中国医学创新
中國醫學創新
중국의학창신
Medical Innovation of China
2015年
30期
14-16
,共3页
赵腾%陈海龙%刘晶瑶%孙莉%周春奎
趙騰%陳海龍%劉晶瑤%孫莉%週春奎
조등%진해룡%류정요%손리%주춘규
慢性脑缺血%血管性痴呆%神经束蛋白155
慢性腦缺血%血管性癡呆%神經束蛋白155
만성뇌결혈%혈관성치태%신경속단백155
Chronic ischemia%Vascular dementi%Neurofascin155
目的:探讨慢性脑缺血致血管性痴呆与神经束蛋白155的相关性.方法:2013年12月-2014年5月期间选用Wistar大鼠40只,随机分为模型组24只、对照组12只.模型组大鼠使用双侧颈总动脉永久结扎法(2VO)制备慢性前脑缺血致血管性痴呆动物模型,对照组仅手术而不行双侧颈总动脉结扎.使用Morris水迷宫对各组大鼠手术前和手术后的记忆行为数据进行检测.比较手术前后及各组间逃避潜伏期的差异,明确模型组大鼠是否达到痴呆.术后30 d和60 d取大鼠脑组织并制成匀浆,使用ELISA检测各组NF155的表达情况.结果:造模前模型组小鼠逃避潜伏期与假手术对照组比较差异无统计学意义(P>0.05).造模后,术后30 d组第6天开始,逃避潜伏期均明显长于假手术对照组,差异均有统计学意义(t=2.667、6.812、8.743、10.775;P=0.014、0.000、0.000、0.000);术后60 d组第5天开始,逃避潜伏期均明显长于假手术对照组,差异均有统计学意义(t=3.016、4.137、7.562、7.910、12.686;P=0.006、0.000、0.000、0.000、0.000).经ELISA检测显示模型30 d组及60 d组大鼠NF155浓度分别为(48.27±7.32)、(37.65±6.88)ng/mL,与假手术对照组(85.07±6.75)ng/mL比较,差异均有统计学意义(P<0.05),说明模型组大鼠脑内NF155的含量较对照组明显下降.结论:脑内NF155含量的变化有可能参与了慢性缺血所致血管性痴呆的发生.
目的:探討慢性腦缺血緻血管性癡呆與神經束蛋白155的相關性.方法:2013年12月-2014年5月期間選用Wistar大鼠40隻,隨機分為模型組24隻、對照組12隻.模型組大鼠使用雙側頸總動脈永久結扎法(2VO)製備慢性前腦缺血緻血管性癡呆動物模型,對照組僅手術而不行雙側頸總動脈結扎.使用Morris水迷宮對各組大鼠手術前和手術後的記憶行為數據進行檢測.比較手術前後及各組間逃避潛伏期的差異,明確模型組大鼠是否達到癡呆.術後30 d和60 d取大鼠腦組織併製成勻漿,使用ELISA檢測各組NF155的錶達情況.結果:造模前模型組小鼠逃避潛伏期與假手術對照組比較差異無統計學意義(P>0.05).造模後,術後30 d組第6天開始,逃避潛伏期均明顯長于假手術對照組,差異均有統計學意義(t=2.667、6.812、8.743、10.775;P=0.014、0.000、0.000、0.000);術後60 d組第5天開始,逃避潛伏期均明顯長于假手術對照組,差異均有統計學意義(t=3.016、4.137、7.562、7.910、12.686;P=0.006、0.000、0.000、0.000、0.000).經ELISA檢測顯示模型30 d組及60 d組大鼠NF155濃度分彆為(48.27±7.32)、(37.65±6.88)ng/mL,與假手術對照組(85.07±6.75)ng/mL比較,差異均有統計學意義(P<0.05),說明模型組大鼠腦內NF155的含量較對照組明顯下降.結論:腦內NF155含量的變化有可能參與瞭慢性缺血所緻血管性癡呆的髮生.
목적:탐토만성뇌결혈치혈관성치태여신경속단백155적상관성.방법:2013년12월-2014년5월기간선용Wistar대서40지,수궤분위모형조24지、대조조12지.모형조대서사용쌍측경총동맥영구결찰법(2VO)제비만성전뇌결혈치혈관성치태동물모형,대조조부수술이불행쌍측경총동맥결찰.사용Morris수미궁대각조대서수술전화수술후적기억행위수거진행검측.비교수술전후급각조간도피잠복기적차이,명학모형조대서시부체도치태.술후30 d화60 d취대서뇌조직병제성균장,사용ELISA검측각조NF155적표체정황.결과:조모전모형조소서도피잠복기여가수술대조조비교차이무통계학의의(P>0.05).조모후,술후30 d조제6천개시,도피잠복기균명현장우가수술대조조,차이균유통계학의의(t=2.667、6.812、8.743、10.775;P=0.014、0.000、0.000、0.000);술후60 d조제5천개시,도피잠복기균명현장우가수술대조조,차이균유통계학의의(t=3.016、4.137、7.562、7.910、12.686;P=0.006、0.000、0.000、0.000、0.000).경ELISA검측현시모형30 d조급60 d조대서NF155농도분별위(48.27±7.32)、(37.65±6.88)ng/mL,여가수술대조조(85.07±6.75)ng/mL비교,차이균유통계학의의(P<0.05),설명모형조대서뇌내NF155적함량교대조조명현하강.결론:뇌내NF155함량적변화유가능삼여료만성결혈소치혈관성치태적발생.
Objective:To investigate the correlation between vascular dementia by chronic ischemia and NF155. Method:40 male wistar rats were randomly divided into model group of 24 cases and the control group of 12 cases from December 2013 to May 2014.Rats in the model group were treated with permanent bilateral carotid artery ligation (2VO) to prepare chronic forebrain ischemia induced animal model of vascular dementia, and the rats in the control group just treated surgery.Morris water maze was given in each group before and after operation to measure the data of memory behavior,than compared the different escape latency between groups before and after operation to make it clear whether the rats in model group dementia.The brain of rats were made into homogenate, than ELISA was given to measure the NF155 expression of every group.Result:There were no significant differences between the model group and the sham control group before modeling(P>0.05).After modeling, the escape latency of 30 days after surgery group were significantly longer than the sham control group in the sixth day(t=2.667,6.812,8.743,10.775;P=0.014,0.000, 0.000,0.000);60 days after surgery group were significantly longer than the sham group in the fifth day(t=3.016, 4.137,7.562,7.910,12.686;P=0.006,0.000,0.000,0.000,0.000).The NF155 concentration of model 30 days group and model 60 days group detected by ELISA was respectively(48.27±7.32)ng/mL and(37.65±6.88)ng/mL. Compared with the sham control group(85.07±6.75)ng/mL,the differences were statistically significant(P<0.05). This revealed that the NF155 content in the rats brain of model group significantly decreased compared with the sham control group.Conclusion:The change of NF155 content in brain may be involve in the occurrence of vascular dementia induced by chronic ischemia.
