CAJ | 학술논문

利用黄喉拟水龟(Mauremys mutica)微卫星标记,筛选出16对微卫星引物,通过优化各引物比例、荧光接头浓度、退火温度和循环次数,建立了基于2组各含8个微卫星位点多重 PCR体系的黄喉拟水龟亲子鉴定技术。应用2组微卫星多重PCR体系,通过ABI3130遗传分析仪以及cervus3.0软件对428只黄喉拟水龟进行了个体基因型检测和遗传多样性分析,结果显示,群体的平均等位基因数为14.190,平均多态信息含量为0.748,平均观测杂合度和期望杂合度分别为0.687、0.771。对89只候选母本及296只子代进行亲子鉴定分析,结果显示：在父本信息未知时,母本鉴定率为87%；母本获得的子代个数范围为1—12,个体间表现出巨大的差异,这为选择育种提供了物质基础。黄喉拟水龟多重 PCR 亲子鉴定技术的建立为群体遗传多样性分析、家系鉴定管理和选择育种提供了有效的技术手段。
이용황후의수구(Mauremys mutica)미위성표기,사선출16대미위성인물,통과우화각인물비례、형광접두농도、퇴화온도화순배차수,건립료기우2조각함8개미위성위점다중 PCR체계적황후의수구친자감정기술。응용2조미위성다중PCR체계,통과ABI3130유전분석의이급cervus3.0연건대428지황후의수구진행료개체기인형검측화유전다양성분석,결과현시,군체적평균등위기인수위14.190,평균다태신식함량위0.748,평균관측잡합도화기망잡합도분별위0.687、0.771。대89지후선모본급296지자대진행친자감정분석,결과현시：재부본신식미지시,모본감정솔위87%；모본획득적자대개수범위위1—12,개체간표현출거대적차이,저위선택육충제공료물질기출。황후의수구다중 PCR 친자감정기술적건립위군체유전다양성분석、가계감정관리화선택육충제공료유효적기술수단。
In this study, we established a technique for the parentage assignment in Asian Yellow Pond Turtles (Maure-mys mutica) based on two multiplex PCR panels. The primer ratio, the concentration of the fluorescent joint, the an-nealing temperature and the number of cycles were optimized in this technique. Each multiplex PCR panel had eight loci, 10 of which were developed in our lab and the other 6 were chosen from published literatures. With the multiplex PCR tool we performed the genotyping and the genetic diversity analysis on 428 individuals using ABl3I30 genetic analyzer and Cervus 3.0 software. The results showed that the average number of alleles was 14.190; the average polymorphism information content (PIC) was 0.748; the average observed heterozygosity (Ho) and expected heterozygosity (He) were 0.687 and 0.771 respectively. The parentage assignment was performed on 89 candidate female parents and 296 off-spring. Without information on the male parent, the identification rate was 87%. The numbers of offspring of different female parents were remarkably various, ranging from 1 to 12. This provided the material foundation for the selec-tive breeding. This technique could be a highly efficient tool for the genetic diversity analysis, the marker-assisted fa-mily identification and management, and the selective breeding of Asian Yellow Pond Turtles.