CAJ | 학술논문

为了解乌鳢（ Channa argus）性别分化相关机制，采用RT-PCR和RACE法克隆了乌鳢脑型芳香化酶基因Cyp19a1b cDNA部分序列，运用半定量RT-PCR和qPCR技术检测其在雌雄个体组织中的表达情况。结果显示，乌鳢Cyp19a1b cDNA部分序列为1432 bp，包括120 bp 3′-UTR和1311 bp编码区，编码436个氨基酸。序列比对表明乌鳢Cyp19a1b氨基酸序列包含三个功能保守区，与毛足鲈、平鲷和金钱鱼Cyp19a1b同源性较高，分别为89％、88％和88％。半定量RT-PCR和qPCR结果显示，乌鳢Cyp19a1b基因在雌、雄成鱼垂体中表达量高，在肠组织中表达量低，在雄鱼脑组织中呈现高表达而在雌鱼脑组织中则未表达，在其他组织均未检测到表达，表明乌鳢Cyp19a1b基因的表达存在组织特异性及功能差异。
위료해오례（ Channa argus）성별분화상관궤제，채용RT-PCR화RACE법극륭료오례뇌형방향화매기인Cyp19a1b cDNA부분서렬，운용반정량RT-PCR화qPCR기술검측기재자웅개체조직중적표체정황。결과현시，오례Cyp19a1b cDNA부분서렬위1432 bp，포괄120 bp 3′-UTR화1311 bp편마구，편마436개안기산。서렬비대표명오례Cyp19a1b안기산서렬포함삼개공능보수구，여모족로、평조화금전어Cyp19a1b동원성교고，분별위89％、88％화88％。반정량RT-PCR화qPCR결과현시，오례Cyp19a1b기인재자、웅성어수체중표체량고，재장조직중표체량저，재웅어뇌조직중정현고표체이재자어뇌조직중칙미표체，재기타조직균미검측도표체，표명오례Cyp19a1b기인적표체존재조직특이성급공능차이。
In order to explore sex differentiation mechanism of Channa argus, we cloned partial cDNA sequence of Cyp19a1b gene using RT-PCR and RACE methods, and detected its expression patterns in various tissues of adult female and male individuals based on semi-quantitative RT-PCR and qPCR techniques .The results showed that the length of the partial Cyp19a1b cDNA was 1 432 bp, including a 120 bp 3′-UTR and a 1 311 bp coding region that encoded 436 amino acids.Alignment of sequences revealed that Cyp19a1b amino acid sequence of C.argus contained three functionally con-servative regions , and had a high level of homology with Trichogaster trichopterus ( 89%) , Rhabdosargus sarba ( 88%) and Scatophagus argus (88%).Semi-quantitative RT-PCR and qPCR revealed that Cyp19a1b mRNA had high levels of expression in the pituitary and low levels of expression in the intestine of both female and male .There was a high expres-sion level in the brain of male , but no expression in the brain of female and in other detected tissues of both sexes .The tissue distribution of gene expression reveals potential tissue specificity and functional differences for Cyp19a1b in C.argus.