CAJ | 학술논문

目的：通过研究表皮生长因子及其受体（EGF／EGFR）调控基质金属蛋白酶-2（MMP-2）在腺样囊性癌细胞中的表达，为进一步探讨MMP-2在腺样囊性癌发生、发展、转移过程的机制奠定基础。方法应用免疫组织化学SP法检测正常涎腺及腺样囊性癌组织中EGFR和MMP-2的表达分布情况；利用定时定量RT-PCR法检测不同剂量的EGF调控MMP-2 mRNA在腺样囊性癌细胞中的表达水平，分析EGFR阻断剂作用下EGF调控MMP-2 mRNA的表达水平；最后利用免疫印迹Western blot法检测EGF调控下MMP-2在腺样囊性癌细胞中蛋白表达水平。结果免疫组化结果显示EGFR和MMP-2在腺样囊性癌组织中呈阳性表达，在正常腮腺组织中成阴性表达；定时定量RT-PCR法分析得出EGF上调MMP-2 mRNA在腺样囊性癌细胞中的表达，在20μg／L的剂量时上调作用最为显著，同时发现EGFR阻断剂可有效抑制EGF的上调作用；Western blot法验证了MMP-2蛋白在腺样囊性癌细胞的表达水平受到EGF的调节。结论表皮生长因子EGF可以促进MMP-2在腺样囊性癌细胞中的表达水平，可为临床治疗腺样囊性癌提供新的理论指导。
목적：통과연구표피생장인자급기수체（EGF／EGFR）조공기질금속단백매-2（MMP-2）재선양낭성암세포중적표체，위진일보탐토MMP-2재선양낭성암발생、발전、전이과정적궤제전정기출。방법응용면역조직화학SP법검측정상연선급선양낭성암조직중EGFR화MMP-2적표체분포정황；이용정시정량RT-PCR법검측불동제량적EGF조공MMP-2 mRNA재선양낭성암세포중적표체수평，분석EGFR조단제작용하EGF조공MMP-2 mRNA적표체수평；최후이용면역인적Western blot법검측EGF조공하MMP-2재선양낭성암세포중단백표체수평。결과면역조화결과현시EGFR화MMP-2재선양낭성암조직중정양성표체，재정상시선조직중성음성표체；정시정량RT-PCR법분석득출EGF상조MMP-2 mRNA재선양낭성암세포중적표체，재20μg／L적제량시상조작용최위현저，동시발현EGFR조단제가유효억제EGF적상조작용；Western blot법험증료MMP-2단백재선양낭성암세포적표체수평수도EGF적조절。결론표피생장인자EGF가이촉진MMP-2재선양낭성암세포중적표체수평，가위림상치료선양낭성암제공신적이론지도。
[ ABSTRACT] Objective To research the regulation of matrix metalloproteinases-2( MMP-2) in adenoid cystic carcinoma which in-duced by EGF/EGFR,and to further explore the mechanisms of the MMP-2 in occurrence,development and metastasis of the adenoid cystic carcinoma.Methods First of all immunohistochemistry was used to observe the expression and distribute of EGFR and MMP-2 in the normal salivary gland and adenoid cystic carcinoma;the real time RT-PCR was used to observe the expression of MMP-2 mRNA in adenoid cystic carcinoma induced by different doses of EGF,then we analysized the expression of MMP-2 mRNA induced by EGF which acted by EGFR in-hibitor;at the last we observed that EGF induced the expression of MMP-2 protein in adenoid cystic carcinoma by western blot.Results Im-munohistochemistry showed that EGFR and MMP-2 have the positive expressions in adenoid cystic carcinoma,while have the negative expres-sions in normal salivary gland;the real time RT-PCR showed that EGF can increase the expression of MMP-2 in adenoid cystic carcinoma,es-pecially in 20μg/L.It also was found that EGFR inhibitor can suppress the increased expression of MMP-2 induced by EGF.The western blot was used to verify that the expression of MMP-2 protein in adenoid cystic carcinoma was induced by EGF.Conclusion The expression of MMP-2 in adenoid cystic carcinoma was induced by EGF,the new theoretical guidance was taken to the clinical treatment of adenoid cystic carcinoma.