CAJ | 학술논문

目的：研究甘氨酸受体α1亚基（GlyRα1）在乳鼠心肌细胞中的表达以及脂多糖（LPS）、缺氧／复氧（ H／R）、异丙肾上腺素（ ISO）和高糖（ HG）对其表达的影响。方法：体外培养乳鼠心肌细胞，Western blotting方法检测心肌细胞上GlyRα1的表达；心肌细胞分别用LPS、H／R、ISO以及HG处理24 h，采用CCK－8试剂检测细胞活力， Western blotting方法检测心肌细胞上GlyRα1的表达。结果： Western blotting 方法检测到乳鼠心肌细胞上GlyRα1的表达；LPS（20 mg／L）、ISO（100μmol／L）以及HG（25mmol／L）处理心肌细胞24 h与心肌细胞H／R 3 h对心肌细胞存活率无明显影响；LPS组、H／R 3 h组以及ISO组心肌细胞上GlyRα1表达均高于对照组（ P＜0．01），而HG组心肌细胞上GlyRα1表达低于对照组（P＜0．01）。结论：乳鼠心肌细胞上存在GlyRα1，并且一定浓度的LPS、ISO与一定时间的H／R均可上调乳鼠心肌细胞GlyRα1的表达，而HG可下调乳鼠心肌细胞GlyRα1的表达。
목적：연구감안산수체α1아기（GlyRα1）재유서심기세포중적표체이급지다당（LPS）、결양／복양（ H／R）、이병신상선소（ ISO）화고당（ HG）대기표체적영향。방법：체외배양유서심기세포，Western blotting방법검측심기세포상GlyRα1적표체；심기세포분별용LPS、H／R、ISO이급HG처리24 h，채용CCK－8시제검측세포활력， Western blotting방법검측심기세포상GlyRα1적표체。결과： Western blotting 방법검측도유서심기세포상GlyRα1적표체；LPS（20 mg／L）、ISO（100μmol／L）이급HG（25mmol／L）처리심기세포24 h여심기세포H／R 3 h대심기세포존활솔무명현영향；LPS조、H／R 3 h조이급ISO조심기세포상GlyRα1표체균고우대조조（ P＜0．01），이HG조심기세포상GlyRα1표체저우대조조（P＜0．01）。결론：유서심기세포상존재GlyRα1，병차일정농도적LPS、ISO여일정시간적H／R균가상조유서심기세포GlyRα1적표체，이HG가하조유서심기세포GlyRα1적표체。
AIM:To study the expression of glycine receptorα1 subunit in neonatal rat myocardial cells and to investigate the effect of lipopolysaccharide (LPS), hypoxia/reoxygenation, isoproterenol (ISO) and high concentration of glucose (HG) on the expression of glycine receptorα1 subunit in the neonatal rat myocardial cells.METHODS:Neonatal rat myocardial cells were cultured in vitro.The expression of glycine receptorα1 subunit was detected by Western blotting. The neonatal rat myocardial cells were treated with LPS (20 mg/L), ISO (100 μmol/L) or high concentration of glucose (25 mmol/L) for 24 h, or were exposed to hypoxia for 3 h followed by reoxygenation for 3 h.Subsequently, the cell viabil-ity was measured by CCK-8 assay, and the expression of glycine receptorα1 subunit was determined by Western blotting. RESULTS:The expression of glycine receptor α1 subunit in the neonatal rat myocardial cells was positively detectable by Western blotting.Compared with control group, no significant difference of the cell viability ( P>0.05) in LPS group, ISO group, hypoxia/reoxygenation group and HG group was observed.The expression of glycine receptor α1 subunit was in-creased (P<0.01) in LPS group, ISO group and hypoxia/reoxygenatio group, but decreased (P<0.01) in HG group. CONCLUSION:Glycine receptorα1 subunit exists in the neonatal rat myocardial cells.A certain concentration of LPS or ISO, or hypoxia/reoxygenation for a certain period upregulate the expression of glycine receptorα1 subunit, but HG down-regulates the expression of glycine receptor α1 subunit in cultured neonatal rat myocardial cells.