CAJ | 학술논문

目的利用代谢组学技术研究不同年龄段健康人的代谢表型和生化组成差异. 方法采用气相色谱-质谱联用仪(GC/MS)对37例健康青年人和19例健康中老年人的血清和尿样中内源性小分子代谢物进行全面检测,利用多变量数据分析和方差分析方法分析两组间差异. 结果两组在偏最小二乘-判别式分析(PLS-DA)得分图中可直观清晰地分簇,且得分图参数与年龄呈线性相关.血清和尿样中与年龄相关的代谢物及变化趋势有相同之处也有各自的特性,中老年人血清中大部分代谢物较青年人血清中降低,两组间差异有统计学意义,并鉴定出结构的化合物为32个(5个,P＜0.05;27个,P＜0.01),而尿液中代谢物均升高,两组间差异有统计学意义,并鉴定出结构的化合物为29个(3个,P＜0.05;26个,P＜0.01),其中部分代谢物与临床常规实验室检查指标呈线性相关.生化代谢通路分析发现年龄相关的代谢物涉及氨基酸代谢、糖代谢、脂代谢、核苷酸代谢、三羧酸循环、肌醇代谢等,还与氧化应激相关. 结论代谢组学技术是探索年龄相关人类特征代谢谱的有前景的工具,也为进一步研究疾病发展的生物学本质和药物治疗作用奠定基础.
목적 이용대사조학기술연구불동년령단건강인적대사표형화생화조성차이. 방법 채용기상색보-질보련용의(GC/MS)대37례건강청년인화19례건강중노년인적혈청화뇨양중내원성소분자대사물진행전면검측,이용다변량수거분석화방차분석방법분석량조간차이. 결과 량조재편최소이승-판별식분석(PLS-DA)득분도중가직관청석지분족,차득분도삼수여년령정선성상관.혈청화뇨양중여년령상관적대사물급변화추세유상동지처야유각자적특성,중노년인혈청중대부분대사물교청년인혈청중강저,량조간차이유통계학의의,병감정출결구적화합물위32개(5개,P＜0.05;27개,P＜0.01),이뇨액중대사물균승고,량조간차이유통계학의의,병감정출결구적화합물위29개(3개,P＜0.05;26개,P＜0.01),기중부분대사물여림상상규실험실검사지표정선성상관.생화대사통로분석발현년령상관적대사물섭급안기산대사、당대사、지대사、핵감산대사、삼최산순배、기순대사등,환여양화응격상관. 결론 대사조학기술시탐색년령상관인류특정대사보적유전경적공구,야위진일보연구질병발전적생물학본질화약물치료작용전정기출.
Objective To investigate the metabolic phenotype or biochemical compositionvariations of healthy subjects across age groups by metabonomics.Methods Endogenous metabolites in serum and urine were comprehensively detected by gas chromatography/ mass spectrometry (GC/MS) in two groups of 37 healthy young subjects versus 19 healthy elderly subjects, and their difference between the two groups was analyzed by multivariate statistical technique and MANOVA.Results The two groups were visually clustered in partial least squares projection to latent structures and discriminant analysis(PLS-DA) scores plots, and the linear correlation between the parameters of the scores plot and age were observed.Age-related metabolites and their variation tendency in serum and urine had homogeneity and their own characteristics.Most metabolites in sera were decreased in elderly subjects as compared with young subjects (P＜0.05).However, all metabolites in urine of healthy elderly subjects were increased (P＜0.05).Additionally, parts of these metabolites were linear related with indexes of clinical routine laboratory examination.Biochemical metabolic pathway analysis indicated that age-related metabolites were involved in amino acid metabolism, saccharometabolism, lipid metabolism, nucleoside metabolism, tricarboxylic acid cycle,inositol metabolism, etcetera, and were in relationship with oxidative stress.Conclusions Metabonomics may be a promising tool to investigate the age-related characteristic metabolic profile of human,and also could lay the foundations for further study on the biological nature of disease development and on pharmacodynamic action of drugs.