中国肝脏病杂志（电子版）
中國肝髒病雜誌（電子版）
중국간장병잡지（전자판）
Chinese Journal of Liver Diseases (Electronic Version)
2015年
3期
77-80
,共4页
张旸%辛永宁%程钰婷%姜曼%陈立震%宣世英
張旸%辛永寧%程鈺婷%薑曼%陳立震%宣世英
장양%신영저%정옥정%강만%진립진%선세영
微粒体甘油三酸酯转移蛋白%单核苷酸多态性%非酒精性脂肪肝
微粒體甘油三痠酯轉移蛋白%單覈苷痠多態性%非酒精性脂肪肝
미립체감유삼산지전이단백%단핵감산다태성%비주정성지방간
Microsomal triglyceride transfer protein%Single nucleotide polymorphism%Non-alcoholic fatty liver
目的：研究微粒体甘油三酸酯转移蛋白（MTTP）基因rs1800591位点多态性与非酒精性脂肪性肝病（NAFLD）的关系。方法采用聚合酶链式反应（PCR）-限制性片段长度多态性检测219例NAFLD患者和210例正常对照组MTTP基因rs1800591位点基因型和等位基因。应用方差分析、卡方检验、非条件Logistic回归分析等方法判断NAFLD患者与正常对照组的各项指标。结果 NAFLD组与对照组两者rs1800591位点的基因型分布和等位基因频率差异有统计学意义（χ2=9.007，P =0.011；χ2=9.599，P =0.002）。经混杂因素校正后，非条件Logistic 回归分析结果显示，与TT基因型相比，GG+ GT基因型发生NAFLD的风险比值比：OR =1.623，95％CI 为1.070～1.648，P =0.030。等位基因G携带者患NAFLD的风险是T等位基因的1.521倍（OR =1.521，95% CI 为1.089～2.123，P =0.014）。TT基因型与GG + GT组ALT、LDL、TG的差异有统计学意义（ALT：P =0.001；LDL：P =0.008；TG：P =0.024）。结论青岛地区汉族人群中MTTP基因rs1800591位点多态性与患NAFLD的风险有相关性。
目的：研究微粒體甘油三痠酯轉移蛋白（MTTP）基因rs1800591位點多態性與非酒精性脂肪性肝病（NAFLD）的關繫。方法採用聚閤酶鏈式反應（PCR）-限製性片段長度多態性檢測219例NAFLD患者和210例正常對照組MTTP基因rs1800591位點基因型和等位基因。應用方差分析、卡方檢驗、非條件Logistic迴歸分析等方法判斷NAFLD患者與正常對照組的各項指標。結果 NAFLD組與對照組兩者rs1800591位點的基因型分佈和等位基因頻率差異有統計學意義（χ2=9.007，P =0.011；χ2=9.599，P =0.002）。經混雜因素校正後，非條件Logistic 迴歸分析結果顯示，與TT基因型相比，GG+ GT基因型髮生NAFLD的風險比值比：OR =1.623，95％CI 為1.070～1.648，P =0.030。等位基因G攜帶者患NAFLD的風險是T等位基因的1.521倍（OR =1.521，95% CI 為1.089～2.123，P =0.014）。TT基因型與GG + GT組ALT、LDL、TG的差異有統計學意義（ALT：P =0.001；LDL：P =0.008；TG：P =0.024）。結論青島地區漢族人群中MTTP基因rs1800591位點多態性與患NAFLD的風險有相關性。
목적：연구미립체감유삼산지전이단백（MTTP）기인rs1800591위점다태성여비주정성지방성간병（NAFLD）적관계。방법채용취합매련식반응（PCR）-한제성편단장도다태성검측219례NAFLD환자화210례정상대조조MTTP기인rs1800591위점기인형화등위기인。응용방차분석、잡방검험、비조건Logistic회귀분석등방법판단NAFLD환자여정상대조조적각항지표。결과 NAFLD조여대조조량자rs1800591위점적기인형분포화등위기인빈솔차이유통계학의의（χ2=9.007，P =0.011；χ2=9.599，P =0.002）。경혼잡인소교정후，비조건Logistic 회귀분석결과현시，여TT기인형상비，GG+ GT기인형발생NAFLD적풍험비치비：OR =1.623，95％CI 위1.070～1.648，P =0.030。등위기인G휴대자환NAFLD적풍험시T등위기인적1.521배（OR =1.521，95% CI 위1.089～2.123，P =0.014）。TT기인형여GG + GT조ALT、LDL、TG적차이유통계학의의（ALT：P =0.001；LDL：P =0.008；TG：P =0.024）。결론청도지구한족인군중MTTP기인rs1800591위점다태성여환NAFLD적풍험유상관성。
Objective To study the association between the microsomal triglyceride transfer protein (MTTP) gene and hereditary risk of non-alcoholic fatty liver disease (NAFLD). Methods Peripheral blood DNA from 219 patients diagnosed with NAFLD and 210 control subjects were used to determine the MTTP genotype by polymerase chain reaction (PCR) and direct sequencing. Inter-group differences and associations were assessed statistically using t tests, Chi square and logistic analysis. Results There were significant differences in the frequencies of rs1800591 genotype and allele between the NAFLD and control subjects (χ2 = 9.007, P = 0.011; χ2 = 9.599, P = 0.002). Logistic regression analysis adjusted for confounding factors showed that the OR of carriers with the GG + GT genotype of rs1800591 developing NAFLD was 1.623, (95%CI:1.070~1.648, P = 0.030), compared with those with TT genotype. Also, in contrast with T allele, there was an increased risk of NAFLD for rs1800591 G allele (OR = 1.521, 95%CI: 1.089~2.123, P = 0.014). The levels of ALT, LDL and TG were higher in GG + GT genotype than that in TT genotype, with significant differences. Conclusions The polymorphism rs1800591 in MTTP is related to the risk of NAFLD.
