武警医学
武警醫學
무경의학
Medical Journal of the Chinese People's Armed Police Forces
2015年
11期
1087-1090
,共4页
赵彦功%舒清明%程芮%白皛%罗敏%段媛媛
趙彥功%舒清明%程芮%白皛%囉敏%段媛媛
조언공%서청명%정예%백효%라민%단원원
脑损伤%大鼠%SAX2蛋白质芯片%血清%海马
腦損傷%大鼠%SAX2蛋白質芯片%血清%海馬
뇌손상%대서%SAX2단백질심편%혈청%해마
brain injury%rat%protein chip%serum%hippo-campus
目的:研究闭合性颅脑损伤大鼠血清和海马SAX2蛋白质表达谱,探寻脑损伤生物标志蛋白。方法选72只雄性SD大鼠随机分为手术对照组及损伤后4、8、12、24、48 h组,复制Marmarou落体打击大鼠脑损伤模型,分别进行常规HE染色、尼氏染色;并用强阴离子交换芯片( SAX2)质谱技术分析大鼠闭合性脑损伤后不同时间血清和海马中蛋白质表达谱的改变。结果(1) SAX2芯片共获得530个血清蛋白质峰,147个海马蛋白质峰,6个组中,SAX2芯片在血清和海马中都能捕获的蛋白质数是14个。(2)血清中检测到的差异蛋白质,损伤后共有45个蛋白质峰的相对强度与手术对照组比较差异有统计学意义(P<0.05);另有97个蛋白质峰在对照组中未检测到。(3)海马中检测到的差异蛋白质,损伤后共有18个蛋白质峰的相对强度与手术对照组比较差异有统计学意义(P<0.05);另有40个蛋白质峰在对照组中未检测到。(4)在SAX2芯片上,血清和海马中均能检测到3656 Da这一蛋白质,该蛋白质在血清中于损伤后4 h可以检测到,在海马中于损伤后12 h可检测到。结论脑损伤可引起血清和海马SAX2蛋白质表达谱变化;血清和海马SAX2蛋白质表达谱存在差异,提示血清中出现的部分蛋白质可能为脑损伤诱导的血细胞基因表达产物;在血清和海马中检测到的差异蛋白质以及损伤后新出现的蛋白质峰,可能是脑损伤生物标志蛋白。
目的:研究閉閤性顱腦損傷大鼠血清和海馬SAX2蛋白質錶達譜,探尋腦損傷生物標誌蛋白。方法選72隻雄性SD大鼠隨機分為手術對照組及損傷後4、8、12、24、48 h組,複製Marmarou落體打擊大鼠腦損傷模型,分彆進行常規HE染色、尼氏染色;併用彊陰離子交換芯片( SAX2)質譜技術分析大鼠閉閤性腦損傷後不同時間血清和海馬中蛋白質錶達譜的改變。結果(1) SAX2芯片共穫得530箇血清蛋白質峰,147箇海馬蛋白質峰,6箇組中,SAX2芯片在血清和海馬中都能捕穫的蛋白質數是14箇。(2)血清中檢測到的差異蛋白質,損傷後共有45箇蛋白質峰的相對彊度與手術對照組比較差異有統計學意義(P<0.05);另有97箇蛋白質峰在對照組中未檢測到。(3)海馬中檢測到的差異蛋白質,損傷後共有18箇蛋白質峰的相對彊度與手術對照組比較差異有統計學意義(P<0.05);另有40箇蛋白質峰在對照組中未檢測到。(4)在SAX2芯片上,血清和海馬中均能檢測到3656 Da這一蛋白質,該蛋白質在血清中于損傷後4 h可以檢測到,在海馬中于損傷後12 h可檢測到。結論腦損傷可引起血清和海馬SAX2蛋白質錶達譜變化;血清和海馬SAX2蛋白質錶達譜存在差異,提示血清中齣現的部分蛋白質可能為腦損傷誘導的血細胞基因錶達產物;在血清和海馬中檢測到的差異蛋白質以及損傷後新齣現的蛋白質峰,可能是腦損傷生物標誌蛋白。
목적:연구폐합성로뇌손상대서혈청화해마SAX2단백질표체보,탐심뇌손상생물표지단백。방법선72지웅성SD대서수궤분위수술대조조급손상후4、8、12、24、48 h조,복제Marmarou락체타격대서뇌손상모형,분별진행상규HE염색、니씨염색;병용강음리자교환심편( SAX2)질보기술분석대서폐합성뇌손상후불동시간혈청화해마중단백질표체보적개변。결과(1) SAX2심편공획득530개혈청단백질봉,147개해마단백질봉,6개조중,SAX2심편재혈청화해마중도능포획적단백질수시14개。(2)혈청중검측도적차이단백질,손상후공유45개단백질봉적상대강도여수술대조조비교차이유통계학의의(P<0.05);령유97개단백질봉재대조조중미검측도。(3)해마중검측도적차이단백질,손상후공유18개단백질봉적상대강도여수술대조조비교차이유통계학의의(P<0.05);령유40개단백질봉재대조조중미검측도。(4)재SAX2심편상,혈청화해마중균능검측도3656 Da저일단백질,해단백질재혈청중우손상후4 h가이검측도,재해마중우손상후12 h가검측도。결론뇌손상가인기혈청화해마SAX2단백질표체보변화;혈청화해마SAX2단백질표체보존재차이,제시혈청중출현적부분단백질가능위뇌손상유도적혈세포기인표체산물;재혈청화해마중검측도적차이단백질이급손상후신출현적단백질봉,가능시뇌손상생물표지단백。
Objective To study the alteration of SAX2 protein expression pattern in rat serum and hippocampus after closed head injury and to find biomarkers of brain injury.Methods Male Sprague-Dawley rats were randomly divided into sham operation group and injury groups which were subjected to Marmarou’ s brain injury and then subdivided into 4 h、8 h、12 h、24 h and 48 h groups according to the time elapsed after injury.The pathological study included H&E staining and toluidine blue staining.The change of protein expression pattern in serum and hippocampus were monitored with strong anion exchange chips (SAX2).Results (1)A total of 530 protein peaks were detected on SAX2 chips in serum,and a total of 147 protein peaks in hippocampus.(2) Among the total of protein peaks detected, the relative intensity of 45 protein peaks was shown to be differentially altered on SAX2 chips in serum of injury groups, compared with sham operation group(P<0.05).There were 97 protein peaks in serum of injury groups, which could not be detected in sham operation group with SAX2 chips.(3) The relative intensity of 18 protein peaks was shown to be differentially altered on SAX2 chips in hippocampus of injury groups compared with sham operation group(P<0.05).There were 40 protein peaks in hip-pocampus of injury groups which could not be detected in sham operation group with SAX2 chips.(4) On SAX2 chips, only one pro-tein(3656Da) peak was detected not only in serum but also in hippocampus.Conclusions The alteration of SAX2 protein expression patterns in serum and hippocampus can be induced after brain injury.The SAX2 protein expression patterns of serum and hippocampus after brain injury are different, which suggests that part of protein in serum can be a production of blood cells gene expression induced by brain injury.The distinct proteins detected in serum and hippocampus includ the proteins which are significant, compared with sham operation group and those which are not detected in sham operation group.They can be biomarkers of brain injury.