中华实验和临床感染病杂志(电子版)
中華實驗和臨床感染病雜誌(電子版)
중화실험화림상감염병잡지(전자판)
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version)
2015年
5期
661-664
,共4页
焦宏%张辉%佟月娟%王咏红%王国亮
焦宏%張輝%佟月娟%王詠紅%王國亮
초굉%장휘%동월연%왕영홍%왕국량
过敏性紫癜肾炎%过敏性紫癜%淋巴细胞
過敏性紫癜腎炎%過敏性紫癜%淋巴細胞
과민성자전신염%과민성자전%림파세포
Henoch-sch?nlein purpura (HSP)%Nephritis%Lymphocytes
目的 基于过敏性紫癜(HSP)与上呼吸道感染的密切联系以及感染对免疫系统尤其是淋巴细胞组成的影响,探讨过敏性紫癜肾炎患儿外周血淋巴细胞亚群的变化特点及临床意义.方法 采用流式细胞术检测563例过敏性紫癜患儿外周血T淋巴细胞(CD3+、CD4+、CD8+、CD4/CD8)、B细胞(CD19+)以及NK细胞(CD16+CD56+)的水平,比较过敏性紫癜肾炎与无肾炎表现的过敏性紫癜患儿外周血淋巴细胞亚群的差异.结果 与无肾炎HSP患儿比较,肾炎HSP患儿的发病年龄较高,性别差异无统计学意义;CD3+(t =-7.077,P< 0.001)、CD4+(t =-3.558,P< 0.001)和CD8+(t =-5.326,P< 0.001)水平显著升高,但CD4/CD8无显著差异(P> 0.05),B细胞水平显著下降(t= 7.181,P< 0.001),NK细胞水平无显著差异(P> 0.05).结论 过敏性紫癜患儿存在外周血T淋巴细胞免疫功能紊乱,T淋巴细胞亚群含量的变化尤其是CD8+淋巴细胞的升高,有助于肾炎和非肾炎HSP的鉴别.深入研究感染对HSP患儿淋巴细胞组成的影响,对HSP的防治可能具有重要的意义.
目的 基于過敏性紫癜(HSP)與上呼吸道感染的密切聯繫以及感染對免疫繫統尤其是淋巴細胞組成的影響,探討過敏性紫癜腎炎患兒外週血淋巴細胞亞群的變化特點及臨床意義.方法 採用流式細胞術檢測563例過敏性紫癜患兒外週血T淋巴細胞(CD3+、CD4+、CD8+、CD4/CD8)、B細胞(CD19+)以及NK細胞(CD16+CD56+)的水平,比較過敏性紫癜腎炎與無腎炎錶現的過敏性紫癜患兒外週血淋巴細胞亞群的差異.結果 與無腎炎HSP患兒比較,腎炎HSP患兒的髮病年齡較高,性彆差異無統計學意義;CD3+(t =-7.077,P< 0.001)、CD4+(t =-3.558,P< 0.001)和CD8+(t =-5.326,P< 0.001)水平顯著升高,但CD4/CD8無顯著差異(P> 0.05),B細胞水平顯著下降(t= 7.181,P< 0.001),NK細胞水平無顯著差異(P> 0.05).結論 過敏性紫癜患兒存在外週血T淋巴細胞免疫功能紊亂,T淋巴細胞亞群含量的變化尤其是CD8+淋巴細胞的升高,有助于腎炎和非腎炎HSP的鑒彆.深入研究感染對HSP患兒淋巴細胞組成的影響,對HSP的防治可能具有重要的意義.
목적 기우과민성자전(HSP)여상호흡도감염적밀절련계이급감염대면역계통우기시림파세포조성적영향,탐토과민성자전신염환인외주혈림파세포아군적변화특점급림상의의.방법 채용류식세포술검측563례과민성자전환인외주혈T림파세포(CD3+、CD4+、CD8+、CD4/CD8)、B세포(CD19+)이급NK세포(CD16+CD56+)적수평,비교과민성자전신염여무신염표현적과민성자전환인외주혈림파세포아군적차이.결과 여무신염HSP환인비교,신염HSP환인적발병년령교고,성별차이무통계학의의;CD3+(t =-7.077,P< 0.001)、CD4+(t =-3.558,P< 0.001)화CD8+(t =-5.326,P< 0.001)수평현저승고,단CD4/CD8무현저차이(P> 0.05),B세포수평현저하강(t= 7.181,P< 0.001),NK세포수평무현저차이(P> 0.05).결론 과민성자전환인존재외주혈T림파세포면역공능문란,T림파세포아군함량적변화우기시CD8+림파세포적승고,유조우신염화비신염HSP적감별.심입연구감염대HSP환인림파세포조성적영향,대HSP적방치가능구유중요적의의.
Objective To investigate the characteristics of peripheral blood lymphocytes in pediatric henoch-sch?nlein purpura (HSP) with or without nephritis based on the close relationship between allergic purpura nephritis and upper respiratory tract infection and the impact of infection on the immune system, especially on the lymphocytes, and to provide reference for diagnosis and treatment of HSP children. Methods The amount of peripheral blood lymphocyte subsets in 563 children with HSP were analyzed, retrospectively. The levels of T cell (CD3+, CD4+, CD8+), B cell (CD19+) and NK cell (CD16+CD56+) in peripheral blood were detected by the flow cytometry. The differences between HSP with and without nephritis were analyzed, comparatively.Results Compared with HSP children without nephritis, HSP nephritis children had older age, signiifcantly increased CD3+ (t =-7.077,P < 0.001), CD4+(t =-3.558,P < 0.001), CD8+ (t =-5.326, P < 0.001) T cells and signiifcantly decreased B cells (t= 7.181,P < 0.001). There were no signiifcant difference in CD4/CD8 and NK levels between these two groups.Conclusions Peripheral T cell dysfunction exists in HSP children. The amount of T cell subtypes help to judgment whether or not there is nephritis in HSP children. Further study the impact of infection on peripheral T cell dysfunction in children with HSP will help to prevention and treatment of HSP.