CAJ | 학술논문

目的：探讨外周血中P16、E-cad和hMLH1基因启动子甲基化在胃癌早期诊断中的价值。方法收集83例早期胃癌和37例癌前病变及20例健康人群的血浆，提取血浆标本的DNA，采用甲基化特异性PCR（MSP），检测血浆标本中P16、E-cad和hMLH1基因启动子甲基化状态，分析外周血标本中P16、E-cad和hMLH1基因启动子甲基化与其临床病理的关系。结果胃癌外周血标本中 P16、E-cad 和 hMLH1基因启动子甲基化率分别为51．81％、46．99％和43．37％；胃癌前病变外周血组分别为37．84％、29．72％和29．72％；正常对照组分别为0、0和5％。 P16、E-cad和hMLH1三个基因联合检测时胃癌与癌前病变组甲基化率分别为77．11％和56．76％。 P16、E-cad和hMLH1基因启动子甲基化状态与早期胃癌患者的性别、年龄、肿瘤大小、淋巴结转移及TNM分期无关（P＞0．05）。结论 P16、E-cad和hMLH1基因启动子甲基化水平在早期胃癌外周血中升高，通过联合检测患者P16、E-cad和hMLH1基因启动子甲基化有可能成为胃癌早期诊断的指标。
목적：탐토외주혈중P16、E-cad화hMLH1기인계동자갑기화재위암조기진단중적개치。방법수집83례조기위암화37례암전병변급20례건강인군적혈장，제취혈장표본적DNA，채용갑기화특이성PCR（MSP），검측혈장표본중P16、E-cad화hMLH1기인계동자갑기화상태，분석외주혈표본중P16、E-cad화hMLH1기인계동자갑기화여기림상병리적관계。결과위암외주혈표본중 P16、E-cad 화 hMLH1기인계동자갑기화솔분별위51．81％、46．99％화43．37％；위암전병변외주혈조분별위37．84％、29．72％화29．72％；정상대조조분별위0、0화5％。 P16、E-cad화hMLH1삼개기인연합검측시위암여암전병변조갑기화솔분별위77．11％화56．76％。 P16、E-cad화hMLH1기인계동자갑기화상태여조기위암환자적성별、년령、종류대소、림파결전이급TNM분기무관（P＞0．05）。결론 P16、E-cad화hMLH1기인계동자갑기화수평재조기위암외주혈중승고，통과연합검측환자P16、E-cad화hMLH1기인계동자갑기화유가능성위위암조기진단적지표。
Objective To detect the value of P16、E-cad and hMLH1 gene promoter methylation in early detection of gastric carcinoma( GC) .Methods 83 patients with GC,37 patients with precancerous lesion and 20 normal controls were col-lected.The methylation statuses of P16、E-cad and hMLH1 in these plasma samples were determined using methylation specific PCR method.Results The methylation rates of P16、E-cad and hMLH1 in GC plasma were 51.81%、46.99%and 43.37%,re-spectively,which were significantly higher than those in plasma from patients with precancerous lesion (37.84%、29.72% and 29.72%,P<0.05) and normal controls (0、0 and 0,P<0.05).Methylation rates of the combination analysis of P16、E-cad and hMLH1 in patients with GC and precancerous lesion were 77.11%、56.76%,respectively.No significant associations existed be-tween the methylation status of the 3 genes and clinical characteristics included sex,age,tumor diameter,lymph node metastases, and TNM stage(P>0.05).Conclusion DNA methylation level of P16、E-cad and hMLH1 of plasma are significantly higher in GC patients than patients with precancerous lesion.The combination analysis of P16、E-cad and hMLH1 of the plasma DNA meth-ylation can be indicator for early diagnosis of GC.