CAJ | 학술논문

为探明沉水植物恢复对湖泊沉积物中产甲烷菌的影响，本文选用杭州西湖沉水植物恢复区域进行研究，同时对比分析未进行沉水植物恢复区域。产甲烷菌多样性和群落结构分析采用限制性片段长度多态性分析(PCR-RFLP)及分子克隆等技术。研究结果表明，沉水植物恢复区产甲烷菌OTU类型均数为13种，高于沉水植物匮乏区域的9种；并且沉水植物恢复区的Shannon指数、Simpson多样性指数较对照区分别高21.7%和9.5%，表明沉水植物的恢复有利于提高沉积物中产甲烷菌群落结构多样性。就产甲烷菌种类而言，沉水植物的恢复对甲烷微菌纲 Methanomicrobia 等影响不显著，恢复区和匮乏区分别占43.6%—67.4%、41.5%—58.1%。甲烷球菌纲Methanococci在沉水植物恢复区和匮乏区的存在量较少，分别占1.7%和4.1%。而沉水植物的恢复一定程度上提高甲烷杆菌纲Methanobacteria的比例，较匮乏区高14.6%，且仅在恢复区检测到甲烷杆状热菌科Methanothermaceae和甲烷八叠球菌科Methanosarcinaceae。
위탐명침수식물회복대호박침적물중산갑완균적영향，본문선용항주서호침수식물회복구역진행연구，동시대비분석미진행침수식물회복구역。산갑완균다양성화군락결구분석채용한제성편단장도다태성분석(PCR-RFLP)급분자극륭등기술。연구결과표명，침수식물회복구산갑완균OTU류형균수위13충，고우침수식물궤핍구역적9충；병차침수식물회복구적Shannon지수、Simpson다양성지수교대조구분별고21.7%화9.5%，표명침수식물적회복유리우제고침적물중산갑완균군락결구다양성。취산갑완균충류이언，침수식물적회복대갑완미균강 Methanomicrobia 등영향불현저，회복구화궤핍구분별점43.6%—67.4%、41.5%—58.1%。갑완구균강Methanococci재침수식물회복구화궤핍구적존재량교소，분별점1.7%화4.1%。이침수식물적회복일정정도상제고갑완간균강Methanobacteria적비례，교궤핍구고14.6%，차부재회복구검측도갑완간상열균과Methanothermaceae화갑완팔첩구균과Methanosarcinaceae。
In this study, we investigated the impacts of submerged macrophytes rehabilitation (SMR) on the methano-genic community structure in the lake sediments. We compared the diversity and structure of methanogenic populations between the SMR areas and the plant scarce areas in the West Lake of Hangzhou by using restriction fragment length polymorphism (PCR-RFLP) and molecular cloning techniques. The results showed that the average OTU type number in the SMR areas was 13, and the number was 9 in the scarce areas. Shannon-Weaver diversity and Simpson’s evenness in the SMR areas were 21.7% and 9.5% respectively, which were higher than those in the control areas. This indicated that the diversity of the methanogenic community structure could be improved by the rehabilitation of submerged macro-phytes. The in-depth analysis suggested that SMR had no significant effect on Methanomicrobia that accounted for 43.6%—67.4% and 41.5%—58.1% in the SMR areas and the scarce areas respectively. Methanococci was rare in both the SMR areas (1.7%) and the scarce areas (4.1%). We also found that the proportion of Methanobacteria in the SMR areas was 14.6% higher than in the scarce areas.Methanothermaceae andMethanosarcinaceae were only detected in the SMR areas.