中国医学创新
中國醫學創新
중국의학창신
Medical Innovation of China
2015年
30期
1-4
,共4页
张豫明%班素芬%余双霞%曾方银
張豫明%班素芬%餘雙霞%曾方銀
장예명%반소분%여쌍하%증방은
灵芝孢子油%前列腺癌%雄激素受体%转录激活
靈芝孢子油%前列腺癌%雄激素受體%轉錄激活
령지포자유%전렬선암%웅격소수체%전록격활
Ganoderma lucidum spore oil%Prostate cancer%Androgen receptor%Transcriptional activity
目的:研究灵芝孢子油对双氰睾酮诱导的前列腺癌LNCaP增殖、雄激素受体AR的激活和转录活性及相关激酶的影响.方法:采用质粒转染、Western blot、CCK-8细胞增殖活性检测及双色荧光素酶报告基因活性等研究灵芝孢子油对雄激素依赖的前列腺癌LNCaP细胞的作用.结果:CCK-8检测显示灵芝孢子油明显降低DHT刺激的LNCaP细胞的增殖活性,同时,Western blot检测表明灵芝孢子油降低了DHT诱导的前列腺癌LNCaP细胞中AR和蛋白激酶D的磷酸化活性,而对其本底蛋白的表达水平无影响.荧光素酶报告基因活性检测进一步表明灵芝孢子油明显抑制DHT诱导的LNCaP细胞中AR的转录激活活性.结论:灵芝孢子油可能通过抑制AR的激活及其转录活性而抑制雄激素依赖的前列腺癌细胞的增殖.
目的:研究靈芝孢子油對雙氰睪酮誘導的前列腺癌LNCaP增殖、雄激素受體AR的激活和轉錄活性及相關激酶的影響.方法:採用質粒轉染、Western blot、CCK-8細胞增殖活性檢測及雙色熒光素酶報告基因活性等研究靈芝孢子油對雄激素依賴的前列腺癌LNCaP細胞的作用.結果:CCK-8檢測顯示靈芝孢子油明顯降低DHT刺激的LNCaP細胞的增殖活性,同時,Western blot檢測錶明靈芝孢子油降低瞭DHT誘導的前列腺癌LNCaP細胞中AR和蛋白激酶D的燐痠化活性,而對其本底蛋白的錶達水平無影響.熒光素酶報告基因活性檢測進一步錶明靈芝孢子油明顯抑製DHT誘導的LNCaP細胞中AR的轉錄激活活性.結論:靈芝孢子油可能通過抑製AR的激活及其轉錄活性而抑製雄激素依賴的前列腺癌細胞的增殖.
목적:연구령지포자유대쌍청고동유도적전렬선암LNCaP증식、웅격소수체AR적격활화전록활성급상관격매적영향.방법:채용질립전염、Western blot、CCK-8세포증식활성검측급쌍색형광소매보고기인활성등연구령지포자유대웅격소의뢰적전렬선암LNCaP세포적작용.결과:CCK-8검측현시령지포자유명현강저DHT자격적LNCaP세포적증식활성,동시,Western blot검측표명령지포자유강저료DHT유도적전렬선암LNCaP세포중AR화단백격매D적린산화활성,이대기본저단백적표체수평무영향.형광소매보고기인활성검측진일보표명령지포자유명현억제DHT유도적LNCaP세포중AR적전록격활활성.결론:령지포자유가능통과억제AR적격활급기전록활성이억제웅격소의뢰적전렬선암세포적증식.
Objective:To investigate the role of ganoderma lucidum spore oil on tumor cell proliferation, androgen receptor activation and transcriptional activity as well as related kinase activation in prostate LNCaP cancer cells. Method:The effects of ganoderma spore oil on the LNCaP cells of the prostate cancer cells were studied by using plasmid transfection,Western blot,CCK-8 cell proliferation assay and dual luciferase reporter gene activity.Result:LNCaP cancer cells proliferation induced by DHT was significantly reduced by ganoderma lucidum spore oil. At the same time, the Western blot assay showed that ganoderma spore oil decreased the phosphorylation activity of AR and protein kinase D in prostate cancer LNCaP cells, but had no effect on the expression of the protein kinase DHT. Luciferase reporter gene activity assay indicated that the activity of AR in LNCaP cells induced by DHT was significantly inhibited by Ganoderma spore oil.Conclusion:Ganoderma lucidum spore oil may contributes to LNCaP cancer cells growth inhibition through down-regulation of AR activation and transcriptional activity in androgen dependent prostate cancer cells.
