CAJ | 학술논문

为了更好地了解中国鲎(Tachpleus tridentatus)和圆尾鲎(Carcinoscorpius rotundicauda)血淋巴细胞的种类组成和特征差异，综合运用光学显微镜、扫描电镜和粒度仪，较为系统地对两种鲎的血淋巴细胞进行了分类和特征研究，从而为两种鲎的血淋巴细胞和分子生物学研究提供基础资料。根据血淋巴细胞大小、核质比、细胞着色特点、细胞中颗粒存在与否、颗粒的密集程度等，中国鲎和圆尾鲎的血淋巴细胞均可分为大颗粒细胞、小颗粒细胞和透明细胞三种主要类型，且两种鲎的血淋巴细胞均以颗粒细胞为主，透明细胞在血淋巴细胞中所占比例最小，但具有高核质比。两种鲎的同类血淋巴细胞在染色和形态上无显著性差异，但在同一种鲎中，血淋巴细胞密度存在显著的雌雄差异。
위료경호지료해중국후(Tachpleus tridentatus)화원미후(Carcinoscorpius rotundicauda)혈림파세포적충류조성화특정차이，종합운용광학현미경、소묘전경화립도의，교위계통지대량충후적혈림파세포진행료분류화특정연구，종이위량충후적혈림파세포화분자생물학연구제공기출자료。근거혈림파세포대소、핵질비、세포착색특점、세포중과립존재여부、과립적밀집정도등，중국후화원미후적혈림파세포균가분위대과립세포、소과립세포화투명세포삼충주요류형，차량충후적혈림파세포균이과립세포위주，투명세포재혈림파세포중소점비례최소，단구유고핵질비。량충후적동류혈림파세포재염색화형태상무현저성차이，단재동일충후중，혈림파세포밀도존재현저적자웅차이。
In the present study, light microscopy, scanning electron microscopy and particle size analyzer were utilized to investigate hemocyte classification and characterization between two horseshoe crabs Tachpleus tridentatus and Car-cinoscorpius rotundicauda. Hemocyte classification was based on cell size, nuclear-cytoplasmic (N/C) ratio, the mor-phology, and the number and size of cytoplasmic granules in the hemocytes. Three major types of hemocytes were dis-tinguished in the two horseshoe crabs: the granular cell (GC), the semi-granular cell (SGC) and the hyaline cell (HC). GC was the major cell type in both horseshoe crabs; HC was the minor cell type with highest N/C ratio. The hemocyte types between the two horseshoe crabs had no significant differencesin staining and morphology. However, significant differences of total hemocyte counts were observed between males and females within each horseshoe crab species.