CAJ | 학술논문

目的：探讨抑癌基因ARHI在急性髓细胞白血病中的表达情况，同时探索其对U937细胞生长的影响。方法：RT－PCR方法检测急性髓细胞白血病细胞株、人胚肾细胞293FT、白血病原代细胞以及健康志愿者血细胞中ARHI mRNA的表达情况。构建高表达ARHI的MSCV－IRES－GFP－ARHI 质粒转染U937细胞，MTT法连续8 d检测细胞活性绘制生长曲线。新鲜培养基重悬U937细胞24 h后，采用流式细胞术检测细胞周期以及细胞凋亡。结果：293 FT细胞、健康志愿者血细胞中均检测到ARHI mRNA表达，而白血病细胞株以及白血病患者原代细胞中未检测到该基因的表达。生长曲线显示高表达ARHI基因的U937（ U937－ARHI）细胞在第6～8天细胞活力低于对照（U937－GFP）组。高表达ARHI的U937细胞G2／M期细胞比例及细胞凋亡率均高于对照组（P＜0．05）。结论：ARHI在急性髓系白血病细胞中的表达减低；高表达ARHI基因能抑制U937细胞生长、阻滞其细胞周期在G2／M期并促进细胞凋亡。
목적：탐토억암기인ARHI재급성수세포백혈병중적표체정황，동시탐색기대U937세포생장적영향。방법：RT－PCR방법검측급성수세포백혈병세포주、인배신세포293FT、백혈병원대세포이급건강지원자혈세포중ARHI mRNA적표체정황。구건고표체ARHI적MSCV－IRES－GFP－ARHI 질립전염U937세포，MTT법련속8 d검측세포활성회제생장곡선。신선배양기중현U937세포24 h후，채용류식세포술검측세포주기이급세포조망。결과：293 FT세포、건강지원자혈세포중균검측도ARHI mRNA표체，이백혈병세포주이급백혈병환자원대세포중미검측도해기인적표체。생장곡선현시고표체ARHI기인적U937（ U937－ARHI）세포재제6～8천세포활력저우대조（U937－GFP）조。고표체ARHI적U937세포G2／M기세포비례급세포조망솔균고우대조조（P＜0．05）。결론：ARHI재급성수계백혈병세포중적표체감저；고표체ARHI기인능억제U937세포생장、조체기세포주기재G2／M기병촉진세포조망。
AIM:To investigate the expression of aplasia rashomolog member I ( ARHI) gene in acute myeloid leukemia cells (AML) and to study the effects of ARHI on the growth of AML cell line U937.METHODS:The mRNA ex-pression of ARHI in AML cells, 293FT cells, AML primary cells and healthy volunteer blood cells were detected by RT-PCR.After transfection with the MSCV-IRES-GFP-ARHI plasmid to the U937 cells, the growth curve was analyzed by MTT assay.U937 cells were re-suspended by fresh medium and cultured for 24 h, then the cell cycle distribution and ap-optotic rate were determined.RESULTS:The mRNA of ARHI was positively detectable in 293FT cells and healthy volun-teer blood cells instead of AML cell line and AML primary cells.The growth curve showed that cell viability in U937 cells with high expression of ARHI (U937-ARHI) was lower than that in the control cells (U937-GFP) on 6th~8th day.The ratio of G2/M phase and apoptotic rate in the U937-ARHI cells were increased compare with control group ( P<0.05 ) . CONCLUSION:The mRNA level of ARHI is low in AML cells.High expression of ARHI gene in U937 cells inhibits cell growth, arrests the cells at G2/M phase and induces apoptosis.