个月:(23.2±2.2)、(98.2±4.6)、(169.3±7.0)个;F=2 252.5、3 220.3,P均＜0.05];囊液蛋白质含量、钙离子浓度明显改变[3个月:(1.059±0.056)、(0.733±0.051)、(0.571±0.043)g/L和(2.802±0.157)、(3.056±0.060)、(3.546±0.135)mmol/L;6个月:(1.088±0.043)、(0.753±0.034)、(0.340±0.032)g/L和(2.804±0.019)、(3.068±0.052)、(3.886±0.046) mmol/L;F=366.0、138.9和1 550.5、2 727.3,P均＜0.05];病变部位最大直径明显减小[3个月:(2.38±0.14)、(1.69±0.05)、(1.40±0.09)cm;6个月:(2.65±0.05)、(1.69±0.03)、(1.03±0.06)cm;F=372.5、3 846.1,P均＜0.05];细粒棘球蚴囊湿重明显减小[3个月:(3.47±0.11)、(2.54±0.12)、(1.46±0.07)g;6个月:(3.75±0.31)、(2.55±0.08)、(1.02±0.20)g;F=1 475.6、608.0,P均＜0.05].随着时间延长,对照组及40 Gy/5次组沙鼠死亡数量明显升高(x2=4.3、4.6,P均＜0.05),而50 Gy/5次组未见明显改变(x2=1.1,P＞0.05);对照组及40 Gy/5次组沙鼠病变部位破溃、感染情况明显增加(x2=5.5、4.3,P均＜0.05),而50 Gy/5次组未见明显改变(x2=0.3,P＞0.05);50 Gy/5次组囊液内头节死亡例数明显升高,囊液蛋白质含量、钙离子浓度明显改变(F=212.6、271.8、84.7,P均＜0.05);对照组病变部位最大直径明显增加(F=47.1,P＜0.05),50Gy/5次组明显减小(F=188.3,P＜0.05);对照组细粒棘球蚴囊湿重明显增加(F=10.7,P＜0.05),50 Gy/5次组明显减小(F=68.5,P＜0.05).光镜下,随着放射剂量升高,病变部位骨基质及陷窝内细胞损伤情况逐渐加重;随着时间延长,对照组内骨陷窝内细胞少量死亡,而40Gy/5次组及50 Gy/5次组骨基质及骨陷窝内细胞都有变性坏死和部分修复.结论 经合适剂量(50Gy/5次)的放射线治疗,子午沙鼠细粒棘球蚴病的远期效果较好.
目的 觀察子午沙鼠骨組織細粒棘毬蚴病放射治療後轉歸情況.方法 建立子午沙鼠骨細粒棘毬蚴病動物模型240隻,按體質量採用隨機數字錶法分為3組:對照組、40 Gy/5次組、50 Gy/5次組,每組8C隻,雌雄各半.對照組沙鼠未進行榦預處理,40 Gy/5次和50 Gy/5次組採用6WV-X線定位照射治療,5次連續放射,間隔2天後再重複放射5次.放療結束後3、6箇月時,觀察子午沙鼠死亡和病變部位破潰、感染情況;每組選取存活沙鼠15隻處死,觀察計算囊液內頭節死亡數併檢測蛋白質、鈣離子濃度,測量病變部位最大直徑和細粒棘毬蚴囊濕重;顯微鏡下觀察病變部位骨骼破壞、重建情況.結果 放療結束後3、6箇月時,隨著放射劑量升高,沙鼠死亡數量明顯減少(x2=10.4、17.4,P均＜0.05);沙鼠病變部位破潰、感染情況明顯降低(x2=6.0、10.1,P均＜0.05);囊液內頭節死亡例數明顯升高[3箇月:(22.4±3.1)、(95.0±5.2)、(136.0±5.4)箇;6箇月:(23.2±2.2)、(98.2±4.6)、(169.3±7.0)箇;F=2 252.5、3 220.3,P均＜0.05];囊液蛋白質含量、鈣離子濃度明顯改變[3箇月:(1.059±0.056)、(0.733±0.051)、(0.571±0.043)g/L和(2.802±0.157)、(3.056±0.060)、(3.546±0.135)mmol/L;6箇月:(1.088±0.043)、(0.753±0.034)、(0.340±0.032)g/L和(2.804±0.019)、(3.068±0.052)、(3.886±0.046) mmol/L;F=366.0、138.9和1 550.5、2 727.3,P均＜0.05];病變部位最大直徑明顯減小[3箇月:(2.38±0.14)、(1.69±0.05)、(1.40±0.09)cm;6箇月:(2.65±0.05)、(1.69±0.03)、(1.03±0.06)cm;F=372.5、3 846.1,P均＜0.05];細粒棘毬蚴囊濕重明顯減小[3箇月:(3.47±0.11)、(2.54±0.12)、(1.46±0.07)g;6箇月:(3.75±0.31)、(2.55±0.08)、(1.02±0.20)g;F=1 475.6、608.0,P均＜0.05].隨著時間延長,對照組及40 Gy/5次組沙鼠死亡數量明顯升高(x2=4.3、4.6,P均＜0.05),而50 Gy/5次組未見明顯改變(x2=1.1,P＞0.05);對照組及40 Gy/5次組沙鼠病變部位破潰、感染情況明顯增加(x2=5.5、4.3,P均＜0.05),而50 Gy/5次組未見明顯改變(x2=0.3,P＞0.05);50 Gy/5次組囊液內頭節死亡例數明顯升高,囊液蛋白質含量、鈣離子濃度明顯改變(F=212.6、271.8、84.7,P均＜0.05);對照組病變部位最大直徑明顯增加(F=47.1,P＜0.05),50Gy/5次組明顯減小(F=188.3,P＜0.05);對照組細粒棘毬蚴囊濕重明顯增加(F=10.7,P＜0.05),50 Gy/5次組明顯減小(F=68.5,P＜0.05).光鏡下,隨著放射劑量升高,病變部位骨基質及陷窩內細胞損傷情況逐漸加重;隨著時間延長,對照組內骨陷窩內細胞少量死亡,而40Gy/5次組及50 Gy/5次組骨基質及骨陷窩內細胞都有變性壞死和部分脩複.結論 經閤適劑量(50Gy/5次)的放射線治療,子午沙鼠細粒棘毬蚴病的遠期效果較好.
목적 관찰자오사서골조직세립극구유병방사치료후전귀정황.방법 건립자오사서골세립극구유병동물모형240지,안체질량채용수궤수자표법분위3조:대조조、40 Gy/5차조、50 Gy/5차조,매조8C지,자웅각반.대조조사서미진행간예처리,40 Gy/5차화50 Gy/5차조채용6WV-X선정위조사치료,5차련속방사,간격2천후재중복방사5차.방료결속후3、6개월시,관찰자오사서사망화병변부위파궤、감염정황;매조선취존활사서15지처사,관찰계산낭액내두절사망수병검측단백질、개리자농도,측량병변부위최대직경화세립극구유낭습중;현미경하관찰병변부위골격파배、중건정황.결과 방료결속후3、6개월시,수착방사제량승고,사서사망수량명현감소(x2=10.4、17.4,P균＜0.05);사서병변부위파궤、감염정황명현강저(x2=6.0、10.1,P균＜0.05);낭액내두절사망례수명현승고[3개월:(22.4±3.1)、(95.0±5.2)、(136.0±5.4)개;6개월:(23.2±2.2)、(98.2±4.6)、(169.3±7.0)개;F=2 252.5、3 220.3,P균＜0.05];낭액단백질함량、개리자농도명현개변[3개월:(1.059±0.056)、(0.733±0.051)、(0.571±0.043)g/L화(2.802±0.157)、(3.056±0.060)、(3.546±0.135)mmol/L;6개월:(1.088±0.043)、(0.753±0.034)、(0.340±0.032)g/L화(2.804±0.019)、(3.068±0.052)、(3.886±0.046) mmol/L;F=366.0、138.9화1 550.5、2 727.3,P균＜0.05];병변부위최대직경명현감소[3개월:(2.38±0.14)、(1.69±0.05)、(1.40±0.09)cm;6개월:(2.65±0.05)、(1.69±0.03)、(1.03±0.06)cm;F=372.5、3 846.1,P균＜0.05];세립극구유낭습중명현감소[3개월:(3.47±0.11)、(2.54±0.12)、(1.46±0.07)g;6개월:(3.75±0.31)、(2.55±0.08)、(1.02±0.20)g;F=1 475.6、608.0,P균＜0.05].수착시간연장,대조조급40 Gy/5차조사서사망수량명현승고(x2=4.3、4.6,P균＜0.05),이50 Gy/5차조미견명현개변(x2=1.1,P＞0.05);대조조급40 Gy/5차조사서병변부위파궤、감염정황명현증가(x2=5.5、4.3,P균＜0.05),이50 Gy/5차조미견명현개변(x2=0.3,P＞0.05);50 Gy/5차조낭액내두절사망례수명현승고,낭액단백질함량、개리자농도명현개변(F=212.6、271.8、84.7,P균＜0.05);대조조병변부위최대직경명현증가(F=47.1,P＜0.05),50Gy/5차조명현감소(F=188.3,P＜0.05);대조조세립극구유낭습중명현증가(F=10.7,P＜0.05),50 Gy/5차조명현감소(F=68.5,P＜0.05).광경하,수착방사제량승고,병변부위골기질급함와내세포손상정황축점가중;수착시간연장,대조조내골함와내세포소량사망,이40Gy/5차조급50 Gy/5차조골기질급골함와내세포도유변성배사화부분수복.결론 경합괄제량(50Gy/5차)적방사선치료,자오사서세립극구유병적원기효과교호.
Objective According to radiation therapy (6WV-X line) on experimental gerbils which were successfully infected by echinococcus granulosus,the outcomes of bone hydatid disease after radiation therapy were studied.Methods Totally 240 gerbil models that were infected bone hydatid disease,were randomly divided into three groups (each group was further divided into three-month and six-month groups,40 gerbils per group),one group as a control group,the 40 Gy/5 times and 50 Gy/5 times groups were given 6WV-X line radiation therapy.After 5 consecutive radiation therapies,stopped for two days and then repeated for five times.At the end of three and six months after radiotherapy,the rate of death and the ulceration or infection of the lesions was compared.Fifteen gerbils from each group were randomly selected to observe the deaths of scolex,protein and calcium concentration changes,the maximum diameter changes of the lesions,the changes of hydatid cyst wet weight and the rate of suppressing capsule,the bone destruction,and rebuilding situation of lesions under a microscope.Results At the end of three and six months after radiation therapy,with increasing dosage,the deaths decreased significantly (x2 =10.4,17.4,all P ＜ 0.05);the ulceration or infection of the lesions decreased significantly (x2 =6.0,10.1,all P ＜ 0.05);the mortality rate of scolex increased [3 month:(22.4 ± 3.1),(95.0 ± 5.2),(136.0 ± 5.4);6 month:(23.2 ± 2.2),(98.2 ± 4.6),(169.3 ± 7.0);F =2 252.5,3 220.3,all P ＜ 0.05];the concentration of protein and the calcium ion were changed significantly [3 month:(1.059 ± 0.056),(0.733 ± 0.051),(0.571 ± 0.043)g/L and (2.802 ± 0.157),(3.056 ± 0.060),(3.546 ± 0.135)mmol/L;6 month:(1.088 ± 0.043),(0.753 ± 0.034),(0.340 ± 0.032)g/L and (2.804 ± 0.019),(3.068 ± 0.052),(3.886 ± 0.046)mmol/L;F =366.0,138.9 and 1 550.5,2 727.3,all P ＜ 0.05];the maximum diameters of the lesions reduced significantly [3 month:(2.38 ± 0.14),(1.69 ± 0.05),(1.40 ± 0.09)cm;6 month:(2.65 ± 0.05),(1.69 ± 0.03),(1.03 ± 0.06)cm;F =372.5,3 846.1,all P ＜ 0.05];the hydatid cyst wet weight decreased significantly [3 month:(3.47 ± 0.11),(2.54 ± 0.12),(1.46 ± 0.07)g;6 month:(3.75 ± 0.31),(2.55 ± 0.08),(1.02 ± 0.20)g;F =1 475.6,608.0,all P ＜ 0.05].In the same group with time went on,in the control and 40 Gy/5 times group,the deaths gradually increased (x2 =4.3,4.6,all P ＜ 0.05),but in the 50 Gy/5 times group,the deaths was not significantly increased (x2 =1.1,P ＞ 0.05);in the control and 40 Gy/5 times group,the ulceration or infection of the lesions gradually increased (x2 =5.5,4.3,all P ＜ 0.05),but in the 50 Gy/5 times group,the ulceration or infection of the lesions did not change significantly (x2 =0.3,P ＞ 0.05);in the 50 Gy/5 times group,the mortality rate of scolex was significantly increased (F =212.6,P ＜ 0.05);in 50 Gy/5 times group,the protein (calcium) concentration decreased (increased) significantly (F =271.8,84.7,all P ＜ 0.05);the maximum diameters of the lesions increased gradually in the control group (F =47.1,P ＜ 0.05),in 50 Gy/5 times group,the maximum diameters of the lesions decreased gradually (F =188.3,P ＜ 0.05);in the control group,hydatid cyst wet weight increased significantly (F =10.7,P ＜ 0.05),in the 50 Gy/5 times group,hydatid cyst wet weight was significantly reduced (F =68.5,P ＜ 0.05);with increasing dosage,the damage of the bone matrix and the cells in lacunae of the lesions gradually increased,in the same group with time went on,in the control group,a few amount of bone cells in lacuna died,and in 40 Gy/5 times and 50 Gy/5 times groups,the bone matrix and bone cells were partially repaired.Conclusion The long-term effects of appropriate dosage (50 Gy/5 times) radiation on experiments hydatid diseased gerbils are affirmed,but it is still need a clinical validation.
