中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
11期
808-812
,共5页
孙宗祥%姜勇波%张丽娜%高晓丽
孫宗祥%薑勇波%張麗娜%高曉麗
손종상%강용파%장려나%고효려
荧光定量PCR%布鲁杆菌病%DNA
熒光定量PCR%佈魯桿菌病%DNA
형광정량PCR%포로간균병%DNA
Real-time PCR%Brucellosis%DNA
目的 评估荧光定量PCR方法检测人群血样中布鲁杆菌DNA的快速性和特异性.方法 以bcsp31基因作为荧光定量PCR的检测目标,利用15种已知布鲁杆菌菌株和41种非布鲁杆菌菌株DNA评价荧光定量PCR方法检测布鲁杆菌的种属特异性.以2012年哈尔滨市疾病预防控制中心布鲁杆菌病监测人群结果中的17份布鲁杆菌阳性血液为样本,另选取30名健康者和30名非布鲁杆菌病(布病)患者血样作为对照,评价荧光定量PCR方法应用于检测人群血样布鲁杆菌的相应灵敏度和特异性.结果 荧光定量-PCR结果显示15个布鲁杆菌中bcsp31基因检测均为阳性,41个非布鲁杆菌种均未检出bcsp31基因.在17名布病患者中,11份血液样本bcsp31基因扩增信号阳性,灵敏度为64.7％(11/17);60名对照的血清均显示阴性结果,相应灵敏度为100.0％.结论 与传统方法相比,荧光定量PCR方法具有快速、特异等优点,适用于布病确诊.
目的 評估熒光定量PCR方法檢測人群血樣中佈魯桿菌DNA的快速性和特異性.方法 以bcsp31基因作為熒光定量PCR的檢測目標,利用15種已知佈魯桿菌菌株和41種非佈魯桿菌菌株DNA評價熒光定量PCR方法檢測佈魯桿菌的種屬特異性.以2012年哈爾濱市疾病預防控製中心佈魯桿菌病鑑測人群結果中的17份佈魯桿菌暘性血液為樣本,另選取30名健康者和30名非佈魯桿菌病(佈病)患者血樣作為對照,評價熒光定量PCR方法應用于檢測人群血樣佈魯桿菌的相應靈敏度和特異性.結果 熒光定量-PCR結果顯示15箇佈魯桿菌中bcsp31基因檢測均為暘性,41箇非佈魯桿菌種均未檢齣bcsp31基因.在17名佈病患者中,11份血液樣本bcsp31基因擴增信號暘性,靈敏度為64.7％(11/17);60名對照的血清均顯示陰性結果,相應靈敏度為100.0％.結論 與傳統方法相比,熒光定量PCR方法具有快速、特異等優點,適用于佈病確診.
목적 평고형광정량PCR방법검측인군혈양중포로간균DNA적쾌속성화특이성.방법 이bcsp31기인작위형광정량PCR적검측목표,이용15충이지포로간균균주화41충비포로간균균주DNA평개형광정량PCR방법검측포로간균적충속특이성.이2012년합이빈시질병예방공제중심포로간균병감측인군결과중적17빈포로간균양성혈액위양본,령선취30명건강자화30명비포로간균병(포병)환자혈양작위대조,평개형광정량PCR방법응용우검측인군혈양포로간균적상응령민도화특이성.결과 형광정량-PCR결과현시15개포로간균중bcsp31기인검측균위양성,41개비포로간균충균미검출bcsp31기인.재17명포병환자중,11빈혈액양본bcsp31기인확증신호양성,령민도위64.7％(11/17);60명대조적혈청균현시음성결과,상응령민도위100.0％.결론 여전통방법상비,형광정량PCR방법구유쾌속、특이등우점,괄용우포병학진.
Objective In this article we evaluated the sensitivities and specificities of real-time PCR assay for diagnosis of human brucellosis.Methods The species selectivity and specificity of real-time PCR were evaluated by direct amplification of a 169 bp portion of bcsp31 gene from 15 Brucella strains and 41 non-Brucella strains.According to the monitoring results of 2012 Harbin brucellosis,17 brucellosis patients and 30 health people were selected to collect their serum samples for assessing the sensitivity of real-time PCR,and additional 30 nonbrucellosis patients serum samples were as controls.Results The species selectivity and specificity of our realtime PCR method were evaluated by using genomic DNA from 15 Brucella strains and 41 non-Brucella strains.There were 11 sera with positive amplification signals among the 17 culture-proven brucellosis patients,the sensitivity was 64.7％(11/17).Whereas,the results of sera from the 60 control patients were all negative,corresponding to a specificity of 100.0％.Conclusion The results indicate that real-time PCR is well suitable for confirmation of brucellosis cases.
