CAJ | 학술논문

目的：分析葡萄籽乙醇提取液对医院临床分离菌株的体外抑菌作用和毒性。方法以临床标本分离菌株为对象，采用抑菌环试验和营养肉汤稀释法对其抑菌效果进行了试验观察；采用动物试验对其进行了毒性试验观察。结果含葡萄籽生药125 g/ L 的乙醇提取液对临床分离菌株和标准菌株的抑菌环直径均大于7 mm。葡萄籽乙醇提取物对白色念球菌的最低抑菌浓度（MIC）为25.0 g/ L。小鼠急性经口 LD50值大于5000 mg/ kg，葡萄籽提取原液对家兔皮肤刺激试验指数为0.13，属于轻度刺激性。结论葡萄籽乙醇提取液具有良好的抑菌作用，属无毒、低刺激性物质。
목적：분석포도자을순제취액대의원림상분리균주적체외억균작용화독성。방법이림상표본분리균주위대상，채용억균배시험화영양육탕희석법대기억균효과진행료시험관찰；채용동물시험대기진행료독성시험관찰。결과함포도자생약125 g/ L 적을순제취액대림상분리균주화표준균주적억균배직경균대우7 mm。포도자을순제취물대백색념구균적최저억균농도（MIC）위25.0 g/ L。소서급성경구 LD50치대우5000 mg/ kg，포도자제취원액대가토피부자격시험지수위0.13，속우경도자격성。결론포도자을순제취액구유량호적억균작용，속무독、저자격성물질。
Objective:To analyze the bacteriostatic efficacy and toxicity of grape seeds ethanol extract in hospital clini-cally isolated strain. Methods:Clinically isolated strain specimens were used to observe the bacteriostatic efficacy of grape seed ethanol extract via bacterial inhibition zone test and nutrient broth method;and animal test was employed to observe experimentally its toxicity. Results:The diameters of bacterial inhibition zones of clinically isolated microorganism strain and standard microorganism strain exposed to the ethanol containing grape seed crude drug 125 mg/ L were larger than 7 mm. The minimum inhibition concentration( MIC)of grape seed ethanol extract for C albicans was 25. 0 mg/ ml. The mouse acute oral LD50 was above 5000 mg/ kg,the rabbit skin irritation test index of grape seed stock solution was 0. 13, which belonged to mild irritation. Conclusion:Grape seed extract has good bacteriostatic efficacy and it belongs to practi-cally non-toxic class and was slightly irritative to skin.