CAJ | 학술논문

为了解温度及氮磷浓度对平裂藻和栅藻生长及竞争的影响,分别在18、25和35℃条件下,以 BG11培养基为基础,设置了不同的氮磷浓度组,进行了2种藻的单独培养试验和混合培养试验,结果表明：在纯培养条件下,低营养浓度组(磷浓度分别为0.1和0.5 mg/L)平裂藻在3种温度下基本停止生长,高营养盐浓度组(磷浓度分别为1.0和1.5 mg/L)除18℃1.0 mg/L组停止生长外,其余均能正常生长增殖,且细胞最大密度表现为25℃>18℃>35℃,1.5和1.0 mg/L浓度组细胞生长差异不显著。栅藻在纯培养条件下,细胞密度总体表现为随着营养盐浓度升高而增加的趋势,细胞最大密度表现为18℃>25℃>35℃。在混合培养试验中,除35℃、1.5 mg/L组平裂藻对栅藻的竞争抑制参数大于栅藻对平裂藻的竞争抑制参数外,其余试验组均表现为栅藻受平裂藻影响较小。在混合培养体系中,0.1 mg/L组平裂藻仍基本停止生长,0.5 mg/L组受栅藻的刺激作用,生长优于纯培养体系。1.0和1.5 mg/L组均受栅藻的抑制作用,且浓度越高抑制作用越强。
위료해온도급담린농도대평렬조화책조생장급경쟁적영향,분별재18、25화35℃조건하,이 BG11배양기위기출,설치료불동적담린농도조,진행료2충조적단독배양시험화혼합배양시험,결과표명：재순배양조건하,저영양농도조(린농도분별위0.1화0.5 mg/L)평렬조재3충온도하기본정지생장,고영양염농도조(린농도분별위1.0화1.5 mg/L)제18℃1.0 mg/L조정지생장외,기여균능정상생장증식,차세포최대밀도표현위25℃>18℃>35℃,1.5화1.0 mg/L농도조세포생장차이불현저。책조재순배양조건하,세포밀도총체표현위수착영양염농도승고이증가적추세,세포최대밀도표현위18℃>25℃>35℃。재혼합배양시험중,제35℃、1.5 mg/L조평렬조대책조적경쟁억제삼수대우책조대평렬조적경쟁억제삼수외,기여시험조균표현위책조수평렬조영향교소。재혼합배양체계중,0.1 mg/L조평렬조잉기본정지생장,0.5 mg/L조수책조적자격작용,생장우우순배양체계。1.0화1.5 mg/L조균수책조적억제작용,차농도월고억제작용월강。
In order to understand the role of the temperature and the concentration of N and P in the growth ofMeris-mopedia andScenedesmus, we carried out the monoculture and co-culture ofMerismopedia andScenedesmus using BG11 medium in this study. Nutrients at different concentrations were added into the medium and the subjects were cultured at different temperatures. In the monoculture system,Merismopediastopped growing at the test temperatures when the concentration of TP was 0.1 mg/L and 0.5 mg/L, but the growth was normal when the concentration of TP was 1.0 mg/L and 1.5 mg/L. The optimal temperature for the cell growth was 25℃, followed by 35℃ and 18℃ with TP at various concentrations. The cell density ofScenedesmusbecame higher along with the increase in the concentration of nutrients in the monoculture system, and the optimal temperature for the cell grew was 18℃, followed by 25℃ and 35℃ with TP at various concentrations. In the co-culture system,Merismopedia had little effect onScenedesmusunder most conditions, except that the competition ability ofScenedesmus was stronger than that ofMerismopedia when the concentration of TP was 1.5 mg/L at 35℃. Merismopedia was seriously affected byScenedesmus,and it was suppressed under most conditions but was promoted when the concentration of TP was 0.5 mg/L.