CAJ | 학술논문

目的观察血液透析患者左心室肥厚与单核细胞趋化蛋白-1(MCP-1)基因多态性的关系,探讨血液透析患者左心室肥厚的遗传易感因素.方法选取血液透析患者142例,根据左心室重量指数结果分为肥厚组和无肥厚组,运用聚合酶链式反应限制性片段长度多态性技术(PC-RRFLP)检测血液透析患者MCP-1基因调节区-2518A/G基因多态性,分析左心室肥厚程度与MCP-1基因多态性的关系.结果 MCP-1-2518位点基因多态性在左心室肥厚组的分布:8例(8.3％)为AA基因型,50例(51.5％)为AG基因型,39例(40.2％)为GG基因型;无左心室肥厚组的分布为AA基因型7例(15.6％),AG基因型28例(62.2％),GG基因型10例(22.2％);两组基因型间x2值为5.073,P值为0.079,无统计学差异;-2518A/G等位基因频率在左心室肥厚组的分布:A等位基因占34.0％,G等位基因占66.0％;在无肥厚组的分布为:A等位基因比例为46.7％,G等位基因比例为53.3％;两组间等位基因频率比较有统计学差异(x2=4.172,P=0.041).肥厚组中MCP-1基因-2518位点G等位基因频率显著高于无肥厚组,差异具有统计学意义(P＜0.05).结论 MCP-1-2518位点等位基因G患者更易发生左心室肥厚.
목적 관찰혈액투석환자좌심실비후여단핵세포추화단백-1(MCP-1)기인다태성적관계,탐토혈액투석환자좌심실비후적유전역감인소.방법 선취혈액투석환자142례,근거좌심실중량지수결과분위비후조화무비후조,운용취합매련식반응한제성편단장도다태성기술(PC-RRFLP)검측혈액투석환자MCP-1기인조절구-2518A/G기인다태성,분석좌심실비후정도여MCP-1기인다태성적관계.결과 MCP-1-2518위점기인다태성재좌심실비후조적분포:8례(8.3％)위AA기인형,50례(51.5％)위AG기인형,39례(40.2％)위GG기인형;무좌심실비후조적분포위AA기인형7례(15.6％),AG기인형28례(62.2％),GG기인형10례(22.2％);량조기인형간x2치위5.073,P치위0.079,무통계학차이;-2518A/G등위기인빈솔재좌심실비후조적분포:A등위기인점34.0％,G등위기인점66.0％;재무비후조적분포위:A등위기인비례위46.7％,G등위기인비례위53.3％;량조간등위기인빈솔비교유통계학차이(x2=4.172,P=0.041).비후조중MCP-1기인-2518위점G등위기인빈솔현저고우무비후조,차이구유통계학의의(P＜0.05).결론 MCP-1-2518위점등위기인G환자경역발생좌심실비후.
Objectives To observe the relationship between left vcntricular hypertrophy (LVH) and monocyte chemoattractant protein-1 (MCP-1) gene polymorphism and explore the genetic susceptibility factors of LVH in hemodialysis patients.Methods 142 patient cases of hemodialysis patients were included.Dividing the cases into LVH group and non-LVH group according to the LVMI.valuing the blood stasis of 142 cases,Measuring the MCP-1 gene regulatory region-2518 A/G gene polymorphisms differences in hemodialysis patients by using the polymerase chain reaction restriction fragment length polymorphism (PC-RRFLP),the relationship between the degree of LVH and MCP-1gene polymorphism was analyzed.Results The distribution of the MCP-1-2518 gene polymorphism is 8 cases(8.3％) for the AA genotype,50 cases(51.5％) for the AG genotype,and 39 cases(40.2％) for the GG genotype in LVH group;the distribution of non-LVH group is the AA genotype 7 cases(15.6％),AG genotype 28 cases(62.2％),the GG genotypein10 cases(22.2％);no significant difference of genotypes in two groups;Allelefrequency for A and G were 34.0％ and 66.0％ in the LVH group;Allelefrequency for A and G were 46.7％ and53.3％ in the non-LVH group;allele frequencies between the two groups were significantly different(P ＜ 0.05).There was a statistically significant differencc in the relationship between MCP-1 gene polymorphism and LVH (P ＜ 0.05),LVH group of MCP-1 gene-2518G allelefrequency was significantly higher than non-LVH group.Conclusions The MCP-1-2518 G allele was more likely to have left ventricular hypertrophy.