CAJ | 학술논문

目的：探究乙型肝炎病毒（HBV）感染后并发原发性肝癌患者外周血清白介素-12（IL-12）、白介素-17（IL-17）水平检测及临床意义。方法将112例感染HBV患者分为乙型肝炎肝硬化组、原发性肝癌无远处侵犯组和原发性肝癌远处侵犯组（包括门静脉侵犯）3组，同时以健康查体人群作为对照组。采用双抗体夹心法酶联免疫吸附试验（ELISA）测定不同组人群外周血清IL-12，IL-17的含量，并进行统计学分析。结果乙型肝炎肝硬化组、原发性肝癌组与对照组比较，IL-17增高，IL-12降低，差异具有统计学意义（P＜0．05）。原发性肝癌两组之间比较，IL-17继续增高，IL-12仍降低，差异具有统计学意义（P＜0．05）。结论 IL-12，IL-17参与了HBV感染所致肝硬化及原发性肝癌的发病及病情进展的过程，为原发性肝癌患者治疗效果的评价提供实验依据。
목적：탐구을형간염병독（HBV）감염후병발원발성간암환자외주혈청백개소-12（IL-12）、백개소-17（IL-17）수평검측급림상의의。방법장112례감염HBV환자분위을형간염간경화조、원발성간암무원처침범조화원발성간암원처침범조（포괄문정맥침범）3조，동시이건강사체인군작위대조조。채용쌍항체협심법매련면역흡부시험（ELISA）측정불동조인군외주혈청IL-12，IL-17적함량，병진행통계학분석。결과을형간염간경화조、원발성간암조여대조조비교，IL-17증고，IL-12강저，차이구유통계학의의（P＜0．05）。원발성간암량조지간비교，IL-17계속증고，IL-12잉강저，차이구유통계학의의（P＜0．05）。결론 IL-12，IL-17삼여료HBV감염소치간경화급원발성간암적발병급병정진전적과정，위원발성간암환자치료효과적평개제공실험의거。
[ ABSTRACT] Objective To research the expression levels and clinical significance of serum levels of interleu-kin-12(IL-12),interleukin-17(IL-17) in primary liver cancer patients infected by HBV.Methods 112 patients infec-ted by HBV were divided into 3 groups:hepatitis B cirrhosis group,primary liver cancer without distant invasion,and pri-mary liver cancer with the distant invasion group( including portal vein invasion) ,at the same time,healthy people were taken as a control group.The contents of IL-17 and IL-12 in peripheral blood of different groups were measured by en-zyme-linked immunosorbent assay( ELISA) .Results IL-17 was higher and IL-12 was lower and the difference was sta-tistically significant(P<0.05).Compared with the 2 groups of primary liver cancer,with the progress of the tumor,IL-17 continued to increase,IL-12 was still lower,the difference was statistically significant(P<0.05).Conclusion IL-12 and IL-17 are involved in the pathogenesis and progression of liver cirrhosis and primary liver cancer caused by HBV in-fection.This study provided an experimental basis for the evaluation of the treatment effect of the patients with primary liver cancer.